Expression, Functional Polymorphism, and Diagnostic Values of MIAT rs2331291 and H19 rs217727 Long Non-Coding RNAs in Cerebral Ischemic Stroke Egyptian Patients

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 842 - 842

Опубликована: Янв. 10, 2024

Cerebral ischemic stroke (CIS) is a severe cerebral vascular event. This research aimed to evaluate the role of single-nucleotide polymorphisms (SNPs) lncRNAs MIAT rs2331291 and H19 rs217727 epigenetic methylation in expression patterns serum lncRNA CIS Egyptian patients. It included 80 cases 40 healthy subjects. Serum levels decreased, whereas increased among compared controls. For rs2331291, there were significant differences genotypic allelic frequencies between subjects at p = 0.02 0.0001, respectively. Our findings illustrated significantly T/T genotype frequency hypertensive non-hypertensive 0.004. However, gene C/C was not higher than CIS. The promoter patients level positively correlated with Receiver operating characteristics (ROC) analysis revealed that have high diagnostic potential for distinguishing from ones. In conclusion, MIAT-rs2331291 polymorphism might serve as novel indicator

Язык: Английский

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Repaglinide Elicits a Neuroprotective Effect in Rotenone-Induced Parkinson’s Disease in Rats: Emphasis on Targeting the DREAM-ER Stress BiP/ATF6/CHOP Trajectory and Activation of Mitophagy

ACS Chemical Neuroscience, Год журнала: 2022, Номер 14(1), С. 180 - 194

Опубликована: Дек. 20, 2022

Repaglinide, a meglitinide insulinotropic antidiabetic, was unraveled as promising therapeutic agent for Huntington's disease by targeting the neuronal calcium sensor downstream regulatory element antagonist modulator (DREAM). However, its mechanistic profile in Parkinson's (PD) especially impact on endoplasmic reticulum (ER) stress, mitophagy, and their interconnections is poorly elucidated. This study first to examine neuroprotective potential of repaglinide rotenone-induced PD rats exploring effects DREAM, BiP/ATF6/CHOP ER stress pathway, apoptosis, mitophagy/autophagy, oxidative astrogliosis/microgliosis, neuroinflammation. Male Wistar were randomly assigned four groups: groups 1 2 received vehicle or (0.5 mg/kg/day p.o). Groups 3 4 rotenone (1.5 mg/kg/48 h s.c) 21 days; meanwhile, group additionally p.o) 15 days starting from day 11. Interestingly, lessened striatal apoptosis evidenced reduced caspase-3 levels; however, it augmented DREAM mRNA expression. Repaglinide triggered expression mitophagy marker PINK1 autophagy protein beclin1 alleviated through escalating catalase activity. In addition, halted astrocyte/microglial activation neuroinflammation striatum expressed reducing glial fibrillary acidic (GFAP) ionized calcium-binding adaptor (Iba1) immunostaining decreasing interleukin (IL)-6 IL-1β levels. restored morphological alterations, intact neuron count, neurobehavioral motor performance examined an open field, grip strength, footprint gait analysis. Conclusively, modulates DREAM-ER cascade, increases inhibits lessens activation, PD.

Язык: Английский

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Association of LncRNA-PAX8-AS1 and LAIR-2 polymorphisms along with their expression with clinical and subclinical hypothyroidism

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Янв. 2, 2023

Abstract The genetic and epigenetic architecture of clinical subclinical hypothyroidism remains unclear. We investigated the impact long noncoding RNA (LncRNA)-PAX8-AS1 LAIR-2 variants on susceptibility to hypothyroidism, their influence LncRNA-PAX8-AS1 expression potential as hypothyroid biomarkers. Hundred patients, 110 95 healthy controls were enrolled. Gene analysis genotyping performed by qPCR. protein, a proinflammatory mediator, was tested ELISA. Serum downregulated, whereas mRNA protein levels upregulated in patients compared controls. rs4848320 rs1110839 associated with increased risk hypothyroidism. Interestingly, both SNPs differential serum among . rs2287828 elevated Harboring T allele augmented while patients. rs4848320-rs1110839-rs2287828 TTT, CTT, CGT haplotypes risk. Surprisingly, demonstrated superior diagnostic accuracy for turned out independent predictors multivariate analysis. Conclusively, are novel biomarkers that could alter expression. profiles have effective prognostic indicators

Язык: Английский

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Long Non-Coding RNAs, Novel Offenders or Guardians in Multiple Sclerosis: A Scoping Review

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Дек. 7, 2021

Multiple sclerosis (MS), a chronic inflammatory demyelinating disease of the central nervous system, is one most common neurodegenerative diseases worldwide. MS results in serious neurological dysfunctions and disability. Disturbances coding non-coding genes are key components leading to neurodegeneration along with environmental factors. Long RNAs (lncRNAs) long molecules cells that take part regulation gene expression. Several studies have confirmed role lncRNAs such as MS. In current study, we performed systematic analysis this disorder. total, 53 were recognized eligible for review. Of listed lncRNAs, 52 upregulated, 37 downregulated, 11 had no significant expression difference patients compared controls. We also summarized some mechanisms lncRNA functions The emerging suggests their dysregulation could trigger neuronal death via still unexplored RNA-based regulatory mechanisms. Evaluation diagnostic significance therapeutic potential help design novel treatments

Язык: Английский

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The emerging role of noncoding RNAs in systemic lupus erythematosus: new insights into the master regulators of disease pathogenesis

Therapeutic Advances in Chronic Disease, Год журнала: 2023, Номер 14

Опубликована: Янв. 1, 2023

Auto-immune diseases are a form of chronic disorders in which the immune system destroys body’s cells due to loss tolerance self-antigens. Systemic lupus erythematosus (SLE), identified by production autoantibodies different body parts, is one most well-known examples these diseases. Although etiology SLE unclear, disease’s progression may be affected genetic and environmental factors. As studies twins provide adequate evidence for involvement SLE, other phenomena such as metallization, histone modifications, alterations expression noncoding RNAs (ncRNAs) also indicate epigenetic factors this disease. Among all alterations, ncRNAs appear have crucial contribution pathogenesis SLE. The ncRNAs’ length size divided into three main classes: micro RNAs, long (LncRNA), circular (circRNAs). Accumulating suggests that dysregulations contributed Hence, clarifying function groups pathophysiology provides deeper understanding It opens up new opportunities develop targeted therapies

Язык: Английский

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Serum a proliferation-inducing ligand and MicroRNA-223 are associated with rheumatoid arthritis: diagnostic and prognostic implications

Molecular Medicine, Год журнала: 2020, Номер 26(1)

Опубликована: Окт. 1, 2020

Current blood-based tests for rheumatoid arthritis (RA) have inherent limitations, necessitating the need additional new biomarkers its diagnosis and monitoring disease activity responsiveness to therapy. MicroRNAs (miRNAs) a proliferation-inducing ligand (APRIL) are deregulated in RA were linked pathogenesis. This study investigated serum levels of APRIL, miR-223 miR-155 patients, their potential as diagnostic prognostic biomarkers, correlation with clinicopathological data.One hundred twenty Egyptian patients 130 healthy controls included. Serum miRNAs APRIL assayed by RT-qPCR ELISA, respectively.Serum significantly upregulated, while was unchanged compared controls. discriminated from AUC = 0.85, whereas superiorly distinguished two groups 1 (sensitivity specificity 100% at cutoff> 4.19 ng/ml) receiver-operating-characteristic analysis. significant predictor multivariate logistic regression In group, positively correlated score (DAS28-CRP). expression miR-155, presence subcutaneous nodules. antinuclear antibody titer reverse direction.Our results suggest could serve RA, risk an excellent biomarker activity. Our data be implicated accurate non-invasive prognosis RA.

Язык: Английский

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Long non‐coding RNA growth arrest‐specific 5 and its targets, microRNA‐21 and microRNA‐140, are potential biomarkers of allergic rhinitis

Journal of Clinical Laboratory Analysis, Год журнала: 2021, Номер 35(10)

Опубликована: Сен. 2, 2021

Long non-coding RNA growth arrest-specific 5 (lnc-GAS5) and its targets (microRNA [miR]-21 miR-140) are involved in the development progression of allergic rhinitis (AR). However, correlation lnc-GAS5 with miR-21 miR-140 their associations disease risk, symptom severity, Th1/Th2 cytokines AR remain unclear. Thus, this study aimed to investigate topic.In total, 120 patients 60 controls were recruited. Nasal-mucosa tissues collected from all participants. Lnc-GAS5, (miR-21 miR-140), interferon (IFN)-γ, interleukin (IL)-2, IL-4, IL-10 detected by reverse-transcription quantitative polymerase chain reaction.Lnc-GAS5 was elevated, while downregulated than (p < 0.001). In patients, negatively correlated 0.001), IFN-γ = 0.019), IL-2 0.039) positively IL-4 0.004) individual nasal scores (INSSs) for itching, sneezing, congestion 0.05), total score (TNSS) Moreover, some INSSs, TNSS score, 0.05).Lnc-GAS5 is that AR; meanwhile, lnc-GAS5, miR-21, imbalance AR, suggesting potential these biomarkers AR.

Язык: Английский

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Identification of potential regulatory long non-coding RNA-associated competing endogenous RNA axes in periplaque regions in multiple sclerosis

Frontiers in Genetics, Год журнала: 2022, Номер 13

Опубликована: Окт. 17, 2022

Slow-burning inflammation at the lesion rim is connected to expansion of chronic multiple sclerosis (MS) lesions. However, underlying processes causing are not clearly realized. In this context, current study used a bioinformatics approach identify expression profiles and related lncRNA-associated ceRNA regulatory axes in periplaque region MS patients. Expression data (GSE52139) from regions secondary progressive spinal cord controls were downloaded Gene Omnibus database (GEO), which has details on mRNAs lncRNAs. Using R software’s limma package, differentially expressed lncRNAs (DElncRNAs) (DEmRNAs) found. The RNA interactions also found using DIANA-LncBase, miRTarBase, HMDD databases. Pearson correlation coefficient was determine whether there any positive correlations between DEmRNAs DElncRNAs network. Finally, created based co-expression connections DElncRNA, miRNA, DEmRNA. We Enrichr tool enrich biological process, molecular function, pathways for DElncRNAs. A network DEmRNAs’ protein-protein developed, top five hub genes Cytoscape STRING. indicates that 15 DEmRNAs, including FOS , GJA1 NTRK2 CTNND1 SP3 Additionally, four (such as TUG1 ASB16-AS1 LINC01094 ) regulated aforementioned by sponging 14 MS-related miRNAs (e.g., hsa-miR-145-5p hsa-miR-200a-3p hsa-miR-20a-5p hsa-miR-22-3p hsa-miR-23a-3p hsa-miR-27a-3p hsa-miR-29b-3p hsa-miR-29c-3p hsa-miR-34a-5p addition, analysis pathway enrichment revealed enriched “MAPK signaling pathway”, “Kaposi sarcoma-associated herpesvirus infection”, “Human immunodeficiency virus one “Lipid atherosclerosis”, “Amphetamine addiction”. Even though function these needs be investigated further, provides research targets studying ceRNA-mediated mechanisms demyelination MS.

Язык: Английский

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Diagnostic efficacy of long non-coding RNAs in multiple sclerosis: a systematic review and meta-analysis

Frontiers in Genetics, Год журнала: 2024, Номер 15

Опубликована: Май 15, 2024

Currently, an increasing body of research suggests that blood-based long non-coding RNAs (lncRNAs) could serve as biomarkers for diagnosing multiple sclerosis (MS). This meta-analysis evaluates the diagnostic capabilities selected lncRNAs in distinguishing individuals with MS from healthy controls and differentiating between relapsing remitting phases disease.

Язык: Английский

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Serum lncRNAs TUG1, H19, and NEAT1 and their target miR-29b/SLC3A1 axis as possible biomarkers of preeclampsia: Potential clinical insights

Non-coding RNA Research, Год журнала: 2024, Номер 9(4), С. 995 - 1008

Опубликована: Июнь 13, 2024

To date, the epigenetic signature of preeclampsia (PE) is not completely deciphered. Oxidative stress-responsive long non-coding RNAs (lncRNAs) are deregulated in preeclamptic placenta; however, their circulating profiles and diagnostic abilities still unexplored. We investigated serum redox-sensitive lncRNAs TUG1, H19, NEAT1, target miR-29b/cystine/neutral/dibasic amino acids transporter solute carrier family 3, member 1 (SLC3A1) as potential non-invasive biomarkers PE risk, onset, severity. recruited 82 patients with 78 healthy pregnant women. classified into early-onset (EOPE) late-onset (LOPE) subgroups at a cut-off 34 gestational weeks severe mild by blood pressure proteinuria criteria. Bioinformatics analysis was employed to select lncRNAs/microRNA/target gene interactions. Serum SLC3A1 mRNA expression were reduced, meanwhile miR-29b levels elevated, whereas there no significant difference TUG1 between pregnancies. H19 lower, higher EOPE versus LOPE. PE. ROC identified miR-29b, markers, (AUC = 0.818, 95%CI 0.744–0.894) 0.82, 0.755–0.885) superior discriminators. Only discriminated cases. In multivariate logistic analysis, associated EOPE, using maternal age covariates, while PE, covariate. Studied markers correlated clinical ultrasound data overall group. negatively albuminuria LOPE, respectively. NEAT1 EOPE. Likewise, showed correlations Conclusively, this study configures novel biomarker advocates

Язык: Английский

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