ACS Omega,
Год журнала:
2024,
Номер
9(22), С. 24050 - 24059
Опубликована: Май 19, 2024
Glass
nanopipettes,
as
important
sensing
tools,
have
attracted
great
interest
due
to
their
wide
range
of
applications
in
detecting
single
molecules,
nanoparticles,
and
cells.
In
this
study,
we
investigated
the
translocation
behavior
nanorod
particles
through
dual-nanopipettes
using
a
transient
continuum-based
model
based
on
an
arbitrary
Lagrangian-Eulerian
approach.
Our
findings
indicate
that
nanorods
is
slowed
down
dual-nanopipette
system,
especially
system
with
nanobridge.
These
results
are
qualitative
agreement
previous
experimental
reported
literature.
Additionally,
influenced
by
factors
such
bulk
concentration,
initial
location
nanorod,
surface
charge
nanopipette.
Notably,
when
density
nanopipette
relatively
high
reservoir,
can
hardly
enter
nanopipette,
resulting
low
efficiency.
However,
efficiency
be
improved
initially
positioning
one
barrels.
The
dual-blockade
current
signal
used
correlate
characteristics
nanorod.
Nano Letters,
Год журнала:
2023,
Номер
24(1), С. 202 - 208
Опубликована: Дек. 21, 2023
This
work
presents
a
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/Cas-nanopipette
nano-electrochemistry
(Cas
=
CRISPR-associated
proteins)
capable
of
ultrasensitive
microRNA
detection.
Nanoconfinement
the
CRISPR/Cas13a
within
nanopipette
leads
to
high
catalytic
efficacy
ca.
169
times
higher
than
that
in
bulk
electrolyte,
contributing
amplified
electrochemical
responses.
CRISPR/Cas13a-enabled
detection
representative
microRNA-25
achieves
low
limit
down
10
aM.
Practical
application
this
method
is
further
demonstrated
for
single-cell
and
real
human
serum
Its
general
applicability
validated
by
addressing
microRNA-141
SARS-CoV-2
RNA
gene
fragment.
introduces
new
CRISPR/Cas-empowered
nanotechnology
bioanalysis.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(16), С. 6444 - 6449
Опубликована: Апрель 10, 2024
As
two
mainstream
ionic
detection
techniques,
current
rectification
(ICR)
suffers
from
large
fluctuations
in
trace
level
detection,
while
resistive-pulse
sensing
(RPS)
encounters
easy
clogs
high-concentration
detection.
By
rationally
matching
the
nanopore
size
with
DNA
tetrahedron
(TDN),
this
work
bridges
techniques
to
achieve
reliable
wide
linearity.
a
representative
analyte,
miRNA-10b
could
specifically
combine
and
release
TDN
interior
wall,
which
thus
induced
simultaneous
generation
of
distinct
ICR
RPS
signals.
The
signals
be
attributed
balance
between
effective
orifice
surface
charge
density
inner
were
by
complex
passing
through
nanopore.
Such
an
operation
contributed
range
1
fM-1
nM
good
feasibility
method
is
also
validated
single-cell
real
plasma
ACS Nano,
Год журнала:
2024,
Номер
18(5), С. 4551 - 4558
Опубликована: Янв. 24, 2024
Developing
nanoscale
ratiometric
techniques
capable
of
biochemical
response
should
prove
significance
for
precise
applications
with
stringent
spatial
and
biological
restrictions.
Here
we
present
devise
the
concept
θ-nanopore
ratiometry,
which
uses
signals
that
could
well
address
serious
concerns
about
device
deviation
in
fabrication
nonspecific
adsorption
detection.
As
exemplified
by
a
200
nm
toward
miRNA
detection,
±20
aperture
drift
be
mitigated
issue
minimized
complex
cytosolic
environment.
Practical
application
this
ratiometry
realizes
measurements
miRNA-10b.
This
work
has
not
only
established
nanoscopic
technique
but
also
enriched
extant
armory
nanotools
single-cell
studies
beyond.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 16, 2025
A
novel
biosensing
platform
integrating
molecular
gating
mechanisms
with
confined-space
amplification
is
presented
for
ultrasensitive
nucleic
acid
detection.
The
employs
a
target-triggered
clamped
hybridization
chain
reaction
(C-HCR)
to
form
DNA
hydrogel
networks
in
gold-modified
glass
micropipettes,
creating
nanoconfined
spaces.
Notably,
this
configuration
demonstrates
effective
ion
enhanced
sensitivity
through
the
nanoconfinement
effect
as
modulation
of
current
rectification
signals
significantly
amplified.
Moreover,
can
be
regenerated
thermal
treatment,
endowing
device
good
recoverability.
performance
was
validated
by
using
different
antibiotic
resistance
genes
model
targets.
results
showed
high
specificity
and
limits
detection
down
subnanomolar
levels.
confined
environment
enables
sensitive
small
sample
volumes
only
4
μL.
strategy
leveraging
amplify
signal
transduction
may
provide
inspiration
rational
design
other
high-performance
biosensors,
potentially
holding
great
promise
disease
diagnostics
environmental
monitoring.
The
analysis
of
tumor
metastasis-associated
proteins
is
vital
importance
for
monitoring
evolution
and
evaluating
therapeutic
outcomes
protein-targeted
drugs.
However,
electrochemically
analyzing
both
the
abundance
activity
these
inactive
in
single
living
cells
remains
an
unresolved
challenge.
Here,
we
develop
electrochemical
sensor
composed
a
nanoelectrode
functionalized
by
atomically
precise
metal
clusters
with
excellent
electrocatalytic
activity.
It
enables
highly
selective
detection
associated
metastasis.
Via
this
sensor,
demonstrate
relation
between
cathepsin
B
metastatic
features
cells.
inherent
heterogeneous
protein
expression
among
individual
confirmed
quantitative
manner.
Additionally,
methodology
allows
assessment
response
to
antimetastatic
drug
exposure
at
single-cell
level.
deepens
our
understanding
B's
role
metastasis
provides
potential
evaluation
B-targeted
Analytical Chemistry,
Год журнала:
2024,
Номер
96(10), С. 4190 - 4196
Опубликована: Фев. 27, 2024
Dual
nanopipettes
with
two
channels
have
been
receiving
great
attention
due
to
the
convenient
experimental
setup
and
multiple
measuring
in
sensing
applications
at
nanoscale,
while
involved
dynamic
asymmetrical
ion
transport
processes
not
fully
elucidated.
In
this
paper,
both
simulation
methods
are
used
investigate
mass
inside
dual
well-separated
channels.
The
results
present
that
resistance
through
(R12)
is
always
add-up
of
individual
ones
(R13
+
R23)
respect
bulk
solutions,
various
ionic
strengths
scan
rates.
A
constant
zero-current
potential
obtained
when
loading
an
electrolyte
concentration
channels,
zero-potential
current
displays
a
good
linear
relationship
outside
pipet.
Besides
revealing
polarization
nanopipettes,
these
would
also
further
promote
better
usage
electrochemical
imaging
applications.
Chemical Communications,
Год журнала:
2023,
Номер
59(97), С. 14463 - 14466
Опубликована: Янв. 1, 2023
We
report
here
a
dual-nanopore
biosensor
based
on
modulation
of
surface
charge
density
coupled
with
microwell
array
chip
for
in
situ
monitoring
ROS
secretion
from
single
MCF-7
cells.
