Bioconjugation of a Near-Infrared DNA-Stabilized Silver Nanocluster to Peptides and Human Insulin by Copper-Free Click Chemistry

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(30), С. 16771 - 16777

Опубликована: Июль 13, 2023

DNA-stabilized silver nanoclusters (DNA-AgNCs) are biocompatible emitters with intriguing properties. However, they have not been extensively used for bioimaging applications due to the lack of structural information and hence predictable conjugation strategies. Here, a copper-free click chemistry method linking well-characterized DNA-AgNC molecules interest is presented. Three different peptides small protein, human insulin, were tested as labeling targets. The target compounds was verified by MS, HPLC, time-resolved anisotropy measurements. Moreover, spectroscopic properties DNA-AgNCs found be unaffected reactions. For DNA-AgNC-conjugated fluorescence imaging studies performed on Chinese hamster ovary (CHO) cells overexpressing insulin receptor B (hIR-B). specific staining CHO cell membranes demonstrates that great candidates applications, proposed strategy easy implement when structure known.

Язык: Английский

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More than double the fun with two-photon excitation microscopy

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Март 26, 2024

Abstract For generations researchers have been observing the dynamic processes of life through lens a microscope. This has offered tremendous insights into biological phenomena that span multiple orders time- and length-scales ranging from pure magic molecular reorganization at membrane immune cells, to cell migration differentiation during development or wound healing. Standard fluorescence microscopy techniques offer glimpses such in vitro, however, when applied intact systems, they are challenged by reduced signal strengths signal-to-noise ratios result deeper imaging. As remedy, two-photon excitation (TPE) takes special place, because it allows us investigate vivo, their natural environment, even living animal. Here, we review fundamental principles underlying TPE aimed basic advanced users interested adopting for intravital We focus on applications neurobiology, present current trends towards faster, wider imaging, discuss combination with photon counting technologies metabolic imaging spectroscopy, as well highlight outstanding issues drawbacks application these methodologies.

Язык: Английский

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Fluorescence Biosensing Based on Bifurcated DNA Scaffold-Aggregated Ag Nanocluster via Responsive Conformation Switch of Quasi-Molecular Beacon

Analytical Chemistry, Год журнала: 2024, Номер 96(8), С. 3480 - 3488

Опубликована: Фев. 14, 2024

To address the limitations of typical hairpin-structural molecular beacons, exploring ability a quasi-molecular beacon (qMB) to create label-free fluorescence biosensors is intriguing and remains challenge. Herein, we propose first example modular qMB with feature stimulation-responsive conformation switch develop an aggregated Ag nanocluster (aAgNC) in bifurcated DNA scaffold for fluorescently sensing specific initiator (I*). This was well designed program four functional modules: I*-recognizable element adopting metastable stem-loop bihairpin structure two splits (exposed C3GT4 locked C4AC4T) aAgNC template that separated by tunable hairpin spacer customized combination selective recognition signaling readout. When presenting I* assay route, hybridization induces directional disassembly unit, on which configurationally switched liberate split. Thus, parent pair C3GT4/C4AC4T proximal, affording situ nucleation clustering emissive aAgNC. By collecting signal, quantitative detection achieved. Benefiting from ingenious programming qMB, recognizing integration actuates construction facile convenient fluorescent biosensor featuring rapid reaction kinetics, wide linear range, high sensitivity, specificity. would provide new paradigm exploit versatile qMB-based biosensing platforms via switches developing various DNA-scaffolded clusters.

Язык: Английский

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Beyond nature's base pairs: machine learning-enabled design of DNA-stabilized silver nanoclusters

Chemical Communications, Год журнала: 2023, Номер 59(69), С. 10360 - 10375

Опубликована: Янв. 1, 2023

Data mining and machine learning are powerful tools for investigation design of DNA-based nanomaterials.

Язык: Английский

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Multi-Objective Design of DNA-Stabilized Nanoclusters Using Variational Autoencoders With Automatic Feature Extraction

ACS Nano, Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

DNA-stabilized silver nanoclusters (Ag

Язык: Английский

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An Atom-Precise Understanding of DNA-Stabilized Silver Nanoclusters

Accounts of Chemical Research, Год журнала: 2024, Номер 57(15), С. 2117 - 2129

Опубликована: Июль 12, 2024

ConspectusDNA-stabilized silver nanoclusters (Ag

Язык: Английский

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Functional Injectable Hydrogel for Bone Regeneration: Regulation of the circSRPK1/miR‐320a Axis and Targeting Multiple Osteogenic Pathways via CDH2 and Osterix Genes

SmartMat, Год журнала: 2025, Номер 6(1)

Опубликована: Янв. 9, 2025

ABSTRACT Hydrogels loaded with microRNA (miRNA) have shown promise in bone‐defect repair. Here, we present the first report of miRNA‐loaded hydrogels containing bioactivities to treat steroid‐induced osteonecrosis femoral head (SONFH), based on mechanism competing endogenous RNAs. Transcriptome sequencing human bone marrow mesenchymal stem cells (HBMSCs) extracted from proximal and subsequent functional assays revealed that circSRPK1/miR‐320a axis promotes HBMSCs osteogenic differentiation. By incorporating antagomir‐320a (a miR‐320a inhibitor) encapsulated liposomes into injectable hyaluronic acid (HA) hydrogels, constructed an hydrogel, HA@antagomir‐320a. This hydrogel demonstrated exceptional properties, targeting multiple pathways via CDH2 Osterix exhibited excellent vitro biocompatibility. In vivo, it substantially enhanced formation osteonecrotic area head. HA@antagomir‐320a which biocompatibility properties vivo vitro, offers a promising minimally invasive solution for treatment SONFH.

Язык: Английский

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Revisiting Anti-Stokes Emission Features of DNA-Stabilized Silver Nanoclusters

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

DNA-stabilized silver nanoclusters (DNA-AgNCs) are a class of fluorophores with interesting photophysical properties. They capable generating anti-Stokes fluorescence upon excitation near-infrared lasers. The has previously been speculated to be either the result consecutive photon absorption (upconversion) or hot band (HBA). Here, we revisit DNA-AgNCs determine underlying mechanistic origin. We investigate two studied together organic and emission features in same spectral regions as DNA-AgNCs. From recorded spectra, find that all emitters exhibit an exponential-like decaying slope on red side lowest energy band. Furthermore, power dependency measurements at different wavelengths verify one-photon nature fluorescence. Ultimately, lack discrete optical transition spectra suggests HBA mechanism is most plausible cause for investigated

Язык: Английский

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Target-Responsive Conformation Switch of Bihairpin Molecular Beacon to Illustrate Ratiometric Fluorescence Biosensing of Dual-Emissive Silver Nanoclusters

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

It might be intriguing to explore the tailored ability of a bihairpin-structured molecular beacon (bhMB) for improving reaction kinetics and assay sensitivity label-free ratiometric fluorescence specific trigger (T*), in which red green silver nanoclusters (rSNC gSNC) were utilized as two reverse emitting reporters. Unlike traditional dually labeled MB, herein proposed bhMB was designed with hairpin subunits recognition signaling readout. Four functional modules included: complement T* identifiable hairpin, template sequence rSNC gSNC variable consensus loops capable complementarily hybridizing form one paired stem, stems able merge into new loop. Upon introducing T*, conformation switch triggered via affinity linking cognate sequence, thereby reconstructing perfect stem–loop monohairpin structure. As such, mutual transition between nonbinding binding states powered by population-shift mechanism shift thermodynamic equilibrium toward state. Benefiting from structure confinement bhMB, local concentration reactive species increased speed up kinetics. In response route, clustering merged fluorescence, while emission decreased, pointing obtained sensitive signal built-in correction. Thus, this strategy would first example integrate recognizing double-hairpin biosensing potential applications.

Язык: Английский

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Two-photon brightness of NIR-emitting, atomically precise DNA-stabilized silver nanoclusters

Опубликована: Июнь 7, 2024

Near-infrared (NIR) emitters with high two-photon absorption (2PA) cross-sections are of interest to enable in vivo imaging the tissue transparency windows. This study explores potential DNA-stabilized silver nanoclusters (AgN-DNAs) as water-soluble absorbers. We investigate 2PA four different atomically precise AgN-DNA species far-red NIR emission and varying nanocluster ligand compositions. cross-sections, σ2, were determined by excited luminescence (TPEL) technique for a wide wavelength range from 810 1400 nm. The AgN-DNAs exhibited reversed strength corresponding transitions regime, compared one-photon, which adds another puzzle complex photophysics these emitters. Maximal cross-section value (813 GM) was observed (DNA)3[Ag21]15+, is stabilized 3 DNA oligomers. (DNA)2[Ag16Cl2]8+ presented distinct behavior without chlorido ligands, presenting 176 GM at 1050 Our findings support NIR-to-NIR probes that both emit NIR. Their σ2 fluorescence quantum yield results superior brightness on order ~10^2 GM, significantly higher than organic fluorophores.

Язык: Английский

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