Engineering of Glycosyltransferase for Efficient Biosynthesis of Salidroside DOI
Fucheng Zhu,

Zixu Yan,

Kexue Zhao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Salidroside has been widely used in the cosmetic and medicinal industries. Previously, potential glycosyltransferase UGTBS was obtained for salidroside synthesis. However, catalytic efficiency undesirable. In this study, a semirational design applied to engineer UGTBS. The quadruple mutant M4 (I62N/S129T/F168W/Y316S) showed significantly enhanced A conversion rate of 94.7% using M4, which 2.2-fold higher than that wild-type. regioselectivity toward tyrosol hydroxyl also improved, resulting 97.9% total product, 1.2-fold wild type. Kinetic constants molecular simulations indicated increased affinity altered conformation binding pocket accounted Furthermore, fed-batch cascade reaction strategy used, over 183 mM obtained. engineered demonstrated precise catalysis formation.

Language: Английский

Engineering of Glycosyltransferase for Efficient Biosynthesis of Salidroside DOI
Fucheng Zhu,

Zixu Yan,

Kexue Zhao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Salidroside has been widely used in the cosmetic and medicinal industries. Previously, potential glycosyltransferase UGTBS was obtained for salidroside synthesis. However, catalytic efficiency undesirable. In this study, a semirational design applied to engineer UGTBS. The quadruple mutant M4 (I62N/S129T/F168W/Y316S) showed significantly enhanced A conversion rate of 94.7% using M4, which 2.2-fold higher than that wild-type. regioselectivity toward tyrosol hydroxyl also improved, resulting 97.9% total product, 1.2-fold wild type. Kinetic constants molecular simulations indicated increased affinity altered conformation binding pocket accounted Furthermore, fed-batch cascade reaction strategy used, over 183 mM obtained. engineered demonstrated precise catalysis formation.

Language: Английский

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