Characterization of novel cold-active chitin deacetylase for green production of bioactive chitosan
AMB Express,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 4, 2025
Abstract
A
Novel
cold-active
chitin
deacetylase
from
Shewanella
psychrophila
WP2
(SpsCDA)
was
overexpressed
in
Escherichia
coli
BL21
and
employed
for
deacetylation
of
to
chitosan.
The
produced
chitosan
characterized,
its
antifungal
activity
investigated
against
Fusarium
oxysporum
.
purified
recombinant
SpsCDA
appeared
as
a
single
band
on
SDS-PAGE
at
approximately
60
kDa,
specific
92
U/mg.
optimum
temperature
pH
were
15
°C
8.0,
respectively,
the
enzyme
significantly
enhanced
presence
NaCl.
bioconversion
by
accomplished
72
h,
yield
69.2%.
solubility
estimated
be
73.4%,
degree
78.1%.
molecular
weight
224.7
±
8.4
kDa
with
crystallinity
index
(CrI)
value
18.75.
Moreover,
FTIR
XRD
spectra
revealed
characteristic
peaks
enzymatically
compared
standard
chitosan,
indicating
their
structural
similarity.
inhibited
spore
germination
F.
minimum
inhibitory
concentration
(MIC)
1.56
mg/mL.
potential
effect
is
attributed
inhibition
accompanied
ultrastructural
damage
membranes
leakage
cellular
components,
evidenced
transmission
electron
microscopy
(TEM),
accumulation
reactive
oxygen
species
(ROS)
that
confirmed
fluorescence
microscopy.
This
study
shed
light
S.
provides
candidate
green
preparation
Язык: Английский
Inhibition Mechanism of Cinnamomum burmannii Leaf Essential Oil Against Aspergillus flavus and Aflatoxins
Huanyan Liang,
Feifei Lv,
Xian Mu
и другие.
Foods,
Год журнала:
2025,
Номер
14(4), С. 682 - 682
Опубликована: Фев. 17, 2025
This
investigation
evaluates
the
comparative
efficacy
of
Cinnamomum
burmannii
leaf
essential
oil
(YXYO)
and
its
main
active
ingredients
as
a
novel
preservative
to
protect
stored
food
commodities
from
fungal
infestations,
aflatoxin
B1
(AFB1)
contamination
caused
by
Aspergillus
flavus.
Morphological
observations
utilizing
SEM
TEM
revealed
significant
alterations
in
treated
samples,
alongside
decrease
ergosterol
content
dose-dependent
disruption
antioxidant
system
energy
system.
Transcriptomic
analysis
suggested
that
differentially
expressed
genes
were
predominantly
associated
with
spore
growth,
cell
wall,
membrane,
oxidative
stress,
metabolism,
biosynthesis.
Solid-phase
microextraction-gas
chromatography-mass
spectrometry
(SPME-GC-MS)
identified
ten
YXYO,
including
borneol,
α-terpineol,
terpinen-4-ol,
etc.
Moreover,
an
effective
inhibition
A.
flavus
infection
peanuts
was
observed
application
30
μL/disc
YXYO
blend
compounds.
Язык: Английский
Bacillus velezensis: a versatile ally in the battle against phytopathogens—insights and prospects
Applied Microbiology and Biotechnology,
Год журнала:
2024,
Номер
108(1)
Опубликована: Авг. 15, 2024
The
escalating
interest
in
Bacillus
velezensis
as
a
biocontrol
agent
arises
from
its
demonstrated
efficacy
inhibiting
both
phytopathogenic
fungi
and
bacteria,
positioning
it
promising
candidate
for
biotechnological
applications.
This
mini
review
aims
to
offer
comprehensive
exploration
of
the
multifaceted
properties
B.
velezensis,
with
particular
focus
on
beneficial
interactions
plants
potential
controlling
fungi.
molecular
dialogues
involving
plants,
phytopathogens
are
scrutinized
underscore
intricate
mechanisms
orchestrating
these
interactions.
Additionally,
elucidates
mode
action
particularly
through
cyclic
lipopeptides,
highlighting
their
importance
promoting
plant
growth.
agricultural
applications
detailed,
showcasing
role
enhancing
crop
health
productivity
while
reducing
reliance
chemical
pesticides.
Furthermore,
extends
purview
industrial
environmental
arenas,
versatility
across
various
sectors.
By
addressing
challenges
such
formulation
optimization
regulatory
frameworks,
chart
course
effective
utilization
velezensis.
•
fights
phytopathogens,
boosting
biotech
shapes
agri-biotech
future,
offers
sustainable
solutions
Explores
plant-B.
dialogue,
lipopeptide
showcased
Язык: Английский
In vitro control of Fusarium oxysporum f. sp. lycopersici Race 3 by the innovative formulation consisting of a thymol-based emulsion, and evaluation of its effects on the vigor of tomato seeds
Scientia Horticulturae,
Год журнала:
2024,
Номер
336, С. 113380 - 113380
Опубликована: Июнь 12, 2024
Язык: Английский
Magnolol inhibits Colletotrichum. gloeosporioides via disruption of plasma membrane and induction of reactive oxygen species accumulation
Food Bioscience,
Год журнала:
2024,
Номер
61, С. 104620 - 104620
Опубликована: Июнь 22, 2024
Язык: Английский
Dismantling of Necrotroph Alternaria alternata by Cellular Intervention of Peppermint oil Nanoemulsion (PNE)
Microbial Pathogenesis,
Год журнала:
2024,
Номер
unknown, С. 107041 - 107041
Опубликована: Окт. 1, 2024
Язык: Английский
The Impact of Biocontrol Bacteria on Cotton Resistance and Their Effects on Signaling Pathways Related to Defense Against Verticillium Wilt Infection
Опубликована: Янв. 1, 2024
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DOI
Язык: Английский
The Impact of Biocontrol Bacteria on Cotton Resistance and Their Effects on Signaling Pathways Related to Defense Against Verticillium Wilt Infection
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 12, 2024
Abstract
This
study
prepared
a
mixed
fermentation
broth
using
two
strains
of
Bacillus
and
investigated
its
inhibitory
effects
on
the
cotton
Verticillium
wilt
pathogen,
as
well
impact
signaling
pathways
related
to
defense
against
infection
in
cotton.Biocontrol
bacteria
can
effectively
defend
plant
diseases
by
competitively
inhibiting
pathogens
inducing
immunity.
Through
plate
confrontation
assays,
antimicrobial
tests
microbial
dilutions,
their
impacts
seed
germination,
this
explores
defensive
potential
broth.During
study,
it
was
discovered
that
The
could
produce
lipopeptide
substances.
cotton's
immunity
wilt,
following
treatment
with
broth,
assessed
DAB
trypan
blue
histological
staining
methods.
Furthermore,
involved
monitoring
induced
expression
resistance-related
genes
(PR1,
PR5,
NPR1),
activities
defense-related
enzymes
(SOD,
CAT,
PPO,
POD).The
results
indicate
combination
biocontrol
bacterial
exhibited
certain
effect
pathogen.
Root
drenching
significantly
enhanced
transient
burst
reactive
oxygen
species
pathways,
an
immune
response.
response
increased
sensitivity
hypersensitive
(HR),
disease
genes,
heightened
activity
scavenging,
thereby
enhancing
systemic
acquired
resistance
(SAR)
cotton.
reveals
improved
affecting
varying
different
genotypes
Язык: Английский
Evaluación in vitro y QSAR (Quantitative and Structure-Activity Relationship) de la actividad antifúngica de terpenoides obtenidos de aceites esenciales frente a Alternaria alternata y Fusarium oxysporum
Biomédica,
Год журнала:
2023,
Номер
43(Sp. 1), С. 156 - 169
Опубликована: Авг. 31, 2023
Fungal
genera
Alternaria
and
Fusarium
include
human
plant
pathogenic
species.
Several
antifungals
have
been
used
for
their
control,
but
excessive
use
has
contributed
to
resistance
development
in
pathogens.
An
alternative
searching
developing
new
antifungal
agents
is
using
essential
oils
main
components,
which
biological
activities
of
interest
medicine
food
production.To
evaluate
vitro
silico
the
terpenoids
against
alternata
oxysporum.The
minimum
inhibitory
concentration
fungicidal
values
27
constituents
oxysporum
were
evaluated
vitro.
In
addition,
genetic
algorithms,
quantitative
models
structure-activity
relationship
identify
structural
physicochemical
properties
related
activity.The
compounds
proved
be
effective
antifungals.
Thymol
was
most
active
with
a
91.6
±
28.8
μg/ml
A.
F.
oxysporum.
Quantitative
revealed
octanolwater
cleavage
ratio
as
molecular
property,
phenols
functional
group
contributing
activity.Terpenoids
exhibit
relevant
that
should
incorporated
into
study
medicinal
chemistry.
Inclusion
assays
evaluation
valuable
tool
search
rational
design
terpene
derivatives
potential
agents.Los
géneros
y
contienen
especies
patógenas
para
los
humanos
cultivos.
Para
su
se
han
utilizado
diversos
antifúngicos.
Sin
embargo,
uso
desmedido
ha
contribuido
al
desarrollo
de
agentes
patógenos
resistentes.
Una
alternativa
buscar
desarrollar
nuevos
antimicóticos
son
aceites
esenciales
sus
componentes
principales,
cuales
poseen
diversas
actividades
biológicas
interés
la
medicina
en
preservación
alimentos.Evaluar
e
las
antifúngicas
terpenoides
contra
oxysporum.Se
evaluaron
concentraciones
inhibitorias
mínimas
fungicidas
constituyentes
Además,
mediante
algoritmos
genéticos,
crearon
modelos
cuantitativos
relación
estructura-actividad
determinar
propiedades
estructurales
fisicoquímicas
relacionadas
con
actividad
antifúngica.Los
compuestos
evaluados
mostraron
ser
antifúngicos
activos.
El
timol
fue
el
compuesto
mayor
actividad,
un
valor
concentración
inhibitoria
mínima
μg/ml,
tanto
como
Los
incluyeron
avidez
por
lípidos
fenoles
principales
grupos
funcionales
que
contribuyen
relevantes
incorporados
estudio
química
medicinal.
La
inclusión
pruebas
evaluación
es
una
herramienta
útil
búsqueda
diseño
racional
derivados
terpénicos
posibles