Biosensors,
Год журнала:
2024,
Номер
14(12), С. 625 - 625
Опубликована: Дек. 18, 2024
Metal
nanoclusters
(NCs)
are
promising
alternatives
to
organic
dyes
and
quantum
dots.
These
NCs
exhibit
unique
physical
chemical
properties,
such
as
fluorescence,
chirality,
magnetism
catalysis,
which
contribute
significant
advancements
in
biosensing,
biomedical
diagnostics
therapy.
Through
adjustments
composition,
size,
environments
surface
ligands,
it
is
possible
create
with
tunable
optoelectronic
catalytic
activity.
This
review
focuses
on
the
integration
of
aptamers
metal
NCs,
detailing
molecular
detection
strategies
that
utilise
effect
optical
signal
emission
NC-based
biosensing
systems.
also
highlights
recent
applications,
well
illustrative
case
studies.
To
conclude,
strengths,
limitations,
current
challenges
prospects
for
systems
were
examined.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(30), С. 16771 - 16777
Опубликована: Июль 13, 2023
DNA-stabilized
silver
nanoclusters
(DNA-AgNCs)
are
biocompatible
emitters
with
intriguing
properties.
However,
they
have
not
been
extensively
used
for
bioimaging
applications
due
to
the
lack
of
structural
information
and
hence
predictable
conjugation
strategies.
Here,
a
copper-free
click
chemistry
method
linking
well-characterized
DNA-AgNC
molecules
interest
is
presented.
Three
different
peptides
small
protein,
human
insulin,
were
tested
as
labeling
targets.
The
target
compounds
was
verified
by
MS,
HPLC,
time-resolved
anisotropy
measurements.
Moreover,
spectroscopic
properties
DNA-AgNCs
found
be
unaffected
reactions.
For
DNA-AgNC-conjugated
fluorescence
imaging
studies
performed
on
Chinese
hamster
ovary
(CHO)
cells
overexpressing
insulin
receptor
B
(hIR-B).
specific
staining
CHO
cell
membranes
demonstrates
that
great
candidates
applications,
proposed
strategy
easy
implement
when
structure
known.
Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Март 26, 2024
Abstract
For
generations
researchers
have
been
observing
the
dynamic
processes
of
life
through
lens
a
microscope.
This
has
offered
tremendous
insights
into
biological
phenomena
that
span
multiple
orders
time-
and
length-scales
ranging
from
pure
magic
molecular
reorganization
at
membrane
immune
cells,
to
cell
migration
differentiation
during
development
or
wound
healing.
Standard
fluorescence
microscopy
techniques
offer
glimpses
such
in
vitro,
however,
when
applied
intact
systems,
they
are
challenged
by
reduced
signal
strengths
signal-to-noise
ratios
result
deeper
imaging.
As
remedy,
two-photon
excitation
(TPE)
takes
special
place,
because
it
allows
us
investigate
vivo,
their
natural
environment,
even
living
animal.
Here,
we
review
fundamental
principles
underlying
TPE
aimed
basic
advanced
users
interested
adopting
for
intravital
We
focus
on
applications
neurobiology,
present
current
trends
towards
faster,
wider
imaging,
discuss
combination
with
photon
counting
technologies
metabolic
imaging
spectroscopy,
as
well
highlight
outstanding
issues
drawbacks
application
these
methodologies.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(8), С. 3480 - 3488
Опубликована: Фев. 14, 2024
To
address
the
limitations
of
typical
hairpin-structural
molecular
beacons,
exploring
ability
a
quasi-molecular
beacon
(qMB)
to
create
label-free
fluorescence
biosensors
is
intriguing
and
remains
challenge.
Herein,
we
propose
first
example
modular
qMB
with
feature
stimulation-responsive
conformation
switch
develop
an
aggregated
Ag
nanocluster
(aAgNC)
in
bifurcated
DNA
scaffold
for
fluorescently
sensing
specific
initiator
(I*).
This
was
well
designed
program
four
functional
modules:
I*-recognizable
element
adopting
metastable
stem-loop
bihairpin
structure
two
splits
(exposed
C3GT4
locked
C4AC4T)
aAgNC
template
that
separated
by
tunable
hairpin
spacer
customized
combination
selective
recognition
signaling
readout.
When
presenting
I*
assay
route,
hybridization
induces
directional
disassembly
unit,
on
which
configurationally
switched
liberate
split.
Thus,
parent
pair
C3GT4/C4AC4T
proximal,
affording
situ
nucleation
clustering
emissive
aAgNC.
By
collecting
signal,
quantitative
detection
achieved.
Benefiting
from
ingenious
programming
qMB,
recognizing
integration
actuates
construction
facile
convenient
fluorescent
biosensor
featuring
rapid
reaction
kinetics,
wide
linear
range,
high
sensitivity,
specificity.
would
provide
new
paradigm
exploit
versatile
qMB-based
biosensing
platforms
via
switches
developing
various
DNA-scaffolded
clusters.
ABSTRACT
Hydrogels
loaded
with
microRNA
(miRNA)
have
shown
promise
in
bone‐defect
repair.
Here,
we
present
the
first
report
of
miRNA‐loaded
hydrogels
containing
bioactivities
to
treat
steroid‐induced
osteonecrosis
femoral
head
(SONFH),
based
on
mechanism
competing
endogenous
RNAs.
Transcriptome
sequencing
human
bone
marrow
mesenchymal
stem
cells
(HBMSCs)
extracted
from
proximal
and
subsequent
functional
assays
revealed
that
circSRPK1/miR‐320a
axis
promotes
HBMSCs
osteogenic
differentiation.
By
incorporating
antagomir‐320a
(a
miR‐320a
inhibitor)
encapsulated
liposomes
into
injectable
hyaluronic
acid
(HA)
hydrogels,
constructed
an
hydrogel,
HA@antagomir‐320a.
This
hydrogel
demonstrated
exceptional
properties,
targeting
multiple
pathways
via
CDH2
Osterix
exhibited
excellent
vitro
biocompatibility.
In
vivo,
it
substantially
enhanced
formation
osteonecrotic
area
head.
HA@antagomir‐320a
which
biocompatibility
properties
vivo
vitro,
offers
a
promising
minimally
invasive
solution
for
treatment
SONFH.
DNA-stabilized
silver
nanoclusters
(DNA-AgNCs)
are
a
class
of
fluorophores
with
interesting
photophysical
properties.
They
capable
generating
anti-Stokes
fluorescence
upon
excitation
near-infrared
lasers.
The
has
previously
been
speculated
to
be
either
the
result
consecutive
photon
absorption
(upconversion)
or
hot
band
(HBA).
Here,
we
revisit
DNA-AgNCs
determine
underlying
mechanistic
origin.
We
investigate
two
studied
together
organic
and
emission
features
in
same
spectral
regions
as
DNA-AgNCs.
From
recorded
spectra,
find
that
all
emitters
exhibit
an
exponential-like
decaying
slope
on
red
side
lowest
energy
band.
Furthermore,
power
dependency
measurements
at
different
wavelengths
verify
one-photon
nature
fluorescence.
Ultimately,
lack
discrete
optical
transition
spectra
suggests
HBA
mechanism
is
most
plausible
cause
for
investigated
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 28, 2025
It
might
be
intriguing
to
explore
the
tailored
ability
of
a
bihairpin-structured
molecular
beacon
(bhMB)
for
improving
reaction
kinetics
and
assay
sensitivity
label-free
ratiometric
fluorescence
specific
trigger
(T*),
in
which
red
green
silver
nanoclusters
(rSNC
gSNC)
were
utilized
as
two
reverse
emitting
reporters.
Unlike
traditional
dually
labeled
MB,
herein
proposed
bhMB
was
designed
with
hairpin
subunits
recognition
signaling
readout.
Four
functional
modules
included:
complement
T*
identifiable
hairpin,
template
sequence
rSNC
gSNC
variable
consensus
loops
capable
complementarily
hybridizing
form
one
paired
stem,
stems
able
merge
into
new
loop.
Upon
introducing
T*,
conformation
switch
triggered
via
affinity
linking
cognate
sequence,
thereby
reconstructing
perfect
stem–loop
monohairpin
structure.
As
such,
mutual
transition
between
nonbinding
binding
states
powered
by
population-shift
mechanism
shift
thermodynamic
equilibrium
toward
state.
Benefiting
from
structure
confinement
bhMB,
local
concentration
reactive
species
increased
speed
up
kinetics.
In
response
route,
clustering
merged
fluorescence,
while
emission
decreased,
pointing
obtained
sensitive
signal
built-in
correction.
Thus,
this
strategy
would
first
example
integrate
recognizing
double-hairpin
biosensing
potential
applications.
Near-infrared
(NIR)
emitters
with
high
two-photon
absorption
(2PA)
cross-sections
are
of
interest
to
enable
in
vivo
imaging
the
tissue
transparency
windows.
This
study
explores
potential
DNA-stabilized
silver
nanoclusters
(AgN-DNAs)
as
water-soluble
absorbers.
We
investigate
2PA
four
different
atomically
precise
AgN-DNA
species
far-red
NIR
emission
and
varying
nanocluster
ligand
compositions.
cross-sections,
σ2,
were
determined
by
excited
luminescence
(TPEL)
technique
for
a
wide
wavelength
range
from
810
1400
nm.
The
AgN-DNAs
exhibited
reversed
strength
corresponding
transitions
regime,
compared
one-photon,
which
adds
another
puzzle
complex
photophysics
these
emitters.
Maximal
cross-section
value
(813
GM)
was
observed
(DNA)3[Ag21]15+,
is
stabilized
3
DNA
oligomers.
(DNA)2[Ag16Cl2]8+
presented
distinct
behavior
without
chlorido
ligands,
presenting
176
GM
at
1050
Our
findings
support
NIR-to-NIR
probes
that
both
emit
NIR.
Their
σ2
fluorescence
quantum
yield
results
superior
brightness
on
order
~10^2
GM,
significantly
higher
than
organic
fluorophores.