Materials Today Bio,
Год журнала:
2025,
Номер
32, С. 101687 - 101687
Опубликована: Март 20, 2025
Repairing
bone
defects
in
inflammatory
conditions
remains
a
significant
clinical
challenge.
An
ideal
scaffold
material
for
such
situations
should
enable
minimally
invasive
implantation
and
integrate
capabilities
immunomodulation,
anti-infection
therapy,
enhanced
regeneration.
In
this
study,
we
developed
injectable
calcitriol@polydopamine@gelatin
methacryloyl
hydrogel
microspheres
(CAL@PDA@GMs)
using
microfluidic
technology.
This
system
facilitates
the
sustained
release
of
calcitriol,
which
features
excellent
biocompatibility
biodegradability,
promotes
osteogenesis,
scavenges
excessive
reactive
oxygen
species
(ROS),
induces
polarization
macrophages
from
M1
to
M2
phenotype,
thereby
mitigating
lipopolysaccharide
(LPS)-induced
inflammation.
These
mechanisms
work
synergistically
create
an
optimal
immune
microenvironment
regeneration
conditions.
RNA
sequencing
(RNA-Seq)
analyses
revealed
that
immunomodulation
is
achieved
by
regulating
macrophage
phenotypes,
inhibiting
nuclear
transcription
factor-kappa
B
(NF-κB)
ROS
signaling
pathways,
reducing
secretion
pro-inflammatory
cytokines.
study
proposes
novel
method
enhance
tissue
remediating
damaged
presents
potential
therapeutic
strategy
large-scale
injuries.
Advanced Functional Materials,
Год журнала:
2023,
Номер
33(43)
Опубликована: Июль 2, 2023
Abstract
Osteochondral
regeneration
remains
a
great
challenge
due
to
the
limited
self‐healing
ability
and
complexity
of
its
hierarchical
structure
composition.
Mg
2+
hypoxia
are
two
effective
modulators
in
boosting
chondrogenesis.
To
this
end,
double‐layered
scaffold
(D)
consisting
hydrogel
layer
on
porous
cryogel
is
fabricated
mimic
osteochondral
tissue.
An
gradient
incorporated
into
with
hypoxia‐mimicking
deferoxamine
(DFO)
embedded
(D‐Mg‐DFO),
which
remarkably
augments
dual‐lineage
both
cartilage
subchondral
bone.
The
higher
supplementation
from
upper
hydrogel,
associated
situation
small
pore
size,
exhibits
promotive
effects
chondrogenic
differentiation.
lower
bottom
cryogel,
interconnected
macroporous
structure,
achieves
multiple
contributions
stem
cell
migration
bone
marrow
cavity,
matrix
mineralization,
osteogenesis.
Furthermore,
rabbits’
trochlea
defects
established
evaluate
regenerative
outcome.
Compared
control
scaffolds
containing
only
or
DFO,
D‐Mg‐DFO
presents
best
effect
under
synergistic
contribution
factors.
Overall,
work
provides
new
design
toward
an
repair
defect.
ABSTRACT
The
repair
and
functional
reconstruction
of
bone
defects
resulting
from
trauma,
surgical
resection,
degenerative
diseases,
congenital
malformations
are
major
clinical
challenges.
Bone
tissue
engineering
has
significant
advantages
in
the
treatment
severe
defects.
Vascularized
scaffolds
gradually
attracting
attention
development
because
their
excellent
biomimetic
properties
efficient
efficiency.
Three‐dimensional
(3D)
printing
technology,
which
can
be
used
to
fabricate
structures
at
different
scales
using
a
wide
range
materials,
been
production
vascularized
scaffolds.
This
review
discusses
research
progress
3D
for
Angiogenesis‐osteogenesis
coupling
regeneration
process
is
first
introduced,
followed
by
summary
technologies,
inks,
bioactive
factors
Notably,
this
focuses
on
structural
design
strategies
Finally,
application
medicine,
as
well
challenges
outlooks
future
development,
described.
Biomaterials Science,
Год журнала:
2024,
Номер
12(5), С. 1079 - 1114
Опубликована: Янв. 1, 2024
Hydrogels,
formed
from
crosslinked
hydrophilic
macromolecules,
provide
a
three-dimensional
microenvironment
that
mimics
the
extracellular
matrix.
They
served
as
scaffold
materials
in
regenerative
medicine
with
an
ever-growing
demand.
However,
hydrogels
composed
of
only
organic
components
may
not
fully
meet
performance
and
functionalization
requirements
for
various
tissue
defects.
Composite
hydrogels,
containing
inorganic
components,
have
attracted
tremendous
attention
due
to
their
unique
compositions
properties.
Rigid
particles,
rods,
fibers,
Abstract
The
effective
management
of
osteomyelitis
remains
extremely
challenging
due
to
the
difficulty
associated
with
treating
bone
defects,
high
probability
recurrence,
requirement
secondary
surgery
or
multiple
surgeries,
and
in
eradicating
infections
caused
by
methicillin‐resistant
Staphylococcus
aureus
(MRSA).
Hence,
smart
biodegradable
biomaterials
that
provide
precise
local
anti‐infection
effects
can
promote
repair
defects
are
actively
being
developed.
Here,
a
novel
nano‐micro
composite
is
fabricated
combining
calcium
phosphate
(CaP)
nanosheets
drug‐loaded
GelMA
microspheres
via
microfluidic
technology.
covalently
linked
vancomycin
(Van)
through
an
oligonucleotide
(oligo)
linker
using
EDC/NHS
carboxyl
activator.
Accordingly,
called
“CaP@MS‐Oligo‐Van”
synthesized.
porous
CaP@MS‐Oligo‐Van
composites
target
capture
bacteria.
They
also
release
Van
response
presence
bacterial
micrococcal
nuclease
Ca
2+
,
exerting
additional
antibacterial
inhibiting
inflammatory
response.
Finally,
released
CaP
tissue
repair.
Overall,
findings
show
rapid,
targeted
drug
system
based
on
effectively
infections.
this
agent
holds
potential
clinical
treatment
MRSA.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(34)
Опубликована: Март 10, 2024
Abstract
Native
bone
inherently
requires
a
balanced
ionic
microenvironment
to
maintain
homeostasis.
Hence,
scaffolds
designed
for
the
sustained
release
of
therapeutic
ions
into
defects
hold
great
promise
regeneration.
Magnesium
(Mg)
and
silicon
(Si)
are
essential
elements,
which
play
crucial
roles
in
process
regeneration,
impacting
immunomodulation,
angiogenesis,
osteogenesis.
Herein,
porous
cryogel‐type
organic–inorganic
composite
microspheres
developed
as
injectable
microscaffolds
(denoted
GMN).
GMN
enables
Mg/Si
at
an
optimized
ratio,
achieving
most
significant
synergistic
effect
on
vascularized
Various
conditioned
media
obtained
explore
angiogenesis‐osteogenesis
coupling,
well
crosstalk
between
marrow
mesenchymal
stromal
cells
(BMSCs)
macrophages.
Meanwhile,
autocrine
paracrine
effects
simultaneously
modulating
functions
determining
cell
fates
under
guidance
biofactors
secreted
by
cells.
Overall,
ion‐engineering
create
conducive
efficiently
augment
regeneration
tissue
vivo,
offering
versatile
platform
engineering.
Advanced Materials,
Год журнала:
2024,
Номер
36(33)
Опубликована: Июнь 6, 2024
The
growing
aging
population,
with
its
associated
chronic
diseases,
underscores
the
urgency
for
effective
tissue
regeneration
strategies.
Biomaterials
play
a
pivotal
role
in
realm
of
reconstruction
and
regeneration,
distinct
shift
toward
minimally
invasive
(MI)
treatments.
This
transition,
fueled
by
engineered
biomaterials,
steers
away
from
surgical
procedures
to
embrace
approaches
offering
reduced
trauma,
accelerated
recovery,
cost-effectiveness.
In
MI
repair
cargo
delivery,
various
techniques
are
explored.
While
situ
polymerization
is
prominent,
it
not
without
challenges.
narrative
review
explores
diverse
fabrication
methods,
biofunctionalization
injectable
pre-formed
scaffolds,
focusing
on
their
unique
advantages.
exhibiting
compressibility,
controlled
injection,
maintained
mechanical
integrity,
emerge
as
promising
alternative
solutions
conclusion
this
emphasizes
importance
interdisciplinary
design
facilitated
synergizing
fields
materials
science,
advanced
3D
biomanufacturing,
mechanobiological
studies,
innovative
regeneration.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 18, 2025
Abstract
Contemporary
clinical
interventions
for
cartilage
injuries
focus
on
symptom
management
through
pharmaceuticals
and
surgical
procedures.
Recent
research
has
aimed
at
developing
innovative
scaffolds
with
biochemical
elements,
yet
challenges
like
inadequate
targeted
delivery
reduced
load‐bearing
capacity
hinder
their
adoption.
Inspired
by
the
spatial
gradients
of
biophysical
cues
in
native
osteochondral
tissues,
a
silk‐based
hydrogel
that
facilitates
spontaneous
dual‐gradient
formation,
including
mechanical
growth
factor
gradients,
tissue
regeneration,
is
presented.
Driven
an
electrical
field,
transitions
from
stiff
to
soft
along
anode‐to‐cathode
direction,
mimicking
anisotropic
structure
natural
tissues.
Simultaneously,
incorporated
factors
encapsulated
charged
monomers
migrate
cathode
region,
creating
another
parallel
gradient
enables
sustained
release.
This
design
maintains
bioactivity
enhances
programmable
concentration
defect
environment.
In
rabbit
model
full‐thickness
defects,
demonstrates
significant
potential
promoting
offering
promising
tool
translation.
