Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
13
Опубликована: Март 28, 2025
The
management
of
bone
defects,
particularly
in
aging
populations,
remains
a
major
clinical
challenge.
immune
microenvironment
plays
an
important
role
the
repair
defects
and
favorable
environment
can
effectively
promote
defects.
However,
is
closely
associated
with
chronic
low-grade
systemic
inflammation,
which
adversely
affects
healing.
Persistent
inflammation
critically
regulates
through
all
stages.
This
review
explores
potential
3D-printed
bioceramic
scaffolds
defect
repair,
focusing
on
their
capacity
to
modulate
counteract
effects
aging.
not
only
provide
structural
support
for
regeneration
but
also
serve
as
effective
carriers
anti-osteoporosis
drugs,
offering
novel
therapeutic
strategy
treating
osteoporotic
By
regulating
improving
response,
may
significantly
enhance
context
age-related
degeneration.
approach
underscores
advanced
biomaterials
addressing
dual
challenges
dysregulation,
promising
avenues
development
treatments
elderly.
We
hope
concepts
discussed
this
could
offer
strategies
suggest
future
optimization
scaffolds.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(8), С. 9768 - 9786
Опубликована: Фев. 13, 2024
This
study
aims
to
overcome
the
drawbacks
associated
with
hydroxyapatite
(HAP)
dense
structures
after
sintering,
which
often
result
in
undesirable
features
such
as
large
grain
size,
reduced
porosity,
high
crystallinity,
and
low
specific
surface
area.
These
characteristics
hinder
osseointegration
limit
clinical
applicability
of
material.
To
address
these
issues,
a
new
method
involving
preparation
hollow
(hHAP)
microspheres
has
been
proposed.
exhibit
distinctive
traits
including
weak
crystallization,
area,
increased
porosity.
The
crystallization
aligns
more
closely
early
mineralization
products
found
human
body
animals.
Moreover,
microspheres'
area
porosity
offer
advantages
for
protein
loading
facilitating
osteoblast
attachment.
innovative
approach
not
only
mitigates
limitations
conventional
HAP
but
also
holds
potential
improving
effectiveness
biomedical
applications,
particularly
enhancing
osseointegration.
Three-dimensional
printed
hHAP/chitosan
(CS)
scaffolds
different
hHAP
concentration
gradients
were
manufactured,
physical
biological
properties
each
group
systematically
evaluated.
In
vitro
vivo
experiments
show
that
hHAP/CS
scaffold
excellent
performance
bone
remodeling.
Furthermore,
in-scaffold
components,
CS
cocultured
marrow
mesenchymal
stem
cells
explore
regulatory
role
process
healing
reveal
cell-level
network
activated
by
hHAP.
Enrichment
analysis
showed
can
promote
regeneration
reconstruction
recruiting
calcium
ions
regulating
inflammatory
reactions.
Materials & Design,
Год журнала:
2022,
Номер
224, С. 111414 - 111414
Опубликована: Ноя. 22, 2022
A
Voronoi
method
is
a
feasible
approach
for
developing
biomimetic
trabecular
scaffolds.
This
study
used
algorithms
based
on
scaling
factors
to
design
scaffolds
(pore
sizes
600–1200
μm).
Scaffolds
were
prepared
from
β-tricalcium
phosphate
slurry
and
3D
printed
using
stereolithography.
Characterization
analysis
revealed
that
the
microporous
structures
correctly
processed.
Computational
fluid
dynamics
indicated
scaffold
permeability
increased
specific
surface
area
decreased
with
increasing
pore
size,
wall
shear
stress
was
highest
in
P800
size-800
In
addition,
MC3T3
cells
dynamically
seeded
more
evenly
distributed
showed
higher
proliferation
differentiation
than
other
three
vitro.
For
vivo
experiment,
implanted
into
rabbit
femur
analyze
degradation
osteogenesis.
Histological
imaging
examinations
new
bone
formation
abundant
inside
of
scaffolds,
which
explained
by
hydrodynamic
response.
optimized
controllable
designing
size
distribution
(458.8–989.1
μm)
an
optimal
choice
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(36), С. 47178 - 47191
Опубликована: Сен. 2, 2024
Guided
bone
regeneration
(GBR)
technology
has
been
demonstrated
to
be
an
effective
method
for
reconstructing
defects.
A
membrane
is
used
cover
the
defect
stop
soft
tissue
from
growing
into
it.
The
biosurface
design
of
barrier
key
technology.
In
this
work,
asymmetric
functional
gradient
Janus
was
designed
address
bidirectional
environment
and
during
reconstruction.
simply
efficiently
prepared
by
multilayer
self-assembly
technique,
it
divided
polycaprolactone
isolation
layer
(PCL
layer,
GBR-A)
nanohydroxyapatite/polycaprolactone/polyethylene
glycol
osteogenic
(HAn/PCL/PEG
GBR-B).
morphology,
composition,
roughness,
hydrophilicity,
biocompatibility,
cell
attachment,
mineralization
ability
double
surfaces
were
systematically
evaluated.
GBR-A
smooth,
dense,
hydrophobic,
which
could
inhibit
adhesion
resist
invasion.
GBR-B
rough,
porous,
hydrophilic,
bioactive,
promoting
adhesion,
proliferation,
matrix
mineralization,
expression
alkaline
phosphatase
RUNX2.
vitro
in
vivo
results
showed
that
bind
tightly
bone,
maintain
long-term
space
stability,
significantly
promote
new
formation.
Moreover,
fix
filling
material
a
better
healing
effect.
This
work
presents
straightforward
viable
methodology
fabrication
GBR
membranes
with
Janus-based
bioactive
surfaces.
may
provide
insights
biomaterial
treatment
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Март 26, 2024
The
increasing
use
of
medical
implants
in
various
areas
medicine,
particularly
orthopedic
surgery,
oncology,
cardiology
and
dentistry,
displayed
the
limitations
long-term
integration
available
biomaterials.
effective
functioning
successful
requires
not
only
technical
excellence
materials
but
also
consideration
dynamics
biomaterial
interaction
with
immune
system
throughout
entire
duration
implant
use.
acute
as
well
decisions
about
efficiency
are
done
by
local
resident
tissue
macrophages
monocyte-derived
that
start
to
be
recruited
during
damage,
when
is
installed,
continuously
healing
phase.
Our
review
summarized
knowledge
currently
used
macrophages-based
vitro
cells
include
murine
human
lines
primary
ex
vivo
differentiated
macrophages.
We
provided
information
most
frequently
examined
biomarkers
for
inflammation,
chronic
foreign
body
response
fibrosis,
indicating
benefits
model
systems.
Particular
attention
given
scavenging
function
controls
dynamic
composition
peri-implant
microenvironment
ensures
timely
clearance
microorganisms,
cytokines,
metabolites,
extracellular
matrix
components,
dying
debris.
outline
perspective
application
3D
systems
modelling
tissue-specific
avoiding
animal
experimentation.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2023,
Номер
11
Опубликована: Фев. 2, 2023
Bones
are
important
for
maintaining
motor
function
and
providing
support
internal
organs.
Bone
diseases
can
impose
a
heavy
burden
on
individuals
society.
Although
bone
has
certain
ability
to
repair
itself,
it
is
often
difficult
itself
alone
when
faced
with
critical-sized
defects,
such
as
severe
trauma,
surgery,
or
tumors.
There
still
reliance
metal
implants
autologous
allogeneic
grafts
defects
that
self-heal.
However,
these
have
problems
circumvent,
may
require
secondary
surgical
removal,
lack
of
graft
donors,
immune
rejection.
The
rapid
advance
in
tissue
engineering
better
comprehension
the
physiological
mechanisms
regeneration
led
new
focus
promoting
endogenous
self-regeneration
through
use
biomaterials
medium.
involves
variety
cells
signaling
factors,
complex
pathways
interaction
not
been
fully
understood,
macrophages
undoubtedly
play
an
essential
role
regeneration.
This
review
summarizes
design
strategies
need
be
considered
regulate
macrophage
Subsequently,
this
provides
overview
therapeutic
intervene
all
stages
by
regulating
macrophages.
Materials Today Bio,
Год журнала:
2023,
Номер
23, С. 100882 - 100882
Опубликована: Дек. 1, 2023
The
clinical
treatments
of
bone
defects
remain
a
challenge.
Hydrogels
containing
marrow
mesenchymal
stem
cells
(BMSCs)
are
extensively
used
to
regeneration
because
excellent
biocompatibility
and
hydrophilicity.
However,
the
insufficient
osteo-induction
capacity
BMSC-loaded
hydrogels
limits
their
applications.
In
this
study,
bio-active
glass
(BG)
BMSCs
were
combined
with
gelatin
methacryloyl
(GelMA)
fabricate
composite
via
photo-crosslinking,
regulation
was
investigated.
vitro
experiments
showed
that
BG/BMSCs@GelMA
hydrogel
had
cytocompatibility
promoted
osteogenic
differentiation
in
BMSCs.
Furthermore,
injected
into
critical-sized
calvarial
defects,
results
further
confirmed
its
angiogenetic
capacity.
addition,
polarization
macrophages
towards
M2
phenotype.
summary,
novel
demonstrated
remarkable
potential
for
application
due
immunomodulatory,
as
well
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(10), С. 5152 - 5152
Опубликована: Май 9, 2024
Cancer
immunotherapy
relies
on
the
insight
that
immune
system
can
be
used
to
defend
against
malignant
cells.
The
aim
of
cancer
is
utilize,
modulate,
activate,
and
train
amplify
antitumor
T-cell
immunity.
In
parallel,
response
damaged
tissue
also
crucial
in
determining
success
or
failure
an
implant.
Due
their
extracellular
matrix
mimetics
tunable
chemical
physical
performance,
hydrogels
are
promising
platforms
for
building
immunomodulatory
microenvironments
realizing
therapy
regeneration.
However,
submicron
nanosized
pore
structures
within
not
favorable
modulating
cell
function,
such
as
invasion,
migration,
immunophenotype.
contrast,
with
a
porous
structure
only
allow
nutrient
transportation
metabolite
discharge
but
offer
more
space
function.
this
review,
design
strategies
influencing
factors
regeneration
first
discussed.
Second,
effects
therapeutic
outcomes
different
highlighted.
Beyond
that,
review
highlights
size
function
potential
signal
transduction.
Finally,
remaining
challenges
perspectives
