Scavenging the hidden impacts of non-coding RNAs in multiple sclerosis

Non-coding RNA Research, Год журнала: 2021, Номер 6(4), С. 187 - 199

Опубликована: Дек. 1, 2021

Multiple sclerosis (MS) is a chronic neuroinflammatory disease that causes severe neurological dysfunction leading to disabilities in patients. The prevalence of the has been increasing gradually worldwide, and specific etiology behind not yet fully understood. Therapies aimed against treating MS patients have growing lately, intending delay progression increase patients' quality life. Various pathways play crucial roles developing disease, several therapeutic approaches tackling those pathways. However, these strategies shown side effects inconsistent efficacy. MicroRNAs (miRNAs), long noncoding RNAs (lncRNAs), circular (circRNAs) act as key players various pathogenesis development. Several proinflammatory anti-inflammatory miRNAs reported participate development MS. Hence, review assesses role miRNAs, lncRNAs, circRNAs regulating immune cell functions better understand their impact on molecular mechanics

Язык: Английский

---

RETRACTED ARTICLE: GAS5 knockdown alleviates spinal cord injury by reducing VAV1 expression via RNA binding protein CELF2

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Фев. 11, 2021

Abstract Long non-coding RNA growth arrest specific transcript 5 (GAS5) has been found to be implicated in the pathogenesis of central nervous diseases and a contributor hypoxic brain injury. However, roles molecular mechanisms GAS5 spinal cord injury (SCI) have not thoroughly investigated. Here, we reported that knockdown improved rat locomotor function alleviated pathological damage tissues by reducing oxidative stress, caspase-3 activity vav guanine nucleotide exchange factor 1 (VAV1) expression SCI models. inhibited increase malondialdehyde (MDA) level cell apoptotic rate induced oxygen–glucose deprivation (OGD) weakened inhibitory effects OGD on superoxide dismutase (SOD) glutathione peroxidase (GSH-Px) activities viability RN-Sc cells, suggesting loss mitigated OGD-triggered stress cells. Molecular mechanism explorations revealed recruited CUGBP, Elav-like family member 2 (CELF2) coding region VAV1 mRNA, resulting mRNA stability levels. OGD-induced GAS5-induced OGD-treated As conclusion, our findings suggested aggravated increasing via binding with CELF2, deepening understanding basis SCI.

Язык: Английский

---

Meta-Analysis of miRNA Variants Associated with Susceptibility to Autoimmune Disease

Disease Markers, Год журнала: 2021, Номер 2021, С. 1 - 21

Опубликована: Окт. 8, 2021

Purpose. Various studies have shown an association between miRNA polymorphisms and susceptibility to autoimmune disease (AD); however, the results are inconclusive. To evaluate whether account for a significant risk of AD, total 87 articles, including 39431 patients 56708 controls, were identified estimate their with 12 AD subtypes. Methods. Several electronic databases searched analyze population-based on relationship variants risk. Fixed effects or random effect models used in meta-analysis assessment. Results. In our meta-analysis, miR-146a rs2910164/rs57095329 conferred marginally elevated (allele model, OR = 1.08 , 95% CI: 1.01-1.15, P = 0.019 ; allele OR = 1.09 95 1.05-1.15, P < 0.001 respectively). Furthermore, miR-196a2 rs11614913 was also associated OR = 0.92 0.88-0.97, P = 0.001 ) as well miR-499 rs3746444 OR = 1.16 1.03-1.29, P = 0.011 ). addition, associations observed miR-149 rs2292832/miR-27a rs895819 overall population OR = 1.15 1.06-1.24, P < 0.001 OR = 1.11 CI:1.01-1.22, P = 0.043 Conclusions. Evidence from systematic review suggests that miR-146a, miR-196a2, miR-499, miR-149, miR-27a AD.

Язык: Английский

---

Identification of Novel Key Genes and Pathways in Multiple Sclerosis Based on Weighted Gene Coexpression Network Analysis and Long Noncoding RNA-Associated Competing Endogenous RNA Network

Oxidative Medicine and Cellular Longevity, Год журнала: 2022, Номер 2022, С. 1 - 19

Опубликована: Март 2, 2022

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system characterized by chronic inflammation and demyelination. This study aimed at identifying crucial genes molecular pathways involved in MS pathogenesis. Raw data GSE52139 were collected from Gene Expression Omnibus. The top 50% expression variants subjected to weighted gene coexpression network analysis (WGCNA), key module associated with occurrence was identified. A long noncoding RNA- (lncRNA-) competing endogenous RNA (ceRNA) constructed module. hub candidates subsequently verified individual database. Of 18 modules obtained, cyan designated as established ceRNA composed seven lncRNAs, 45 mRNAs, 21 microRNAs (miRNAs), FAM13A-AS1 lncRNA highest centrality. Functional assessments indicated that primarily gathered ribosome-related functional terms. Interestingly, targeted mRNAs enriched diverse categories. Moreover, highly expressed CYBRD1, GNG12, SMAD1, which identified genes, may be "valine leucine isoleucine degradation," "base excision repair," "fatty acid metabolism," respectively, according results single gene-based genomes set enrichment (GSEA). Combined WGCNA network, our findings provide novel insights into pathogenesis MS. discovered herein might also serve biomarkers correlate development management

Язык: Английский

---

Long noncoding RNA GAS5 and miR-137 and two of their genetic polymorphisms contribute to acute ischemic stroke risk in an Egyptian population

Journal of the Pakistan Medical Association, Год журнала: 2023, Номер 73(4), С. S184 - S190

Опубликована: Май 25, 2023

To assess the serum expression levels of long non-coding ribonucleic acid growth arrest specific-5 and micro-ribonucleic acid-137, different genotypes arrestspecific-5 rs2067079 (C>T) acid-137 rs1625579 (T>G) in acute ischaemic stroke patients.The case-control study was conducted at Cairo University, Cairo, Egypt, from January to August 2020, comprised adult patients either gender selected unit Neurology Department Kasr Alainy Hospital University. Healthy individuals matched for age were enrolled as controls. Quantitative real-time polymerase chain reaction used quantify genotype non coding lncRNA using TaqMan allelic discrimination. Data analysed SPSS 22.Of 100 subjects, 50(50%) patients; 34(68%) males 16(32%) females with mean 60.4±10.0 years. The remaining controls; 28(56%) 22(44%) 56.9±12.2 years (p>0.05). had more smokers, hypertensives diabetics than controls (p<0.05). Long significantly increased, while microribonucleic reduced among patients(p<0.05). Acute risk higher recessive model (homozygous minor TT genotype), protective against (G allele), codominant (GT dominant (GT+GG), over-dominant genotype) models (p<0.05).Long may act novel genetic markers risk.

Язык: Английский

---