Quantum Dots for Chemical Metrology

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

Язык: Английский

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Machine learning and statistical classification in CRISPR-Cas12a diagnostic assays

Biosensors and Bioelectronics, Год журнала: 2025, Номер 279, С. 117402 - 117402

Опубликована: Март 31, 2025

CRISPR-based diagnostics have gained increasing attention as biosensing tools able to address limitations in contemporary molecular diagnostic tests. To maximize the performance of assays, much effort has focused on optimizing chemistry and biology reaction. However, less been paid improving techniques used analyze data. date, decisions typically involve various forms slope-based classification. Such methods are superior traditional based assessing absolute signals, but still limitations. Herein, we establish benchmarks (total accuracy, sensitivity, specificity) using common methods. We compare these benchmark with three different quadratic empirical distribution function statistical tests, finding significant improvements speed accuracy when applied a clinical data set. Two techniques, Kolmogorov-Smirnov Anderson-Darling report lowest time-to-result highest total test accuracy. Furthermore, developed long short-term memory recurrent neural network classify CRISPR-biosensing data, achieving 100 % specificity our model Finally, provide guidelines choosing classification method parameters that best suit assay's needs.

Язык: Английский

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Nanomaterials-Integrated CRISPR/Cas Systems: Expanding the Toolbox for Optical Detection

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Nanomaterials-integrated CRISPR/Cas systems have rapidly emerged as powerful next-generation platforms for optical biosensing. These integrated harness the precision of CRISPR/Cas-mediated nucleic acid detection while leveraging unique properties nanomaterials to achieve enhanced sensitivity and expanded analytical capabilities, thereby broadening their diagnostic potential. By incorporating a diverse range nanomaterials, these effectively expand toolbox detection, offering adaptable solutions tailored various challenges. This review provides comprehensive overview successfully into CRISPR/Cas-based sensing systems. It examines multiple modalities, including fluorescence, electrochemiluminescence, colorimetry, surface-enhanced Raman spectroscopy, highlighting how facilitate signal amplification, enable multiplexing, support development point-of-care applications. Additionally, practical applications in critical fields such healthcare diagnostics environmental monitoring are showcased. While offer considerable advantages, several real-world challenges complexity assay workflows, impact cost, regulatory hurdles must be addressed before widespread implementation can achieved. identifying obstacles proposing strategic solutions, we aim pave way continued advancement adoption nanomaterial-integrated biosensing technologies.

Язык: Английский

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CRISPR/Cas Multiplexed Biosensing: Advances, Challenges, and Perspectives

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 27, 2025

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein systems are renowned for their high sensitivity specificity, enabling them as a powerful diagnostic toolbox. Multiplexed detection of panels targets, opposed to single is imperative reliable conclusive disease diagnostics. However, multiplex application the CRISPR/Cas system has long been hindered by indistinguishable signals from specific targets due nonspecific chaotic trans-cleavage. To make breakthrough, substantial efforts have devoted CRISPR/Cas-powered multiplexed biosensing strategies, which consequently experienced rapid development over past five years. This review systematically summarizes recent advances in encompassing Cas9, Cas12, Cas13. Key focus issues include strategies respective advantages limitations, sensing mechanisms, performance novel validated examples. Finally, status challenges critically discussed, future outlooks proposed potential practical application. Perspective aims inspire significant research promote next generation biosensing.

Язык: Английский

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Multiplexed CRISPR/Cas and Argonaute detection technology: strategies, challenges, and perspectives

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118329 - 118329

Опубликована: Июнь 1, 2025

Язык: Английский

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