Radical SAM cyclophane synthase catalyzes uniform cyclophane formation at multiple Trp-motifs DOI Creative Commons
Abujunaid Khan, Jabal Rahmat Haedar, Anitra Zīle

et al.

Tetrahedron Chem, Journal Year: 2025, Volume and Issue: unknown, P. 100128 - 100128

Published: March 1, 2025

Language: Английский

Aromatic side-chain crosslinking in RiPP biosynthesis DOI
Sanath K. Kandy, Michael A. Pasquale, Jonathan R. Chekan

et al.

Nature Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Language: Английский

Citations

1
Radical SAM Enzyme WprB Catalyzes Uniform Cross-Link Topology between Trp-C5 and Arg-Cγ on the Precursor Peptide DOI Creative Commons
Abujunaid Khan, Jabal Rahmat Haedar,

Vic Kiselov

et al.

ACS Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Cross-link containing products from ribosomally synthesized and post-translationally modified peptides (RiPPs) are generated by radical SAM enzymes (rSAM). Here, we bioinformatically expanded rSAM based on the known families StrB, NxxcB, WgkB, RrrB, TqqB GggB. Through in vivo functional studies E. coli, newly identified enzyme WprB Xenorhabdus sp. psl was found to catalyze formation of a cross-link between Trp-C5 Arg-Cγ at three WPR motifs precursor peptide WprA. This represents first report this type enzymes.

Language: Английский

Citations

1
Fused radical SAM and αKG-HExxH domain proteins contain a distinct structural fold and catalyse cyclophane formation and β-hydroxylation DOI
Yohei Morishita, Suze Ma, E. De la Mora

et al.

Nature Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Language: Английский

Citations

6
Radical SAM cyclophane synthase catalyzes uniform cyclophane formation at multiple Trp-motifs DOI Creative Commons
Abujunaid Khan, Jabal Rahmat Haedar, Anitra Zīle

et al.

Tetrahedron Chem, Journal Year: 2025, Volume and Issue: unknown, P. 100128 - 100128

Published: March 1, 2025

Language: Английский

Citations

0