Antifungal effect of o‐vanillin on mitochondria of Aspergillus flavus: ultrastructure and TCA cycle are destroyed

International Journal of Food Science & Technology, Год журнала: 2022, Номер 57(5), С. 3142 - 3149

Опубликована: Март 2, 2022

Abstract Aspergillus flavus ( A. ) is a conditioned pathogen that severely endangers food safety. This study aimed to determine the mode of action o ‐vanillin against mitochondria. First, hindered respiration. Furthermore, mitochondrial ultrastructures were damaged with scanning electron microscopy (SEM) and transmission (TEM) characterisation. The activities tricarboxylic acid (TCA) cycle‐related enzymes regulated significantly P < 0.05), most significant reduction promotion was citrate synthase (CS) malic dehydrogenase (MDH), respectively, at 100 μg mL −1 ‐vanillin. Consistent above phenotypic results, real‐time quantitative reverse transcription polymerase chain reaction (PCR) (QRT‐PCR) results showed relative expressions genes encoding TCA‐related remarkably especially for CIT1 gene (downregulated) MDH (upregulated). demonstrated damage on mitochondria shed light antifungal mechanism .

Язык: Английский

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Anti-aflatoxigenic nano-emulsions based on Monarda didyma and Neopallasia pectinata essential oils as novel green agent for food preservation

Industrial Crops and Products, Год журнала: 2022, Номер 180, С. 114777 - 114777

Опубликована: Март 10, 2022

Язык: Английский

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Thymol Induces Cell Death of Fusarium oxysporum f. sp. niveum via Triggering Superoxide Radical Accumulation and Oxidative Injury In Vitro

Agronomy, Год журнала: 2023, Номер 13(1), С. 189 - 189

Опубликована: Янв. 6, 2023

Fusarium oxysporum f. sp. niveum (FON) causes watermelon wilt that is one of the major disease-causing yield losses watermelon. Sustainable development agriculture requires controlling disease with good environmental performance. One important approach to identify environmental-friendly compounds inhibitory activity against FON. Thymol a plant-derived compound safe for ecology. Little known about application thymol in agriculture. In this study, we studied FON by using morphological, physiological, and histochemical approaches. significantly inhibited colony diameter dose-dependent manner, EC50 at 21 µg/mL. 10, 21, 35 µg/mL decreased fresh weight mycelia 29.0%, 50.6%, 69.5%, respectively. Microscopic observation revealed irregular damage loss shape upon exposure. induced accumulation superoxide radical mycelial cells accompanied increased antioxidative enzymes (SOD, dismutase; CAT, catalase). membrane permeability was indicated lipid peroxidation electrolyte leakage (increased 29–58%) cells. These results suggested oxidative mycelia, which may be possible reasons thymol-induced cell death observed fluorescent detection. production conidia germination conidia. accumulation, peroxidation, as well. All these FON, have resulted from radical-induced injury both

Язык: Английский

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Estragole Inhibits Growth and Aflatoxin Biosynthesis of Aspergillus flavus by Affecting Reactive Oxygen Species Homeostasis

Microbiology Spectrum, Год журнала: 2023, Номер 11(4)

Опубликована: Июнь 8, 2023

A variety of essential oils and edible compounds have been widely recognized for their antifungal activity in recent years. In this study, we explored the estragole from Pimenta racemosa against Aspergillus flavus investigated underlying mechanism action. The results showed that had significant A. flavus, with a minimum inhibitory concentration 0.5 μL/mL spore germination. Additionally, inhibited biosynthesis aflatoxin dose-dependent manner, was significantly at 0.125 μL/mL. Pathogenicity assays potential peanut corn grains by inhibiting conidia production. Transcriptomic analysis differentially expressed genes (DEGs) were mainly related to oxidative stress, energy metabolism, secondary metabolite synthesis following treatment. Importantly, experimentally verified reactive species accumulation downregulation antioxidant enzymes, including catalase, superoxide dismutase, peroxidase. These suggest inhibits growth modulating intracellular redox homeostasis. findings expand our knowledge on molecular mechanisms estragole, provide basis as agent contamination.

Язык: Английский

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The CfKOB1 gene related to cell apoptosis is required for pathogenicity and involved in mycovirus-induced hypovirulence in Colletotrichum fructicola

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 271, С. 132437 - 132437

Опубликована: Май 16, 2024

Язык: Английский

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