International Immunopharmacology,
Год журнала:
2024,
Номер
137, С. 112488 - 112488
Опубликована: Июнь 17, 2024
Monomethyl
fumarate
(MMF),
a
potent
anti-inflammatory
agent
used
to
treat
multiple
sclerosis,
has
demonstrated
efficacy
in
various
inflammatory
and
ischemia/reperfusion
(IR)
models;
however,
its
impact
on
IR-induced
acute
lung
injury
(ALI)
not
been
explored.
We
investigated,
for
the
first
time,
whether
MMF
attenuates
IR
through
inhibition
of
GAPDH/Siah1
signaling
pathway.
Rats
were
subjected
using
an
isolated
perfused
model,
proximity
ligation
assays
employed
evaluate
presence
distribution
complex.
In
vitro
studies
involved
pretreating
human
primary
alveolar
epithelial
cells
(HPAECs)
with
and/or
inducing
GAPDH
overexpression
or
silencing,
followed
by
exposure
hypoxia-reoxygenation.
The
findings
revealed
significantly
reduced
damage
indicators,
including
edema,
proinflammatory
cytokines,
oxidative
stress
apoptosis,
MMF-treated
rats.
Notably,
treatment
inhibited
complex
formation
nuclear
translocation,
indicating
that
disruption
cascade
was
cause
these
improvements.
Our
pretreated
HPAECs
corroborate
vivo
findings,
further
strengthening
this
interpretation.
study
results
suggest
protective
effects
against
may
be
attributed,
at
least
part,
ability
disrupt
cascade,
thereby
attenuating
apoptotic
responses.
Given
encouraging
results,
emerged
as
promising
therapeutic
candidate
management
injury.
ACS Applied Bio Materials,
Год журнала:
2024,
Номер
7(4), С. 2175 - 2185
Опубликована: Март 13, 2024
Lung
cancer
and
Mycobacterium
avium
complex
infection
are
lung
diseases
associated
with
high
incidence
mortality
rates.
Most
conventional
anticancer
drugs
antibiotics
have
certain
limitations,
including
drug
resistance
rates
adverse
effects.
Herein,
we
aimed
to
synthesize
mannose
surface-modified
solid
lipid
nanoparticles
(SLNs)
loaded
curcumin
(Man-CUR
SLN)
for
the
effective
treatment
of
disease.
The
synthesized
Man-CUR
SLNs
were
analyzed
using
various
instrumental
techniques
structural
physicochemical
characterization.
Loading
into
improved
encapsulation
efficiency
release
capacity,
as
demonstrated
by
high-performance
liquid
chromatography
analysis.
Furthermore,
characterized
effect
A549
cell
line.
Cells
treated
SLN
exhibited
an
increased
cellular
uptake
cytotoxicity.
Moreover,
free
CUR
could
more
effectively
reduce
migration
than
SLNs.
Similarly,
elicited
a
stronger
apoptosis-inducing
that
SLNs,
reverse
transcription-quantitative
PCR
Finally,
examined
antibacterial
effects
against
intracellulare
(M.i.)
M.i.-infected
macrophages,
revealing
exerted
strongest
effect.
Collectively,
these
findings
indicate
mannose-receptor-targeted
delivery
be
in
treating
diseases.
Accordingly,
this
system
can
used
target
variety
cancers
immune
cells.
ACS Nano,
Год журнала:
2024,
Номер
18(41), С. 28228 - 28245
Опубликована: Окт. 5, 2024
Sepsis-associated
encephalopathy
(SAE)
is
a
devastating
complication
of
sepsis,
affecting
approximately
70%
patients
with
sepsis
in
intensive
care
units
(ICU).
Although
the
pathophysiological
mechanisms
remain
elusive,
typically
accompanied
by
systemic
inflammatory
response
syndrome
(SIRS)
and
hyper-oxidative
conditions.
Here,
we
introduce
biomimetic
nanomodulator
(mAOI
NP)
that
specifically
targets
inflammation
site
simultaneously
regulates
oxidative
stresses.
mAOI
NPs
are
constructed
using
metal-coordinated
polyphenolic
antioxidants
(tannic
acid)
flavonoid
quercetin,
which
then
coated
macrophage
membrane
to
enhance
pharmacokinetics
enable
SAE
targeting.
In
cecal
ligation
puncture
(CLP)-induced
severe
model,
effectively
mitigate
stress
purging
reactive
oxygen
species,
repairing
mitochondrial
damage
activating
Nrf2/HO-1
signaling
pathway;
while
polarizing
M1
macrophages
or
microglia
toward
anti-inflammatory
M2
subtype.
potently
inhibit
progress,
prolong
overall
survival
from
25
66%
learning
memory
capabilities
mice.
Further
proteomics
analysis
reveals
modulate
neurodevelopment
processes
related
formation
also
exerting
antioxidative
effects
on
brain
tissue
responses
associated
pathology.
This
study
offers
significant
potential
for
improving
patient
outcomes
revolutionizing
treatment
landscape
this
sepsis.
Journal of Advanced Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 1, 2025
Acute
lung
injury
(ALI)
is
a
life-threatening
condition
characterized
by
rapidly
progressing
respiratory
distress
and
hypoxemia.
Oxidative
stress-induced
inflammation
in
tissue
plays
crucial
role
the
progression
of
ALI.
Excessive
generation
reactive
oxygen
species
(ROS)
pulmonary
microenvironment
activates
inflammatory
signaling
pathways,
enhancing
transcription
pro-inflammatory
factors
ultimately
leading
to
necrosis.
Bilirubin
(BR),
an
exceptional
endogenous
antioxidant,
possesses
ability
counteract
elevated
levels
through
direct
reactions
or
inducing
antioxidant
systems
such
as
Nrf2/HO-1
signaling.
However,
its
limited
solubility
poses
hindrance
further
applications.
Hence,
it
imperative
develop
suitable
bilirubin-based
system
for
biological
utilization.
In
this
study,
we
developed
ROS-sensitive
adaptive
nanoscavenger
(GP@BR)
co-assembling
bilirubin-conjugated
glycol
chitosan
(GC-BR)
polyethylene
(PEG-BR),
aiming
alleviate
oxidative
stress
ALI
treatment.
The
different
conjugations
endowed
bilirubin
derivatives
with
varying
sensitivity
towards
reacting
ROS,
enabling
GP@BR
exert
antioxidative
properties
specifically
environments
on
demand.
Besides
excellent
properties,
also
demonstrated
absorb
excess
cytokines.
Moreover,
our
optimized
facilitated
transport
across
mucosal
layer
epithelial
cells.
vivo
studies
confirmed
that
significantly
improved
symptoms
suppressed
fibrosis.
This
study
highlighted
potential
multiple
actions
treatment
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 8, 2025
Abstract
The
hypoxic
microenvironment
and
radioresistance
of
tumor
cells,
as
well
the
delay
in
efficacy
evaluation,
significantly
limit
effect
clinical
radiotherapy.
Therefore,
developing
effective
radiosensitizers
with
monitoring
response
is
great
significance
for
precise
Herein,
a
novel
radiosensitizer
(term
as:
SCuFs)
developed,
consisting
traditional
Chinese
medicine
(TCM)
compounds
salidroside,
Cu
2+
,
hydroxyl
radical
(•OH)
activated
second
near‐infrared
window
fluorescence
(NIR‐II
FL)
molecules,
which
make
radiosensitization
boosted
chemodynamic
therapy
(CDT)
efficacy.
overexpressed
glutathione
induces
SCuFs
dissociation,
allowing
deep
penetration
drug
to
whole
region.
After
X‐ray
irradiation,
salidroside
inhibits
Nuclear
factor
erythroid
2‐like
2
(Nrf2)protein
expression
blocks
cells
G2/M
phase
highest
radiosensitivity,
amplifies
reactive
oxygen
species
(ROS)
generation
exacerbate
DNA
damage,
thus
achieving
radiosensitization.
Meanwhile,
upregulated
ROS
provides
sufficient
chemical
fuel
+
‐mediated
CDT
produce
more
•OH.
NIR‐II
FL
imaging
can
monitor
•OH
changes
during
process,
confirming
process
related
This
study
not
only
achieves
cascaded
ROS‐mediated
efficacy,
but
also
useful
tool
therapeutic
showing
prospects
application.
