lncRNA Biomarkers of Glioblastoma Multiforme

Biomedicines, Год журнала: 2024, Номер 12(5), С. 932 - 932

Опубликована: Апрель 23, 2024

Long noncoding RNAs (lncRNAs) are RNA molecules of 200 nucleotides or more in length that not translated into proteins. Their expression is tissue-specific, with the vast majority involved regulation cellular processes and functions. Many human diseases, including cancer, have been shown to be associated deregulated lncRNAs, rendering them potential therapeutic targets biomarkers for differential diagnosis. The lncRNAs nervous system varies different cell types, implicated mechanisms neurons glia, effects on development functioning brain. Reports also a link between changes lncRNA etiopathogenesis brain neoplasia, glioblastoma multiforme (GBM). GBM an aggressive variant cancer unfavourable prognosis median survival 14-16 months. It considered brain-specific disease highly invasive malignant cells spreading throughout neural tissue, impeding complete resection, leading post-surgery recurrences, which prime cause mortality. early diagnosis could improve treatment extend survival, profiling biological fluids promising detection neoplastic at their initial stages effective interventions. This review presents systematic overview GBM-associated deregulation focus fingerprints patients' blood.

Язык: Английский

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Competing Endogenous RNA Networks as Biomarkers in Neurodegenerative Diseases

International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(24), С. 9582 - 9582

Опубликована: Дек. 16, 2020

Protein aggregation is classically considered the main cause of neuronal death in neurodegenerative diseases (NDDs). However, increasing evidence suggests that alteration RNA metabolism a key factor etiopathogenesis these complex disorders. Non-coding RNAs are major contributor to human transcriptome and particularly abundant central nervous system, where they have been proposed be involved onset development NDDs. Interestingly, some ncRNAs (such as lncRNAs, circRNAs pseudogenes) share common functionality their ability regulate gene expression by modulating miRNAs phenomenon known competing endogenous mechanism. Moreover, found body fluids presence concentration could serve potential non-invasive biomarkers In this review, we summarize ceRNA networks described Alzheimer’s disease, Parkinson’s multiple sclerosis, amyotrophic lateral sclerosis spinocerebellar ataxia type 7, discuss Although numerous studies carried out, further research needed validate interactions between alterations editing provide specific ceRNET profiles for disorders, paving way better understanding diseases.

Язык: Английский

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Non-Coding RNAs in Neurological and Neuropsychiatric Disorders: Unraveling the Hidden Players in Disease Pathogenesis

Cells, Год журнала: 2024, Номер 13(12), С. 1063 - 1063

Опубликована: Июнь 19, 2024

Neurological and neuropsychiatric disorders pose substantial challenges to public health, necessitating a comprehensive understanding of the molecular mechanisms underlying their pathogenesis. In recent years, focus has shifted toward intricate world non-coding RNAs (ncRNAs), class RNA molecules that do not encode proteins but play pivotal roles in gene regulation cellular processes. This review explores emerging significance ncRNAs context neurological disorders, shedding light on diverse functions regulatory mechanisms. The dysregulation various ncRNAs, including microRNAs (miRNAs), long (lncRNAs), circular (circRNAs), been implicated pathophysiology conditions such as Alzheimer’s disease, Parkinson’s schizophrenia, mood disorders. delves into specific these modulating key processes, synaptic plasticity, neuroinflammation, apoptosis, providing nuanced impact disease progression. Furthermore, it discusses potential diagnostic therapeutic implications targeting identification ncRNA signatures holds promise for development novel biomarkers early detection, while manipulation expression offers innovative avenues. Challenges future directions field are also considered, highlighting need continued research unravel complexities ncRNA-mediated networks aims provide overview current state knowledge stimulate further exploration fascinating realm brain’s landscape.

Язык: Английский

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MAGI2-AS3 and miR-374b-5p as Putative Regulators of Multiple Sclerosis via Modulating the PTEN/AKT/IRF-3/IFN-β Axis: New Clinical Insights

ACS Chemical Neuroscience, Год журнала: 2023, Номер 14(6), С. 1107 - 1118

Опубликована: Март 6, 2023

Multiple sclerosis (MS) is a chronic disease and one of the leading causes disability in young adults. The current study aims to investigate pathogenesis MS via studying regulatory role novel lncRNA MAGI2-AS3 miR-374b-5p their downstream targets PTEN/AKT/IRF-3/IFN-β relationship this pathway with severity. Moreover, it assess MAGI2-AS3/miR-374b-5p as diagnostic and/or prognostic biomarkers for MS. Overall, 150 contributors were recruited: 100 patients 50 healthy volunteers. Gene expression MAGI2-AS3, miR-374b-5p, PTEN, AKT, IRF-3 assessed using RT-qPCR, IFN-β was measured by ELISA. Compared control group, serum PTEN downregulated patients, whereas PI3K, IRF-3, upregulated patients. Furthermore, downregulated, while an expanded status scale (EDSS) ≥3.5, compared EDSS <3.5. Receiver-operating-characteristic curve analysis revealed that can be used diagnosis Remarkably, multivariate logistic AKT act independent variables directly correlated inversely EDSS. Regarding positively In conclusion, showed first time crosstalk between could affect AKT/IRF3/IFN-β axis Interestingly, genetic noninvasive

Язык: Английский

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Brain alarm by self-extracellular nucleic acids: from neuroinflammation to neurodegeneration

Journal of Biomedical Science, Год журнала: 2023, Номер 30(1)

Опубликована: Авг. 7, 2023

Abstract Neurological disorders such as stroke, multiple sclerosis, well the neurodegenerative diseases Parkinson's or Alzheimer's disease are accompanied even powered by danger associated molecular patterns (DAMPs), defined endogenous molecules released from stressed damaged tissue. Besides protein-related DAMPs “alarmins”, numerous nucleic acid exist in body fluids, cell-free nuclear and mitochondrial DNA different species of extracellular RNA, collectively termed self-extracellular acids (SENAs). Among these, microRNA, long non-coding RNAs, circular RNAs ribosomal RNA constitute majority RNA-based DAMPs. Upon tissue injury, necrosis apoptosis, SENAs neuronal, immune other cells predominantly association with vesicles may be translocated to target where they can induce intracellular regulatory pathways gene transcription translation. The SENA-induced signaling reactions brain appear related neuroinflammatory processes, often causally onset progression respective disease. In this review, impact diverse types on will discussed. Based accumulating knowledge field, several specific antagonistic approaches presented that could serve therapeutic interventions lower pathological outcome indicated disorders.

Язык: Английский

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Long Noncoding RNAs MALAT1 and ANRIL Gene Variants and the Risk of Cerebral Ischemic Stroke: An Association Study

ACS Chemical Neuroscience, Год журнала: 2021, Номер 12(8), С. 1351 - 1362

Опубликована: Апрель 5, 2021

Cerebral ischemic stroke (CIS) is one of the primary causes death worldwide and a major cause long-term disability. Long noncoding RNAs (lncRNAs) have emerged as crucial mediators in pathology CIS; however, their potential importance yet to be discovered. Herein, we examined association four single-nucleotide polymorphisms (SNPs) with risk CIS, correlation lncRNAs, MALAT1 ANRIL, expression, serum ANRIL biomarkers for CIS. A total 100 CIS patients healthy controls were recruited study. Genotyping expression analysis SNPs carried out by qPCR. The present results showed that was downregulated, while overexpressed patients, relative controls. downregulation discriminated from receiver-operating-characteristic analysis. Moreover, denoted good diagnostic accuracy. rs619586 AA rs3200401 CT, TT associated increased risk, whereas rs10965215 GG found protective. studied rs10738605 polymorphism not susceptibility. Notably, G variant demonstrated higher level. Multivariate logistic regression revealed well CT + independent predictors Additionally, negative between level National Institutes Health Stroke Scale score. In conclusion, are novel prospective noninvasive predisposition.

Язык: Английский

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Non-coding RNAs in immunoregulation and autoimmunity: Technological advances and critical limitations

Journal of Autoimmunity, Год журнала: 2022, Номер 134, С. 102982 - 102982

Опубликована: Дек. 31, 2022

Immune cell function is critically dependent on precise control over transcriptional output from the genome. In this respect, integration of environmental signals that regulate gene expression, specifically by transcription factors, enhancer DNA elements, genome topography and non-coding RNAs (ncRNAs), are key components. The first three have been extensively investigated. Even though represent vast majority cellular RNA species, class remains historically understudied. This partly because a lag in technological bioinformatic innovations capable identifying accurately measuring their expression. Nevertheless, recent progress domain has enabled profusion publications novel sub-types ncRNAs studies directly addressing human health disease. Many ncRNAs, including circular RNAs, now demonstrated to play functions regulation immune cells show associations with immune-mediated diseases. Some may as biomarkers disease, aiding diagnostics estimating response treatment, while others direct role pathogenesis Importantly, some relatively stable amenable therapeutic targeting, for example through therapy. Here, we provide an overview review advances enable study hold substantial promise future. We context-specific examples examining four prototypical autoimmune diseases, rheumatoid arthritis, psoriasis, inflammatory bowel disease multiple sclerosis. anticipate utility mechanistic roles these autoimmunity will be further elucidated near

Язык: Английский

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Virtual reality-based neuroproprioceptive physiotherapy in multiple sclerosis: a protocol for a double-arm randomised assessor-blinded controlled trial on upper extremity function, postural function and quality of life, with molecular and functional MRI assessment

BMJ Open, Год журнала: 2025, Номер 15(1), С. e088046 - e088046

Опубликована: Янв. 1, 2025

Introduction Upper limb (UL) impairment is common in people with multiple sclerosis (pwMS), and functional recovery of the UL a key rehabilitation goal. Technology-based approaches, like virtual reality (VR), are increasingly promising. While most VR environments task-oriented, our clinical approach integrates neuroproprioceptive ‘facilitation inhibition’ (NFI) principles. To advance this, we developed immersive software based on NFI principles targeting function sit-to-stand ability. This study aims to evaluate effectiveness this therapy compared conventional NFI-based physical pwMS. Our uniquely applies advanced imaging techniques, along biological molecular assessments, explore adaptive processes induced by rehabilitation. Methods analysis double-arm, randomised, assessor-blinded, controlled trial runs over 2 months (1 hour, times per week). PwMS mild severe disability will receive either or real-world therapy. Primary outcomes include nine-hole peg test, box block handgrip strength, tremor five test. Secondary measures Multiple Sclerosis Impact Scale, 5-level EQ-5D questionnaire kinematic analysis. Adaptive be monitored using techniques (functional MRI tractography), genetic methods (long non-coding RNAs) immune system markers (leukocytes, dendritic cells). The International Classification Functioning, Disability Health brief set for MS map bio-psycho-social context participants. Ethics dissemination project its amendments were approved Committee Institute Clinical Experimental Medicine Thomayer Hospital (1983/21+4772/21 (G-21–02) Kralovske Vinohrady University (EK-VP/38/0/2021) Prague, Czechia (with single enrolment). findings disseminated through scientific publications, conferences, professional networks, public engagement, educational materials stakeholder briefings ensure broad impact across clinical, academic domains. Trial registration number clinicaltrials.gov ( NCT04807738 ).

Язык: Английский

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Advances in RNA therapy for the treatment of autoimmune diseases

Autoimmunity Reviews, Год журнала: 2025, Номер unknown, С. 103753 - 103753

Опубликована: Янв. 1, 2025

Язык: Английский

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Association of Epistatic Effects of lncRNA GAS5, miR-146a, IRAK-1, and miR-155 Genetic Variants with Multiple Sclerosis Risk and Severity

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Язык: Английский

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