ACS Applied Materials & Interfaces, Год журнала: 2024, Номер unknown

Опубликована: Дек. 11, 2024

In the existing development of extensive drug screening models, 3D cell cultures outshine conventional 2D monolayer cells by closely imitating in vivo tumor microenvironment. This makes culture a more physiologically relevant and convenient system regime preclinical testing. nanomedicinal world, nanoconjugates as nanocarriers are largely hunted due to their capability precisely binding target distributing essential dosages therapeutic drugs with enhanced safety profiles. Thus, for boosted availability, evolution from treatment combination therapies last switching carriers has gained significant progression cancer cure. contrast engineered nanoparticles, herein, we successfully designed biomolecule (ferritin)-based effective both single (valproic acid-VPA) twin-drug acid/doxorubicin-Dox) carriers, which dramatically enhance proficiency modality. To question reported adjuvant property VPA, progressed utilizing at first VPA alone an yet exclusive therapy when delivered via some carrier molecule, particular protein. Subsequently, paralleled this comprehensive investigation output compare test coloading strategy observe synergistic and/or additive behavior conjugation other anticancer agents (Dox) while given molecule. approach this, Dox molecules were encapsulated into ferritin (F) cavity using thermosensitive synthesis method maintaining temperature 60 °C. The successful encapsulation protein nanocage was confirmed through various characterization techniques. F-VPA/F-VPA-Dox exhibited similar morphology structural characteristics hollow cage showed cytotoxicity than naked tested on spheroid models. Precisely, our nanoconjugate, i.e., F-VPA, offered 3-fold increased intratumoral concentration free significantly suppressed growth after single-dose treatment. However, second modeled viz. F-VPA-Dox, revealed extended median survival period lesser toxicity administered much dose (∼3–5 μM), models lines. All all, importantly, inhibition rate treatment, further confirms benefits active targeting these nanocarriers. Moreover, also offer deliver cells, alongside tremendous biocompatibility profiles numerous

Язык: Английский