Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(44), P. 17781 - 17788
Published: Oct. 22, 2024
Point-of-care
(POC)
tests
increasingly
highlight
the
importance
of
portable,
cost-effective,
and
visually
quantitative
detection
biomarkers.
Herein,
we
developed
a
power-free
visual
signal-readout
POC
sensor
based
on
target-triggered
ultrafast
gelation
process.
In
process,
target
triggered
cascade
reaction
catalyzed
by
oxidase
ferrous
glycinate
to
produce
carbon
radicals
that
immediately
initiated
rapid
polymerization
cross-linking
acryloylated
chondroitin
sulfate
dimethylacrylamide.
This
highly
efficient
enzymatic
process
contributed
generation
hydrogel
within
1
min
at
25
°C.
The
increase
in
viscosity
aqueous
solution
resulted
from
formation
was
then
measured
according
distance
migration
tick-labeled
pH
test
strip,
which
thus
realized
quantification
target.
By
utilizing
glucose
as
an
model
during
this
successfully
employed
without
need
for
any
auxiliary
instruments.
Benefiting
specificity
stability
reaction,
exhibited
excellent
performance
clinical
blood
samples.
Moreover,
further
extended
uric
acid
enabled
accurate
assay
urine
samples,
indicated
versatility
practicability
test.
Langmuir,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 23, 2024
This
study
investigates
the
effect
of
different
linkers
and
solvents
on
immobilization
DNA
probes
graphene
surfaces,
which
are
crucial
for
developing
high-performance
biosensors.
Quartz
crystal
microbalance
with
dissipation
(QCM-D)
measurements
were
used
to
characterize
in
situ
real-time
ssDNA
hybridization
efficiency
model
surfaces.
The
kinetics
thermodynamics
systematically
investigated
all
pairings
between
three
bifunctional
linkers─1-pyrenebutyric
acid
succinimidyl
ester
(PBSE),
Fluorenylmethylsuccinimidyl
carbonate
(FSC),
Acridine
Orange
(AO)
ester─and
organic
(DMF,
DMSO,
10%
DMF/ethanol).
linker's
spatial
orientation
effective
surface
modification
probe
attachment
also
evaluated
based
footprints
coverage.
Graphene
surfaces
functionalized
PBSE
DMF
achieved
highest
density
(up
1.31
×
10
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(44), P. 17781 - 17788
Published: Oct. 22, 2024
Point-of-care
(POC)
tests
increasingly
highlight
the
importance
of
portable,
cost-effective,
and
visually
quantitative
detection
biomarkers.
Herein,
we
developed
a
power-free
visual
signal-readout
POC
sensor
based
on
target-triggered
ultrafast
gelation
process.
In
process,
target
triggered
cascade
reaction
catalyzed
by
oxidase
ferrous
glycinate
to
produce
carbon
radicals
that
immediately
initiated
rapid
polymerization
cross-linking
acryloylated
chondroitin
sulfate
dimethylacrylamide.
This
highly
efficient
enzymatic
process
contributed
generation
hydrogel
within
1
min
at
25
°C.
The
increase
in
viscosity
aqueous
solution
resulted
from
formation
was
then
measured
according
distance
migration
tick-labeled
pH
test
strip,
which
thus
realized
quantification
target.
By
utilizing
glucose
as
an
model
during
this
successfully
employed
without
need
for
any
auxiliary
instruments.
Benefiting
specificity
stability
reaction,
exhibited
excellent
performance
clinical
blood
samples.
Moreover,
further
extended
uric
acid
enabled
accurate
assay
urine
samples,
indicated
versatility
practicability
test.
