Biocatalytic Efficient and Enantiocomplementary Synthesis of 3-Hydroxy-3-hydroxymethyloxindoles by Combining Halohydrin Dehalogenase and Epoxide Hydrolase

ACS Catalysis, Год журнала: 2025, Номер unknown, С. 992 - 1001

Опубликована: Янв. 2, 2025

Язык: Английский

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Biocatalytic enantioselective formation and ring-opening of oxetanes

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 30, 2025

Although biocatalysis offers complementary or alternative approaches to traditional synthetic methods, the limited range of available enzymatic reactions currently poses challenges in synthesizing a diverse array desired compounds. Consequently, there is significant demand for developing novel biocatalytic processes enable that were previously unattainable. Herein, we report discovery and subsequent protein engineering unique halohydrin dehalogenase develop platform enantioselective formation ring-opening oxetanes. This platform, exhibiting high efficiency, excellent enantioselectivity, broad scopes, facilitates preparative-scale synthesis chiral oxetanes variety γ-substituted alcohols. Additionally, both oxetane are proven scalable large-scale transformations at substrate concentrations, can be integrated efficiently one-pot, one-catalyst cascade system. work expands toolbox non-natural will promote further exploration catalytic repertoire dehalogenases pharmaceutical chemistry. Oxetane four-membered, oxygen-containing heterocyclic compound importance medicinal chemistry drug development. Here, authors dehalogenase,

Язык: Английский

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Characterization of the phenotype and functional alternations of three HCN1 variants in Chinese epilepsy patients

Epilepsy Research, Год журнала: 2025, Номер 211, С. 107521 - 107521

Опубликована: Фев. 7, 2025

Язык: Английский

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Semi-rational engineering of a novel halohydrin dehalogenase from Sneathiella limimaris for the enantioselective synthesis of (S)-5-phenoxymethyl-2-oxazolidinone

Bioorganic Chemistry, Год журнала: 2025, Номер 157, С. 108292 - 108292

Опубликована: Фев. 19, 2025

Язык: Английский

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