Rapid single-particle chemical imaging of nanoplastics by SRS microscopy

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(3)

Опубликована: Янв. 8, 2024

Plastics are now omnipresent in our daily lives. The existence of microplastics (1 µm to 5 mm length) and possibly even nanoplastics (<1 μm) has recently raised health concerns. In particular, believed be more toxic since their smaller size renders them much amenable, compared microplastics, enter the human body. However, detecting imposes tremendous analytical challenges on both nano-level sensitivity plastic-identifying specificity, leading a knowledge gap this mysterious nanoworld surrounding us. To address these challenges, we developed hyperspectral stimulated Raman scattering (SRS) imaging platform with an automated plastic identification algorithm that allows micro-nano analysis at single-particle level high chemical specificity throughput. We first validated enhancement narrow band SRS enable high-speed single nanoplastic detection below 100 nm. then devised data-driven spectral matching imposed by sensitive narrow-band achieve robust determination common polymers. With established technique, studied plastics from bottled water as model system. successfully detected identified major types. Micro-nano concentrations were estimated about 2.4 ± 1.3 × 10

Язык: Английский

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Polydiacetylene-based ultrastrong bioorthogonal Raman probes for targeted live-cell Raman imaging

Nature Communications, Год журнала: 2020, Номер 11(1)

Опубликована: Янв. 3, 2020

Abstract Live-cell Raman imaging based on bioorthogonal probes with distinct signals in the cellular Raman-silent region (1800–2800 cm −1 ) has attracted great interest recent years. We report here a class of water-soluble and biocompatible polydiacetylenes intrinsic ultrastrong alkyne that locate this for organelle-targeting live-cell imaging. Using host-guest topochemical polymerization strategy, we have synthesized functionalizable master polydiacetylene, namely poly(deca-4,6-diynedioic acid) (PDDA), which possesses significantly enhanced (up to ~10 4 fold) vibration compared conventional probes. In addition, PDDA can be used as general platform multi-functional achieve high quality stimulated scattering basis modified PDDA. The polydiacetylene-based represent agents (without any enhancer), flexible functionalization material holds promise its potential diverse applications.

Язык: Английский

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Stimulated Raman excited fluorescence spectroscopy and imaging

Nature Photonics, Год журнала: 2019, Номер 13(6), С. 412 - 417

Опубликована: Апрель 1, 2019

Язык: Английский

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Optical mapping of biological water in single live cells by stimulated Raman excited fluorescence microscopy

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Окт. 18, 2019

Water is arguably the most common and yet least understood material on Earth. Indeed, biophysical behavior of water in crowded intracellular milieu a long-debated issue. Understanding spatial compositional heterogeneity inside cells remains elusive, largely due to lack proper water-sensing tools with high sensitivity resolution. Recently, stimulated Raman excited fluorescence (SREF) microscopy was reported as sensitive vibrational imaging optical far field. Herein we develop SREF into tool by coupling it solvatochromism. This technique allows us directly visualize spatially-resolved distribution states single mammalian cells. Qualitatively, our result supports concept biological reveals between nucleus cytoplasm. Quantitatively, unveil map pool living Hence hope will be promising study its relationship cellular activities.

Язык: Английский

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Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities

Journal of the American Chemical Society, Год журнала: 2020, Номер 142(49), С. 20701 - 20707

Опубликована: Ноя. 23, 2020

Raman probes based on alkyne or nitrile tags hold promise for highly multiplexed imaging. However, sensing of enzyme activities with is difficult because few mechanisms are available to modulate the vibrational response. Here we present a general strategy prepare activatable that show enhanced signals due electronic preresonance (EPR) upon reaction enzymes under physiological conditions. We identified xanthene derivative bearing group at position 9 (9CN-JCP) as suitable scaffold dye, and synthesized four types probes, which targeted different (three aminopeptidases glycosidase) tuned frequencies by isotope editing group. validated activation these target succeeded in simultaneous imaging live cells. Different cell lines showed patterns activities.

Язык: Английский

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Highly-multiplexed volumetric mapping with Raman dye imaging and tissue clearing

Nature Biotechnology, Год журнала: 2021, Номер 40(3), С. 364 - 373

Опубликована: Окт. 4, 2021

Язык: Английский

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Ultra-bright Raman dots for multiplexed optical imaging

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Фев. 26, 2021

Abstract Imaging the spatial distribution of biomolecules is at core modern biology. The development fluorescence techniques has enabled researchers to investigate subcellular structures with nanometer precision. However, multiplexed imaging, i.e. observing complex biological networks and interactions, mainly limited by fundamental ‘spectral crowding’ fluorescent materials. Raman spectroscopy-based methods, on other hand, have a much greater spectral resolution, but often lack required sensitivity for practical imaging biomarkers. Addressing pressing need new probes, herein we present series Raman-active nanoparticles (Rdots) that exhibit combined advantages ultra-brightness compact sizes (~20 nm). When coupled emerging stimulated scattering (SRS) microscopy, these Rdots are brighter than previously reported organic probes two three orders magnitude. We further obtain evidence supporting SRS single particle level. size allows immunostaining specific protein targets (including cytoskeleton low-abundant surface proteins) in mammalian cells tissue slices high contrast. These thus offer promising tool large range studies networks.

Язык: Английский

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Super‐Resolution Vibrational Imaging Using Expansion Stimulated Raman Scattering Microscopy

Advanced Science, Год журнала: 2022, Номер 9(20)

Опубликована: Май 6, 2022

Abstract Stimulated Raman scattering (SRS) microscopy is an emerging technology that provides high chemical specificity for endogenous biomolecules and can circumvent common constraints of fluorescence including limited capabilities to probe small difficulty resolving many colors simultaneously. However, the resolution SRS remains governed by diffraction limit. To overcome this, a new technique called molecule anchorable gel‐enabled nanoscale Imaging Fluorescence stimulated (MAGNIFIERS) integrates with expansion (ExM) described. MAGNIFIERS offers chemical‐specific imaging sub‐50 nm has scalable multiplexity when combined multiplex probes fluorescent labels. used visualize features in label‐free manner CH vibration proteins, lipids, DNA broad range biological specimens, from mouse brain, liver, kidney human lung organoid. In addition, applied track protein synthesis aggregates using metabolic labeling metabolites. Finally, demonstrate 8‐color expanded brain section. Overall, valuable platform super‐resolution imaging, high‐resolution highly multiplexed thus bringing nanoscopy.

Язык: Английский

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Stimulated Raman photothermal microscopy toward ultrasensitive chemical imaging

Science Advances, Год журнала: 2023, Номер 9(43)

Опубликована: Окт. 27, 2023

Stimulated Raman scattering (SRS) microscopy has shown enormous potential in revealing molecular structures, dynamics, and couplings complex systems. However, the sensitivity of SRS is fundamentally limited to millimolar level due shot noise small modulation depth. To overcome this barrier, we revisit from perspective energy deposition. The process pumps molecules their vibrationally excited states. subsequent relaxation heats up surroundings induces refractive index changes. By probing changes with a laser beam, introduce stimulated photothermal (SRP) microscopy, where >500-fold boost depth achieved. versatile applications SRP on viral particles, cells, tissues are demonstrated. opens way perform vibrational spectroscopic imaging ultrahigh sensitivity.

Язык: Английский

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Computational coherent Raman scattering imaging: breaking physical barriers by fusion of advanced instrumentation and data science

eLight, Год журнала: 2023, Номер 3(1)

Опубликована: Март 20, 2023

Abstract Coherent Raman scattering (CRS) microscopy is a chemical imaging modality that provides contrast based on intrinsic biomolecular vibrations. To date, endeavors instrumentation have advanced CRS into powerful analytical tool for studies of cell functions and in situ clinical diagnosis. Nevertheless, the small cross-section sets up physical boundary design space system, which trades off speed, signal fidelity spectral bandwidth. The synergistic combination computational approaches offers way to break trade-off. In this review, we first introduce coherent recent developments, then discuss current methods, including compressive micro-spectroscopy, volumetric imaging, as well machine learning algorithms improve system performance decipher information. We foresee constant permeation concepts push capability microscopy.

Язык: Английский

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