Target-Navigated CBT–Cys “Stapling” Coupled with CRISPR/Cas12a Amplification for the Photoelectrochemical Nucleic Acid Assay

Analytical Chemistry, Год журнала: 2024, Номер 96(45), С. 18011 - 18019

Опубликована: Сен. 27, 2024

Generally, rolling circle amplification (RCA) is based on an enzyme-linked padlock extension reaction. Herein, rapid linking that utilizes click chemistry for joining sticky ends of DNA molecules was developed. The nucleic acid were modified with 2-cyano-6-aminobenzothiazole (CBT) and cystine (Cys-Cys), while glutathione introduced to break the disulfide bond under target navigation promote linkage between CBT Cys at terminus pH 7.4. Subsequently, RCA performed using phi29 polymerase. CRISPR/Cas12a cleavage triggered by product amplification. Assisted alkaline phosphatase, electron exchange process photoelectroactive Sb@Co(OH)F nanorod

Язык: Английский

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Proximity-Guaranteed DNA Machine for Accurate Identification of Breast Cancer Extracellular Vesicles

ACS Sensors, Год журнала: 2024, Номер 9(4), С. 2194 - 2202

Опубликована: Апрель 15, 2024

Breast cancer is one of the most diagnosed cancers worldwide. Precise diagnosis and subtyping have important significance for targeted therapy prognosis prediction breast cancer. Herein, we design a proximity-guaranteed DNA machine accurate identification extracellular vesicles (EVs), which beneficial to explore subtype features In our design, two proximity probes are located close on same EV through specific recognition coexisting surface biomarkers, thus being ligated with help click chemistry. Then, product initiates operation involving catalytic hairpin assembly clusters regularly interspaced short palindromic repeats (CRISPR)-Cas12a-mediated trans-cleavage, finally generates significant response that enables EVs expressing both biomarkers. Principle-of-proof studies performed using derived from cell line BT474 as models, confirming high sensitivity specificity machine. When further applied clinical samples, shown be capable not only distinguishing patients special subtypes but also realizing tumor staging regarding disease progression. Therefore, work may provide new insights into subtype-based well more potential therapeutic targets in future.

Язык: Английский

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A Review of Click Chemistry in the Synthesis of Organophosphorus Triazoles and Their Biological Activities

European Journal of Medicinal Chemistry, Год журнала: 2025, Номер 286, С. 117270 - 117270

Опубликована: Янв. 13, 2025

Organophosphorus compounds, characterized by the incorporation of phosphorus into organic molecules, play a critical role in various fields such as medicine, agriculture, and industry. Their unique electronic properties versatility make them essential developing therapeutic agents, pesticides, materials. One prominent class organophosphorus compounds is heterocycles, which combine benefits both cyclic structures. Triazoles, nitrogen-containing heterocyclic are particularly notable for their broad biological activities, including anticancer, antiviral, antibacterial, antioxidant effects. Traditional methods synthesizing triazoles often encounter challenges low yields non-selective products, whereas click chemistry provides more efficient reliable alternative. The copper-catalyzed azide-alkyne [3 + 2] cycloaddition, cornerstone chemistry, allows rapid selective formation under mild conditions. When functionalized with groups, not only retain but enhance improving potency, selectivity, stability. This review covers synthesis organophosphorus-functionalized via explores molecular structure, coordination these compounds. behavior interactions derivatives metal ions also addressed, significantly influence chemical reactivity, stability, bioactivity.

Язык: Английский

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Mercaptan Ligand Effect in Determining the Geometric Structures of Silver Nanoclusters

Journal of Cluster Science, Год журнала: 2025, Номер 36(2)

Опубликована: Фев. 9, 2025

Язык: Английский

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Recent progress in atomically precise metal nanoclusters: From bio-related properties to biological applications

Coordination Chemistry Reviews, Год журнала: 2025, Номер 535, С. 216633 - 216633

Опубликована: Март 30, 2025

Язык: Английский

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Beyond nature's base pairs: machine learning-enabled design of DNA-stabilized silver nanoclusters

Chemical Communications, Год журнала: 2023, Номер 59(69), С. 10360 - 10375

Опубликована: Янв. 1, 2023

Data mining and machine learning are powerful tools for investigation design of DNA-based nanomaterials.

Язык: Английский

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A DNA‐Stabilized Ag₁₈¹²⁺ Cluster with Excitation‐Intensity‐Dependent Dual Emission

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(39)

Опубликована: Авг. 14, 2023

DNA-stabilized silver nanoclusters (DNA-AgNCs) are easily tunable emitters with intriguing photophysical properties. Here, a DNA-AgNC dual emission in the red and near-infrared (NIR) regions is presented. Mass spectrometry data showed that two DNA strands stabilize 18 atoms nanocluster charge of 12+. Besides determining composition DNA2 [Ag18 ]12+ , steady-state time-resolved methods were applied to characterize picosecond fluorescence relatively intense microsecond-lived NIR luminescence. During this process, luminescence-to-fluorescence ratio was found be excitation-intensity-dependent. This peculiar feature very rare for molecular allows use as nanoscale excitation intensity probe. For purpose, calibration curves constructed using three different approaches based either on or measurements. The results processes like thermally activated delayed (TADF) photon upconversion through triplet-triplet annihilation (TTA) could excluded . We, therefore, speculate ratiometric response result optically fluorescence.

Язык: Английский

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Leveraging Concentration Imbalance-Driven DNA Circuit as an Operational Amplifier to Enhance the Sensitivity of Hepatitis B Virus DNA Detection with Hybridization-Responsive DNA-Templated Silver Nanoclusters

JACS Au, Год журнала: 2024, Номер 4(6), С. 2323 - 2334

Опубликована: Май 31, 2024

Hepatitis B virus (HBV) infection remains a major global health concern, necessitating the development of sensitive and reliable diagnostic methods. In this study, we propose novel approach to enhance sensitivity HBV DNA detection by leveraging concentration imbalance-driven circuit (CIDDC) as an operational amplifier, coupled with hybridization-responsive DNA-templated silver nanocluster (DNA-AgNCs) nanoprobe named Q·C6-AgNCs. The CIDDC system effectively converts amplifies input into enriched generic single-stranded output, which subsequently triggers fluorescence DNA-AgNCs reporter upon hybridization, generating measurable signal for detection. By incorporating circuit, not only achieved enhanced lower limit 0.11 nM but also demonstrated high specificity single-base mismatch discriminability Additionally, mix-and-detect assay format is simple, user-friendly, isothermal. This innovative strategy holds promise advancing molecular diagnostics facilitating effective management HBV-related diseases.

Язык: Английский

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An Atom-Precise Understanding of DNA-Stabilized Silver Nanoclusters

Accounts of Chemical Research, Год журнала: 2024, Номер 57(15), С. 2117 - 2129

Опубликована: Июль 12, 2024

ConspectusDNA-stabilized silver nanoclusters (Ag

Язык: Английский

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High-Efficient Electrochemiluminescence of DNA-Au Ag Nanoclusters with Au NPs@Ti₃C₂ as a Novel Coreaction Accelerator for Ultrasensitive Biosensing

Analytical Chemistry, Год журнала: 2024, Номер 96(33), С. 13727 - 13733

Опубликована: Авг. 7, 2024

In this work, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed based on DNA-stabilized Au Ag nanoclusters (DNA-Au NCs) as the efficient luminophore and NPs@Ti

Язык: Английский

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