Unveiling Invisible Extracellular Vesicles: Cutting‐Edge Technologies for Their in Vivo Visualization DOI Creative Commons
Prakash Gangadaran, Fatima Khan, Ramya Lakshmi Rajendran

и другие.

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Год журнала: 2024, Номер 16(5)

Опубликована: Сен. 1, 2024

ABSTRACT Extracellular vesicles (EVs), nanosized lipid bilayer released by nearly all types of cells, play pivotal roles as intercellular signaling mediators with diverse biological activities. Their adaptability has attracted interest in exploring their role disease biomarker theranostics. However, the vivo biodistribution and pharmacokinetic profiles EVs, particularly following administration into living subjects, remain unclear. Thus, imaging is vital to enhance our understanding homing retention patterns, blood tissue half‐life, excretion pathways exogenous thereby advancing real‐time monitoring within systems therapeutic applications. This review examines state‐of‐the‐art methods including EV labeling various agents, optical imaging, magnetic resonance nuclear imaging. The strengths weaknesses each technique are comprehensively explored, emphasizing clinical translation. Despite potential EVs cancer theranostics, achieving a thorough behavior challenging. highlights urgency addressing current questions biology applications EVs. It underscores need for continued research unravel complexities surrounding implications. By identifying these challenges, this contributes ongoing efforts optimize techniques use. Ultimately, bridging gap between advancements will facilitate integration EV‐based marking crucial step toward harnessing full medical practice.

Язык: Английский

Surface Engineering of Magnetic Iron Oxide Nanoparticles for Breast Cancer Diagnostics and Drug Delivery DOI Creative Commons

Mengjie Xie,

Fansu Meng,

Panpan Wang

и другие.

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 8437 - 8461

Опубликована: Авг. 1, 2024

Data published in 2020 by the International Agency for Research on Cancer (IARC) of World Health Organization show that breast cancer (BC) has become most common globally, affecting more than 2 million women each year. The complex tumor microenvironment, drug resistance, metastasis, and poor prognosis constitute primary challenges current diagnosis treatment BC. Magnetic iron oxide nanoparticles (MIONPs) have emerged as a promising nanoplatform diagnostic imaging well therapeutic drug-targeted delivery due to their unique physicochemical properties. extensive surface engineering given rise multifunctionalized MIONPs. In this review, latest advancements modification strategies MIONPs over past five years are summarized categorized constrast agents platforms. Additionally, remaining future prospects MIONPs-based targeted discussed.

Язык: Английский

Процитировано

5

Recent advance on extracellular vesicle labeling: From strategy to probe DOI
Sijung Hu,

Xinwen Chang,

Qiaojiao Ding

и другие.

Coordination Chemistry Reviews, Год журнала: 2025, Номер 534, С. 216601 - 216601

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0

Magnetically Labeled iPSC-Derived Extracellular Vesicles Enable MRI/MPI-Guided Regenerative Therapy for Myocardial Infarction DOI Creative Commons

Wenshen Wang,

Zheng Han, Safiya Aafreen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Abstract Stem cell-derived extracellular vesicles (EVs) offer a promising cell-free approach for cardiovascular regenerative medicine. In this study, we developed magnetically labeled induced pluripotent stem EVs (magneto-iPSC-EVs) encapsulated with superparamagnetic iron oxide (SPIO) nanoparticles image-guided treatment of myocardial infarction, in which that can be detected by both magnetic resonance imaging (MRI) and particle (MPI). iPSC-EVs were isolated, characterized per MISEV2023 guidelines, loaded SuperSPIO20 using optimized electroporation conditions (300 V, 2 × 10 ms pulses), achieving high loading efficiency 1.77 ng Fe/10 6 EVs. vitro results show magneto-iPSC-EVs sensitively MPI MRI, detectability approximately 7 mouse ischemia-reperfusion model, intramyocardially injected (2 9 ) imaged non-invasively vivo days ex the presence confirmed Prussian blue staining. Therapeutically, native significantly improved cardiac function, 37.3% increase left ventricular ejection fraction 61.0% reduction scar size. This study highlights potential as medicine, offering non-invasive capabilities therapeutic benefits repair.

Язык: Английский

Процитировано

0

Development of Iron Oxide Nanochains as a Sensitive Magnetic Particle Imaging Tracer for Cancer Detection DOI Creative Commons
P. P. Praveen Kumar,

Md Nafiujjaman,

Ashley V. Makela

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

The advancement of imaging technologies plays a crucial role in improving the diagnosis and monitoring diseases, including cancer. This study introduces new design iron oxide-based nanoparticles specifically developed for magnetic particle (MPI), aimed at tracking diagnosing breast cancer more effectively. By precisely controlling size, shape, properties these nanoparticles, we enhance responsiveness MPI, resulting an increased signal. In our research, established novel synthetic route fabricating oxide nanochains (FeONCs) characterized by their uniform shape which contribute to high suitable MPI applications. Initial results indicate FeONCs exhibit superior compared conventional spherical superparamagnetic nanocubes, reported nanoworm-type structures. Magnetic relaxometry studies revealed that provide higher sensitivity than commonly used VivoTrax Synomag D50 D70 MPI. Further, size significantly influence cellular uptake. vivo experiments using orthotopic mouse models allow us assess biocompatibility characteristics confirming efficacy. Furthermore, conjugating with RGD peptide, ability target cancer, establishing them as promising tracers applications sensitivity. Thus, findings highlight improve quality, facilitating early detection accurate paves way innovative diagnostic strategies personalized treatment options. Future research will focus on fine-tuning surface chemistry further targeting efficiency optimization practice clinical applications, particularly MPI-based hyperthermia therapy.

Язык: Английский

Процитировано

0

Unveiling Invisible Extracellular Vesicles: Cutting‐Edge Technologies for Their in Vivo Visualization DOI Creative Commons
Prakash Gangadaran, Fatima Khan, Ramya Lakshmi Rajendran

и другие.

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Год журнала: 2024, Номер 16(5)

Опубликована: Сен. 1, 2024

ABSTRACT Extracellular vesicles (EVs), nanosized lipid bilayer released by nearly all types of cells, play pivotal roles as intercellular signaling mediators with diverse biological activities. Their adaptability has attracted interest in exploring their role disease biomarker theranostics. However, the vivo biodistribution and pharmacokinetic profiles EVs, particularly following administration into living subjects, remain unclear. Thus, imaging is vital to enhance our understanding homing retention patterns, blood tissue half‐life, excretion pathways exogenous thereby advancing real‐time monitoring within systems therapeutic applications. This review examines state‐of‐the‐art methods including EV labeling various agents, optical imaging, magnetic resonance nuclear imaging. The strengths weaknesses each technique are comprehensively explored, emphasizing clinical translation. Despite potential EVs cancer theranostics, achieving a thorough behavior challenging. highlights urgency addressing current questions biology applications EVs. It underscores need for continued research unravel complexities surrounding implications. By identifying these challenges, this contributes ongoing efforts optimize techniques use. Ultimately, bridging gap between advancements will facilitate integration EV‐based marking crucial step toward harnessing full medical practice.

Язык: Английский

Процитировано

3