Enhancing the Peroxidase-Mimicking Activity of Gold Nanoparticles for Lateral Flow Assays: Quantitative Evaluation in a Kinetic View

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Highly sensitive lateral flow immunoassays (LFIAs) are essential for various point-of-care applications, and gold nanoparticles (Au NPs) by far the most commonly used labels. However, conventional LFIAs often suffer from high detection limits (LOD) or low sensitivity. In this study, we investigated three strategies to enhance sensitivity of improving peroxidase-mimicking (POD) activity Au NPs. The POD unmodified NPs was negligible (<0.01 units/mg, U/mg). first strategy involved coupling with horseradish peroxidase (HRP), which increased 65 U/mg. second approach forming a thin palladium iridium shell on NPs, elevated 0.69-0.71 third binding mercury ions (Hg2+) resulting in up 3 Finally, developed simple quantitative model estimate LOD based kinetic parameters. Using Au-HRP conjugates, demonstrated that experimentally measured consistent calculated values. provides framework evaluating catalytic signal amplification can be guide development highly assays.

Язык: Английский

---

Improving Nanozyme-Based Colorimetric Assays through Medium Composition Optimization in Nanozyme-Substrate Reaction

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Nanozymes, while promising alternatives to natural peroxidases in colorimetric assays, are often hindered by lower catalytic efficiencies. Although numerous approaches have been developed improve signal intensity nanozyme-based optimization of the reaction medium which nanozyme interacts with substrate remains a significantly underexplored area. The vast majority studies rely on standard sodium acetate buffers or commercially sourced reagents optimized for horseradish peroxidase, neglecting unique properties different nanozymes. This work presents systematic 3,3',5,5'-tetramethylbenzidine (TMB)-based composition four common nanozymes: iron oxide, LaNiO3, Mn-doped CeO2, and platinum nanoparticles. Our findings reveal that buffer is suitable alternative enhance (41-68%) other Further ionic strength, organic cosolvent type concentration, TMB/H2O2 concentrations yielded improvements intensity, analytical sensitivity, assay time. study also identifies pitfalls encountered during conditions proposes potential solutions. We posit should be step development use undisclosed compositions avoided.

Язык: Английский

---

Construction of nanozyme based with mixed valence manganese oxide loaded on defective metal-organic frameworks for sensitive detection of biomarker procalcitonin

Biosensors and Bioelectronics, Год журнала: 2025, Номер 278, С. 117339 - 117339

Опубликована: Март 11, 2025

Язык: Английский

---

Nanozymes in biomedicine: Unraveling trends, research foci, and future trajectories via bibliometric insights (from 2007 to 2024)

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142798 - 142798

Опубликована: Апрель 1, 2025

Язык: Английский

---