Cu2O/CuVO3 Nano‐Heterojunction as a Highly Active Therapeutic Catalyst for Aggravating Redox Dyshomeostasis of Neoplastic Cells DOI

Weisheng Zhu,

J. F. Wu, Yuejun Kang

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: June 1, 2025

Abstract Redox dyshomeostasis is both a hallmark and vulnerability of cancer cells, offering multiple avenues for therapeutic intervention. Herein, belt‐like nano‐heterojunction Cu 2 O/CuVO 3 (CVO) developed as potential redox inducer by exacerbating ROS levels compromising antioxidant defense without the need exogenous stimulations. Steady‐state analysis reveals that CVO exhibits extraordinary reaction velocity catalytic efficiency ( V max = 2.32 µ m s −1 , K cat 0.49 ) in production hydroxyl radicals (·OH). Likewise, density functional theory (DFT) calculations indicate superb charge‐transferring properties heterojunction structure unique surface coverage rate primarily dominate high‐efficient reactions. Noteworthy, capable inducing mitochondria dysfunction aggravating cellular imbalance, thereby triggering cell death pathways generating synergistic effects. Transcriptomics outcomes further demonstrate exerts significant distinct effects on key biological processes tumor encompassing but not limited to canonical such apoptosis, ferroptosis, cuprotosis. Both vitro vivo studies pronounced tumor‐inhibitory efficacy CVO, which paves promising pathway development novel nanocatalysts effectively target through amplification dyshomeostasis.

Language: Английский

Improving Nanozyme-Based Colorimetric Assays through Medium Composition Optimization in Nanozyme-Substrate Reaction DOI
Pavel Khramtsov, Р. Г. Валеев,

М. А. Еремина

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Nanozymes, while promising alternatives to natural peroxidases in colorimetric assays, are often hindered by lower catalytic efficiencies. Although numerous approaches have been developed improve signal intensity nanozyme-based optimization of the reaction medium which nanozyme interacts with substrate remains a significantly underexplored area. The vast majority studies rely on standard sodium acetate buffers or commercially sourced reagents optimized for horseradish peroxidase, neglecting unique properties different nanozymes. This work presents systematic 3,3',5,5'-tetramethylbenzidine (TMB)-based composition four common nanozymes: iron oxide, LaNiO3, Mn-doped CeO2, and platinum nanoparticles. Our findings reveal that buffer is suitable alternative enhance (41-68%) other Further ionic strength, organic cosolvent type concentration, TMB/H2O2 concentrations yielded improvements intensity, analytical sensitivity, assay time. study also identifies pitfalls encountered during conditions proposes potential solutions. We posit should be step development use undisclosed compositions avoided.

Language: Английский

Citations

2
Construction of nanozyme based with mixed valence manganese oxide loaded on defective metal-organic frameworks for sensitive detection of biomarker procalcitonin DOI

Suimin Deng,

Yun Hao, Lina Yang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117339 - 117339

Published: March 11, 2025

Language: Английский

Citations

1
Enhancing the Peroxidase-Mimicking Activity of Gold Nanoparticles for Lateral Flow Assays: Quantitative Evaluation in a Kinetic View DOI
Vasily G. Panferov,

Wenjun Zhang,

Nicholas D’Abruzzo

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Highly sensitive lateral flow immunoassays (LFIAs) are essential for various point-of-care applications, and gold nanoparticles (Au NPs) by far the most commonly used labels. However, conventional LFIAs often suffer from high detection limits (LOD) or low sensitivity. In this study, we investigated three strategies to enhance sensitivity of improving peroxidase-mimicking (POD) activity Au NPs. The POD unmodified NPs was negligible (<0.01 units/mg, U/mg). first strategy involved coupling with horseradish peroxidase (HRP), which increased 65 U/mg. second approach forming a thin palladium iridium shell on NPs, elevated 0.69-0.71 third binding mercury ions (Hg2+) resulting in up 3 Finally, developed simple quantitative model estimate LOD based kinetic parameters. Using Au-HRP conjugates, demonstrated that experimentally measured consistent calculated values. provides framework evaluating catalytic signal amplification can be guide development highly assays.

Language: Английский

Citations

0
Nanozymes in biomedicine: Unraveling trends, research foci, and future trajectories via bibliometric insights (from 2007 to 2024) DOI
Siyang Cao,

Yingchen Pang,

Yihao Wei

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142798 - 142798

Published: April 1, 2025

Language: Английский

Citations

0
Utilizing the Thermostability of Nanozymes for Joule Heating to Remove Background Peroxidase Activities in Lateral Flow Assays DOI
Vasily G. Panferov, Nikita A. Ivanov,

Wenjun Zhang

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Lateral flow assays (LFAs) are essential for point-of-care testing. The use of peroxidase-mimicking nanozymes as catalytic labels is an actively developing direction in LFA, primarily focused on enhancing sensitivity. However, endogenous peroxidases, naturally present various samples, can interfere with nanozyme signal amplification, leading to a high background and making visual detection more challenging. issue peroxidases particularly significant LFAs wash-free biosensors. In this study, we showcase the remarkable thermostability contrast enzymes, applied analytically relevant lateral aflatoxin B1. By employing Joule heating portable battery-powered device, test strips were rapidly heated 75-80 °C after completing conventional LFA process. This caused thermal denaturation without affecting Au@Pt nanozymes. As result, substrate oxidation strip was carried out solely by nanozymes, which reduced noise improved limit factor 3.5 compared assay heating.

Language: Английский

Citations

0
Nanozyme-based biosensors for food contaminants detection: advances, challenges, and prospects DOI
Zhilong Wang, Na Liu, Yingying Fan

et al.

Talanta, Journal Year: 2025, Volume and Issue: 295, P. 128290 - 128290

Published: May 11, 2025

Language: Английский

Citations

0
A Systematic Investigation of TMB Substrate Composition for Signal Enhancement in ELISA DOI
Pavel Khramtsov, Anastasia Novokshonova,

Zarina Galaeva

et al.

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: 704, P. 115908 - 115908

Published: May 23, 2025

Language: Английский

Citations

0
Cu2O/CuVO3 Nano‐Heterojunction as a Highly Active Therapeutic Catalyst for Aggravating Redox Dyshomeostasis of Neoplastic Cells DOI

Weisheng Zhu,

J. F. Wu, Yuejun Kang

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: June 1, 2025

Abstract Redox dyshomeostasis is both a hallmark and vulnerability of cancer cells, offering multiple avenues for therapeutic intervention. Herein, belt‐like nano‐heterojunction Cu 2 O/CuVO 3 (CVO) developed as potential redox inducer by exacerbating ROS levels compromising antioxidant defense without the need exogenous stimulations. Steady‐state analysis reveals that CVO exhibits extraordinary reaction velocity catalytic efficiency ( V max = 2.32 µ m s −1 , K cat 0.49 ) in production hydroxyl radicals (·OH). Likewise, density functional theory (DFT) calculations indicate superb charge‐transferring properties heterojunction structure unique surface coverage rate primarily dominate high‐efficient reactions. Noteworthy, capable inducing mitochondria dysfunction aggravating cellular imbalance, thereby triggering cell death pathways generating synergistic effects. Transcriptomics outcomes further demonstrate exerts significant distinct effects on key biological processes tumor encompassing but not limited to canonical such apoptosis, ferroptosis, cuprotosis. Both vitro vivo studies pronounced tumor‐inhibitory efficacy CVO, which paves promising pathway development novel nanocatalysts effectively target through amplification dyshomeostasis.

Language: Английский

Citations

0