Aromatic side-chain crosslinking in RiPP biosynthesis

Nature Chemical Biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Язык: Английский

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Radical SAM Enzyme WprB Catalyzes Uniform Cross-Link Topology between Trp-C5 and Arg-Cγ on the Precursor Peptide

ACS Chemical Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Cross-link containing products from ribosomally synthesized and post-translationally modified peptides (RiPPs) are generated by radical SAM enzymes (rSAM). Here, we bioinformatically expanded rSAM based on the known families StrB, NxxcB, WgkB, RrrB, TqqB GggB. Through in vivo functional studies E. coli, newly identified enzyme WprB Xenorhabdus sp. psl was found to catalyze formation of a cross-link between Trp-C5 Arg-Cγ at three WPR motifs precursor peptide WprA. This represents first report this type enzymes.

Язык: Английский

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Fused radical SAM and αKG-HExxH domain proteins contain a distinct structural fold and catalyse cyclophane formation and β-hydroxylation

Nature Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Сен. 18, 2024

Язык: Английский

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Radical SAM cyclophane synthase catalyzes uniform cyclophane formation at multiple Trp-motifs

Tetrahedron Chem, Год журнала: 2025, Номер unknown, С. 100128 - 100128

Опубликована: Март 1, 2025

Язык: Английский

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Genome mining and in vivo characterization of triceptides

Methods in enzymology on CD-ROM/Methods in enzymology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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