Fluorescence coding techniques for RNA detection DOI Creative Commons

Junren Wang,

Qin Xiang, Haifeng Dong

et al.

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(6), P. 914 - 932

Published: Jan. 1, 2024

Schematic illustration of fluorescence coding techniques in RNA detection.

Language: Английский

Fluorescent and colorimetric dual-readout platform for tuberculosis point-of-care detection based on dual signal amplification strategy and quantum dot nanoprobe DOI

Ou Hu,

Yingyu Gong,

Yuexiang Chang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116641 - 116641

Published: Aug. 8, 2024

Language: Английский

Citations

3
Quantum Dot Reporters Designed for CRISPR-Based Detection of Viral Nucleic Acids DOI

Ziwen Tang,

Menglu Gao, Feng Gong

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(40), P. 16017 - 16026

Published: Sept. 26, 2024

Diagnostic methods based on CRISPR technology have shown great potential due to their highly specific, efficient, and sensitive detection capabilities. Although the majority of current studies rely fluorescent dye-quencher reporters, limitations dyes, such as poor photostability small Stokes shifts, urgently necessitate optimization reporters. In this study, we developed innovative quantum dot (QD) reporters for CRISPR/Cas systems, which not only leveraged advantages high photoluminescence yield large shifts QDs but were also easily synthesized through a simple one-step hydrothermal method. Based trans-cleavage characteristics Cas12a Cas13a, two types QD designed, short DNA strand hybridization-based achieving RNA at pM level, respectively, validating performance in analysis clinical samples. Furthermore, unique property that allowed multicolor emission under one excitation, application simultaneous diseases was further investigated. Taken together, work proposed novel could be applied various providing new toolbox expand diagnosis bioanalytical biomedical fields.

Language: Английский

Citations

3
Recent advances in the CRISPR/Cas system-based visual detection method DOI
Jinrong Chen, Hang Su, June Hyun Kim

et al.

Analytical Methods, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Reviews the development of visual detection methods in recent years based on different Cas and analyzes their advantages disadvantages as well challenges future research.

Language: Английский

Citations

3
Amplification-free miRNA detection with CRISPR/Cas12a System based on Fragment Complementary Activation Strategy DOI Creative Commons
Shuang Zhao,

Qiuting Zhang,

Ran Luo

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: 15(44), P. 18347 - 18354

Published: Jan. 1, 2024

CRISPR/Cas12a systems have been repurposed as powerful tools for developing next-generation molecular diagnostics due to their

Language: Английский

Citations

3
Fluorescence coding techniques for RNA detection DOI Creative Commons

Junren Wang,

Qin Xiang, Haifeng Dong

et al.

Sensors & Diagnostics, Journal Year: 2024, Volume and Issue: 3(6), P. 914 - 932

Published: Jan. 1, 2024

Schematic illustration of fluorescence coding techniques in RNA detection.

Language: Английский

Citations

2