Cardiolipin remodeling maintains the inner mitochondrial membrane in cells with saturated lipidomes

Journal of Lipid Research, Год журнала: 2024, Номер 65(8), С. 100601 - 100601

Опубликована: Июль 20, 2024

Язык: Английский

---

Look Beyond Plasma Membrane Biophysics: Revealing Considerable Variability of the Dipole Potential Between Plasma and Organelle Membranes of Living Cells

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 889 - 889

Опубликована: Янв. 22, 2025

Due to the lack of measurement techniques suitable for examining compartments intact, living cells, membrane biophysics is almost exclusively investigated in plasma despite fact that its alterations intracellular organelles may also contribute disease pathogenesis. Here, we employ a novel, easy-to-use, confocal microscopy-based approach utilizing F66, an environment-sensitive fluorophore combination with fluorescent organelle markers and quantitative image analysis determine magnitude molecular order-related dipole potential various tumor neural cell lines. Our comparative demonstrates considerable variations be large enough modulate protein functions, inward decreasing gradient on route secretory/endocytic pathway (plasma >> lysosome > Golgi endoplasmic reticulum), whereas mitochondrial membranes are characterized by slightly larger than lysosomes. sensitive quantify biophysical properties selectively their and, therefore, identify affected therapeutic targets diseases associated lipid composition thus such as tumors, metabolic, neurodegenerative, or lysosomal storage disorders.

Язык: Английский

---

HBimmCue: A Versatile Fluorescent Probe for Multi‐Scale Imaging of Lipid Polarity and Membrane Order in Inner Mitochondrial Membrane

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 9, 2025

Abstract Mitochondrial membrane environmental dynamics are crucial for understanding function, yet high‐resolution observation remains challenging. Here, HBimmCue is introduced as a fluorescent probe localized to inner mitochondrial (IMM) that reports lipid polarity and order changes, which correlate with cellular respiration levels. Using fluorescence lifetime imaging microscopy (FLIM), IMM heterogeneity uncovered across scales, from nanoscale structures within individual mitochondria mouse pre‐implantation embryos. At the sub‐organelle level, stimulated emission depletion (STED)‐FLIM highlights variations IMM. sub‐cellular reduced observed in damaged marked lysosomal degradation distinct distributions identified neurons disease models. Additionally, metabolic dysfunction associated oocytes aging reprogramming zygote blastocyst detected. Together, work demonstrates broad applicability of HBimmCue, offering new paradigm investigating level at multiple scales.

Язык: Английский

---

Editorial: Novel insights into the modulation of protein function by lipids and membrane organization

Frontiers in Cell and Developmental Biology, Год журнала: 2025, Номер 13

Опубликована: Апрель 24, 2025

Язык: Английский

---

Setting the curve: the biophysical properties of lipids in mitochondrial form and function

Journal of Lipid Research, Год журнала: 2024, Номер unknown, С. 100643 - 100643

Опубликована: Сен. 1, 2024

Язык: Английский

---