bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 16, 2024
Pulmonary
drug
delivery
is
critical
to
the
treatment
of
respiratory
diseases.
However,
human
airway
surface
presents
multiscale
barriers
efficient
delivery.
Here
we
report
a
bottlebrush
polyethylene
glycol
(PEG-BB)
nanocarrier
that
can
translocate
across
all
within
surface.
Guided
by
molecular
theory,
design
PEG-BB
molecule
consisting
linear
backbone
densely
grafted
many
(∼1,000)
low
weight
(∼1000
g/mol)
PEG
chains;
this
results
in
highly
anisotropic,
wormlike
featuring
contour
length
∼250
nm,
cross-section
∼20
and
hydrodynamic
diameter
∼40
nm.
Using
classic
air-liquid-interface
culture
system
recapitulate
essential
biological
features
surface,
show
rapidly
penetrates
through
endogenous
mucus
periciliary
brush
layer
(mesh
size
20-40
nm)
be
internalized
cells
whole
epithelium.
By
quantifying
cellular
uptake
polymeric
carriers
various
architectures
manipulating
cell
proliferation
endocytosis
pathways,
translocation
epithelium
driven
architecture
enhanced
endocytosis.
Our
demonstrate
large,
polymers,
if
properly
designed,
used
as
novel
carrier
for
pulmonary
mucosal
Polymers,
Год журнала:
2024,
Номер
16(18), С. 2629 - 2629
Опубликована: Сен. 18, 2024
Gene
therapy
is
the
technique
of
inserting
foreign
genetic
elements
into
host
cells
to
achieve
a
therapeutic
effect.
Although
gene
was
initially
formulated
as
potential
remedy
for
specific
problems,
it
currently
offers
solutions
many
diseases
with
varying
inheritance
patterns
and
acquired
diseases.
There
are
two
major
groups
vectors
therapy:
viral
vector
non-viral
therapy.
This
review
examines
role
macromolecule's
chemical
physical
architecture
in
delivery,
including
their
design
synthesis.
Polymers
can
boost
circulation,
improve
control
cargo
release
through
various
methods.
The
prominent
examples
discussed
include
poly-L-lysine,
polyethyleneimine,
comb
polymers,
brush
star
well
hydrogels
natural
polymers
modifications.
While
significant
progress
has
been
made,
challenges
still
exist
stabilization,
targeting
specificity,
cellular
uptake.
Overcoming
cytotoxicity,
improving
delivery
efficiency,
utilizing
hybrid
systems
vital
factors
prospects.
comprehensive
provides
an
illuminating
overview
field,
guiding
way
toward
innovative
non-viral-based
solutions.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 27, 2024
Abstract
Currently,
many
types
of
non-linear
topological
structure
polymers,
such
as
brush-shaped,
star,
branched
and
dendritic
structures,
have
captured
much
attention
in
the
field
gene
delivery
nanomedicine.
Compared
with
linear
structural
polymers
offer
advantages,
including
multiple
terminal
groups,
broad
complicated
spatial
architecture
multi-functionality
sites
to
enhance
efficiency
targeting
capabilities.
Nevertheless,
complexity
their
synthesis
process
severely
hampers
development
applications
nonlinear
polymers.
This
review
aims
highlight
various
synthetic
approaches
reversible-deactivation
radical
polymerization
(RDRP)
atom-transfer
(ATRP),
nitroxide-mediated
(NMP),
reversible
addition-fragmentation
chain
transfer
(RAFT)
polymerization,
click
chemistry
reactions
Michael
addition,
thoroughly
discuss
advantages
disadvantages,
well
analyze
further
application
potential.
Finally,
we
comprehensively
summarize
different
for
genetic
materials
delivering
performance
both
vitro
vivo,
which
indicated
that
effects
topologies
play
a
crucial
role
enhancing
transfection
polymeric
vectors.
offered
promising
guideline
design
novel
facilitated
new
generation
polymer-based
Macromolecules,
Год журнала:
2024,
Номер
57(11), С. 5368 - 5379
Опубликована: Май 29, 2024
Topology
significantly
impacts
polymer
properties
and
applications.
Hyperbranched
polymers
(HBPs)
synthesized
via
atom
transfer
radical
polymerization
(ATRP)
using
inimers
typically
exhibit
broad
molecular
weight
distributions
limited
control
over
branching.
Alternatively,
copolymerization
of
inibramers
(IB),
such
as
α-chloro/bromo
acrylates
with
vinyl
monomers,
yields
HBPs
precise
uniform
Herein,
we
described
the
synthesis
hydrophilic
HB
polyacrylates
in
water
by
copolymerizing
a
water-soluble
IB,
oligo(ethylene
oxide)
methyl
ether
2-bromoacrylate
(OEOBA),
various
acrylate
comonomers.
Visible-light-mediated
controlled
branching
(CRBP)
dual
catalysis
eosin
Y
(EY)
copper
complexes
resulted
weights
(Mn
=
38
000
to
170
000)
degrees
(2%–24%).
Furthermore,
optimized
conditions
enabled
successful
application
OEOBA
synthesize
linear-hyperbranched
block
copolymers
hyperbranched
protein
hybrids
(HB-PPH),
demonstrating
its
potential
advance
complex
macromolecular
architecture
under
environmentally
benign
conditions.
Copolymerization
methacrylate
monomer,
(OEOMA500),
inibramer
was
accompanied
fragmentation
β-carbon
C–C
bond
scission
subsequent
growth
chains
from
fragments.
computational
studies
investigating
depending
on
IB
comonomer
structure
supported
experimental
observations.
This
work
expands
toolkit
for
CRBP
highlights
critical
influence
reaction
outcomes.
Materials Horizons,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Magnetic
resonance
imaging
(MRI)
is
considered
one
of
the
most
valuable
diagnostic
technologies
in
21st
century.
To
enhance
image
contrast
anatomical
features,
MRI
agents
have
been
widely
used
clinical
diagnosis,
especially
those
based
on
gadolinium,
manganese,
and
iron
oxide.
However,
these
metal-based
show
potential
toxicity
to
patients,
which
urges
researchers
develop
novel
that
can
replace
agents.
Metal-free
nitroxide
radical
(NRCAs)
effectively
overcome
shortcomings
also
many
advantages,
including
good
biocompatibility,
prolonged
systemic
circulation
time,
easily
functionalized
structures.
Importantly,
since
NRCAs
acquire
signals
with
standard
tissue
water
ACS Polymers Au,
Год журнала:
2024,
Номер
4(2), С. 98 - 108
Опубликована: Янв. 4, 2024
Three-dimensional
(3D)
printing
of
elastomers
enables
the
fabrication
many
technologically
important
structures
and
devices.
However,
there
remains
a
critical
need
for
development
reprocessable,
solvent-free,
soft
that
can
be
printed
without
post-treatment.
Herein,
we
report
modular
suitable
direct
ink
writing
(DIW)
by
physically
cross-linking
associative
polymers
with
high
fraction
reversible
bonds.
We
designed
synthesized
linear-associative-linear
(LAL)
triblock
copolymers;
middle
block
is
an
polymer
carrying
amide
groups
form
double
hydrogen
bonding,
end
blocks
aggregate
to
hard
glassy
domains
effectively
act
as
physical
cross-links.
The
do
not
nanoscale
clusters
only
slow
down
dynamics
changing
shape
linear
viscoelastic
spectra;
this
molecular
control
over
energy
dissipation
varying
groups.
Increasing
volume
increases
network
stiffness
more
than
100
times
significantly
compromising
extensibility.
created
Young's
moduli
ranging
from
8
kPa
MPa
while
maintaining
tensile
breaking
strain
around
150%.
Using
high-temperature
DIW
platform,
transformed
our
complex,
highly
deformable
3D
involving
any
solvent
or
post-print
processing.
Our
represent
softest
melt
reprocessable
materials
printing.
developed
LAL
synergize
emerging
homogeneous
bonds
classical
copolymer
self-assembly
dual-cross-linked
network,
providing
versatile
platform
design
elastomeric
practical
applications.
Since
the
invention
of
polymer
networks
such
as
cross-linked
natural
rubber
in
19th
century,
it
has
been
a
dogma
that
stiffer
are
less
stretchable.
We
report
universal
strategy
for
decoupling
stiffness
and
extensibility
single-network
elastomers.
Instead
using
linear
polymers
network
strands,
we
use
foldable
bottlebrush
polymers,
which
feature
collapsed
backbone
grafted
with
many
side
chains.
Upon
elongation,
unfolds
to
release
stored
length,
enabling
remarkable
extensibility.
By
contrast,
elastic
modulus
is
inversely
proportional
strand
mass
determined
by
validate
this
concept
creating
elastomers
nearly
constant
Young’s
(30
kilopascals)
while
increasing
tensile
breaking
strain
40-fold,
from
20
800%.
show
applies
different
species
topologies.
Our
discovery
opens
an
avenue
developing
polymeric
materials
extraordinary
mechanical
properties.
ACS Macro Letters,
Год журнала:
2023,
Номер
12(8), С. 1025 - 1030
Опубликована: Июль 11, 2023
Cyclic
polymers
with
cleavable
backbones
triggered
by
either
external
or
internal
stimuli
can
realize
simultaneous
extracellular
stability
and
intracellular
destabilization
of
cyclic
polymer-based
nanocarriers
but
remain
seldom
reported.
To
this
end,
we
prepared
herein
cyclic-ONB-P(OEGMA-st-DMAEMA)
(c-ONB-P(OEGMA-st-DMAEMA))
a
light-cleavable
junction
in
the
polymer
backbone
based
on
oligo
(ethylene
glycol)
monomethyl
ether
methacrylate
(OEGMA)
N,N-dimethylaminoethyl
(DMAEMA)
using
atom
transfer
radical
polymerization
(ATRP)
initiator
containing
an
o-nitrobenzyl
(ONB)
ester
group.
Together
pH-sensitivity
DMAEMA,
c-ONB-P(OEGMA-st-DMAEMA)
shows
mainchain
pH-sensitive
side
chains.
Notably,
doxorubicin
(DOX)-loaded
c-ONB-P(OEGMA4-st-DMAEMA38)
(C2)
micelles
mediated
IC50
value
2.28
μg/mL
Bel-7402
cells,
which
is
1.7-fold
lower
than
that
acquired
without
UV
irradiation.
This
study
thus
reported
synthesis
copolymer
UV-cleavable
uncovered
effects
topological
modulation
vitro
controlled
release
properties
polymers.
ACS Nano,
Год журнала:
2024,
Номер
18(27), С. 17586 - 17599
Опубликована: Июнь 27, 2024
Pulmonary
drug
delivery
is
critical
for
the
treatment
of
respiratory
diseases.
However,
human
airway
surface
presents
multiple
barriers
to
efficient
delivery.
Here,
we
report
a
bottlebrush
poly(ethylene
glycol)
(PEG-BB)
nanocarrier
that
can
translocate
across
all
within
surface.
Guided
by
molecular
theory,
design
PEG-BB
molecule
consisting
linear
backbone
densely
grafted
many
(∼1000)
low
weight
(∼1000
g/mol)
polyethylene
glycol
(PEG)
chains;
this
results
in
highly
anisotropic,
wormlike
featuring
contour
length
∼250
nm,
cross-section
∼20
and
hydrodynamic
diameter
∼40
nm.
Using
classic
air-liquid-interface
culture
system
recapitulate
essential
biological
features
surface,
show
rapidly
penetrates
through
endogenous
mucus
periciliary
brush
layer
(mesh
size
20-40
nm)
be
internalized
cells
whole
epithelium.
By
quantifying
cellular
uptake
polymeric
carriers
various
architectures
manipulating
cell
proliferation
endocytosis
pathways,
translocation
epithelium
driven
architecture-enhanced
endocytosis.
Our
demonstrate
large,
PEG
polymers,
if
properly
designed,
used
as
carrier
pulmonary
mucosal
