Rational design of a near-infrared fluorescent probe for rapid monitoring of carboxylesterase in live cells and drug-induced liver injury mice
W Xing,
Kaili Yang,
Yonglong Zhu
и другие.
Analytica Chimica Acta,
Год журнала:
2025,
Номер
1346, С. 343782 - 343782
Опубликована: Фев. 8, 2025
Язык: Английский
A comprehensive update on the application of high-throughput fluorescence imaging for novel drug discovery
Expert Opinion on Drug Discovery,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 30, 2025
High-throughput
fluorescence
imaging
(HTFI)
is
revolutionizing
drug
discovery
by
enabling
rapid
and
precise
detection
of
biological
targets
cellular
processes.
Recent
advances
in
technologies
now
provide
unprecedented
sensitivity,
resolution,
throughput.
Integration
artificial
intelligence
(AI)
machine
learning
(ML)
into
HTFI
workflows
significantly
enhances
data
processing,
aiding
hit
identification,
pattern
recognition,
mechanistic
understanding.
This
review
outlines
recent
technological
developments,
integration
strategies,
emerging
applications
HTFI.
It
emphasizes
HTFI's
role
phenotypic
screening,
especially
for
complex
diseases
such
as
cancer,
neurodegenerative
disorders,
viral
infections.
Additionally,
it
highlights
3D
culture
systems,
organoids,
organ-on-a-chip
technologies,
which
facilitate
physiologically
relevant
testing,
improved
predictive
accuracy,
translational
potential,
alongside
innovative
molecular
probes
biosensors.
Despite
its
advancements,
faces
ongoing
challenges,
including
standardization,
with
multi-omics
approaches,
scalability
advanced
models.
However,
progress
organoid
modeling
has
enhanced
the
physiological
relevance
assays,
complemented
sophisticated
AI
ML-driven
analysis
techniques.
Язык: Английский
Carboxylesterase-activated hepatocyte-targeting fluorescent probe for drug-induced liver injury diagnosis
Bioorganic Chemistry,
Год журнала:
2025,
Номер
unknown, С. 108587 - 108587
Опубликована: Май 1, 2025
Язык: Английский
An Innovative Aggregation-Induced Emission-Based NIR Fluorescent Probe for Visualizing Carboxylesterases in Living Cells, Zebrafish, and Tumor-Bearing Mice
Molecules,
Год журнала:
2024,
Номер
29(15), С. 3660 - 3660
Опубликована: Авг. 2, 2024
In
the
human
body,
carboxylesterases
(CEs)
play
crucial
roles
in
xenobiotic
metabolism
and
lipid
homeostasis.
But
abnormal
expression
of
CEs
is
highly
associated
with
some
diseases,
such
as
hyperlipidemia,
diabetes,
liver
cancer.
Therefore,
it
great
importance
to
develop
an
efficient
tool
for
accurate
detection
living
organisms.
Herein,
innovative
near-infrared
(NIR)
fluorescent
probe,
Язык: Английский
An Innovative Aggregation-Induced Emission-Based NIR Fluorescent Probe for Visualizing Carboxylesterases in Living Cells, Zebrafish and Tumor-Bearing Mice
Опубликована: Июнь 19, 2024
In
human
body,
carboxylesterases
(CEs)
play
the
crucial
roles
in
xenobiotic
metabolism
and
lipid
homeostasis.
But
abnormal
CEs
expression
is
highly
associated
with
some
diseases,
such
as
hyperlipidemia,
diabetes
liver
cancer.
Therefore,
it
of
great
significance
to
develop
an
efficient
tool
for
accurate
detection
living
organisms.
Herein,
innovative
near-infrared
(NIR)
fluorescent
probe
TTAP−AB
has
been
designed
based
on
aggregation-induced
emission
(AIE)
mechanism.
This
exhibits
rapid
response
(2
min),
excellent
sensitivity
(limit
=
8.14×10−6
U/mL),
high
selectivity
towards
CEs.
Additionally,
owing
good
biocompatibility,
enables
monitor
dynamic
changes
level
under
drug-induced
modulation
cells
zebrafish.
More
importantly,
successfully
employed
image
tumor
assist
resection
by
real-time
monitoring
CEs,
indicating
that
promising
guide
cancer
surgery.
can
not
only
enrich
strategies
biological
systems,
but
also
potential
clinical
imaging
applications,
including
medical
diagnosis,
preclinical
research,
imaging-guided
Язык: Английский