Target-Triggered Ultrafast Chondroitin Gelation Enabled Power-Free and Point-of-Care Bioassays DOI

Chunjiao Qi,

Jintao Chen, Yilun Shang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(44), С. 17781 - 17788

Опубликована: Окт. 22, 2024

Point-of-care (POC) tests increasingly highlight the importance of portable, cost-effective, and visually quantitative detection biomarkers. Herein, we developed a power-free visual signal-readout POC sensor based on target-triggered ultrafast gelation process. In process, target triggered cascade reaction catalyzed by oxidase ferrous glycinate to produce carbon radicals that immediately initiated rapid polymerization cross-linking acryloylated chondroitin sulfate dimethylacrylamide. This highly efficient enzymatic process contributed generation hydrogel within 1 min at 25 °C. The increase in viscosity aqueous solution resulted from formation was then measured according distance migration tick-labeled pH test strip, which thus realized quantification target. By utilizing glucose as an model during this successfully employed without need for any auxiliary instruments. Benefiting specificity stability reaction, exhibited excellent performance clinical blood samples. Moreover, further extended uric acid enabled accurate assay urine samples, indicated versatility practicability test.

Язык: Английский

Tailoring DNA Surface Interactions on Single-Layer Graphene: Comparative Analysis of Pyrene, Acridine, and Fluorenyl Methyl Linkers DOI
Telma Domingues, Chun‐Da Liao, Marta Prado

и другие.

Langmuir, Год журнала: 2024, Номер unknown

Опубликована: Дек. 23, 2024

This study investigates the effect of different linkers and solvents on immobilization DNA probes graphene surfaces, which are crucial for developing high-performance biosensors. Quartz crystal microbalance with dissipation (QCM-D) measurements were used to characterize in situ real-time ssDNA hybridization efficiency model surfaces. The kinetics thermodynamics systematically investigated all pairings between three bifunctional linkers─1-pyrenebutyric acid succinimidyl ester (PBSE), Fluorenylmethylsuccinimidyl carbonate (FSC), Acridine Orange (AO) ester─and organic (DMF, DMSO, 10% DMF/ethanol). linker's spatial orientation effective surface modification probe attachment also evaluated based footprints coverage. Graphene surfaces functionalized PBSE DMF achieved highest density (up 1.31 × 10

Язык: Английский

Процитировано

1
Target-Triggered Ultrafast Chondroitin Gelation Enabled Power-Free and Point-of-Care Bioassays DOI

Chunjiao Qi,

Jintao Chen, Yilun Shang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(44), С. 17781 - 17788

Опубликована: Окт. 22, 2024

Point-of-care (POC) tests increasingly highlight the importance of portable, cost-effective, and visually quantitative detection biomarkers. Herein, we developed a power-free visual signal-readout POC sensor based on target-triggered ultrafast gelation process. In process, target triggered cascade reaction catalyzed by oxidase ferrous glycinate to produce carbon radicals that immediately initiated rapid polymerization cross-linking acryloylated chondroitin sulfate dimethylacrylamide. This highly efficient enzymatic process contributed generation hydrogel within 1 min at 25 °C. The increase in viscosity aqueous solution resulted from formation was then measured according distance migration tick-labeled pH test strip, which thus realized quantification target. By utilizing glucose as an model during this successfully employed without need for any auxiliary instruments. Benefiting specificity stability reaction, exhibited excellent performance clinical blood samples. Moreover, further extended uric acid enabled accurate assay urine samples, indicated versatility practicability test.

Язык: Английский

Процитировано

0