Journal of Nanoparticle Research, Год журнала: 2025, Номер 27(4)
Опубликована: Март 20, 2025
Язык: Английский
Clinical and Translational Science, Год журнала: 2024, Номер 17(8)
Опубликована: Авг. 1, 2024
Abstract With advances in medical technology, extracellular vesicles, also known as exosomes, are gaining widespread attention because of their potential therapeutic applications. However, regulatory landscape is complex and varies across countries unique intracellular mechanisms action. The diversity manufacturing techniques renders standardization challenging, leading to a fragmented landscape. current global framework exosomes can be broadly classified into two strategies: one involves elucidating constituent components within the other examining physiological repercussions secretion. When using agents, they should governed similarly biological medicinal products. Similar biologics, have been analyzed determine particle size protein composition. An exosome‐based agent clinically approved after understanding its molecular composition structure demonstrating pharmacokinetics efficacy. efficacy challenging for agencies. This article reviews technical characteristics analyzes trends laws various countries, discusses chemistry, manufacturing, control requirements clinical
Язык: Английский
Процитировано
44
Bioactive Materials, Год журнала: 2024, Номер 36, С. 126 - 156
Опубликована: Март 2, 2024
Small extracellular vesicles (sEVs) are known to be secreted by a vast majority of cells. These sEVs, specifically exosomes, induce specific cell-to-cell interactions and can activate signaling pathways in recipient cells through fusion or interaction. nanovesicles possess several desirable properties, making them ideal for regenerative medicine nanomedicine applications. properties include exceptional stability, biocompatibility, wide biodistribution, minimal immunogenicity. However, the practical utilization particularly clinical settings at large scale, is hindered expensive procedures required their isolation, limited circulation lifetime, suboptimal targeting capacity. Despite these challenges, sEVs have demonstrated remarkable ability accommodate various cargoes found extensive applications biomedical sciences. To overcome limitations broaden potential applications, researchers should strive deepen understanding current loading, characterization techniques. Additionally, acquiring fundamental knowledge about origins employing state-of-the-art methodologies expand research scope. This review provides comprehensive overview exosome-based strategies diverse domains, encompassing cancer therapy, immunotherapy, biomarker Furthermore, we emphasize immense exosomes medicine.
Язык: Английский
Процитировано
35
Theranostics, Год журнала: 2024, Номер 14(5), С. 2099 - 2126
Опубликована: Янв. 1, 2024
Exosomes, which are small vesicles enclosed by a lipid bilayer and released many cell types, widely dispersed have garnered increased attention in the field of regenerative medicine due to their ability serve as indicators diseases agents with therapeutic potential. Exosomes play crucial role mediating intercellular communication through transfer biomolecules, including proteins, lipids, RNA, other molecular constituents, between cells. The targeted transport proteins nucleic acids specific cells has potential enhance or impair biological functions. applications, they can be used alone combination approaches. examination unique attributes functions these factors emerged prominent study realm biomedical research. This manuscript summarizes origins properties exosomes, structural, biological, physical, chemical aspects. paper offers complete recent progress tissue repair medicine, emphasizing possible implications methods forthcoming regeneration attempts.
Язык: Английский
Процитировано
25
Molecular Cancer, Год журнала: 2025, Номер 24(1)
Опубликована: Фев. 14, 2025
Extracellular vesicles (EVs), nanoscale secreted by cells, have attracted considerable attention in recent years due to their role tumor immunomodulation. These facilitate intercellular communication transporting proteins, nucleic acids, and other biologically active substances, they exhibit a dual development immune evasion mechanisms. Specifically, EVs can assist cells evading surveillance attack impairing cell function or modulating immunosuppressive pathways, thereby promoting progression metastasis. Conversely, also transport release immunomodulatory factors that stimulate the activation regulation of system, enhancing body's capacity combat malignant diseases. This functionality presents promising avenues targets for immunotherapy. By examining biological characteristics influence on immunity, novel therapeutic strategies be developed improve efficacy relevance cancer treatment. review delineates complex immunomodulation explores potential implications approaches, aiming establish theoretical foundation provide practical insights advancement future EVs-based immunotherapy strategies.
Язык: Английский
Процитировано
8
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Фев. 28, 2025
The extracellular matrix (ECM) is a complex network of biomolecules with varying pore sizes, posing challenge for the effective penetration lipid nanoparticles. In contrast, cell-derived nanoparticles, such as exosomes, have demonstrated ability to travel distant organs, indicating their capacity penetrate ECM. Here, we designed exosome-like vesicles (ELVs) inspired by exosomes' distinct transport phenomena. Specifically, integrated three exosomal components (anionic lipid, cholesterol, and aquaporin-1) associated into our ELVs mimic superior diffusion behavior exosomes over synthetic Surprisingly, both bulk- single-particle-diffusion studies revealed more than 33 times increase in coefficient within model ECM compared conventional Furthermore, show an 80% biological tissues. excellent was further validated vivo, where intratumoral injection showcased transport. These findings provide insights nanoparticle design improved tissue penetration.
Язык: Английский
Процитировано
3
ACS Applied Nano Materials, Год журнала: 2024, Номер 7(8), С. 8489 - 8506
Опубликована: Апрель 15, 2024
Cancer is the most challenging health complication worldwide. Scientific society has tried to decode its complications and developing a smart solution for mankind. Extracellular vesicles (EVs) are an emerging frontier in cancer research. EVs provide fresh insight into understanding of released from all active cells. classified some major classes, such as apoptotic bodies, microvesicles, exosomes. Exosomes among exciting tools current research they work cellular messengers (which reflects cell's healthy or pathological conditional status). In tumor microenvironment, regulate several complicated events uncontrolled cell growth, immune suppression, angiogenesis, extracellular matrix remodeling, metastasis, organ-specific drug therapeutic resistance, stem development. also source promising biomarkers involved both diagnosis prognosis performs role. Exosomes-based nanomedicine which application nanosize theranostics sensor orientation, supports overcoming management limitations, early therapeutics specific, effective, affordable. this review, we have highlighted integration exosomes nanotechnology cutting-edge approach precision oncology.
Язык: Английский
Процитировано
14
Biomaterials Science, Год журнала: 2024, Номер 12(14), С. 3500 - 3521
Опубликована: Янв. 1, 2024
This review systematically summarizes the cutting-edge methods for preparing engineered exosomes through cell engineering and exosome engineering, as well latest advancements of in therapeutic applications.
Язык: Английский
Процитировано
13
Bioengineering, Год журнала: 2025, Номер 12(1), С. 92 - 92
Опубликована: Янв. 20, 2025
Critical limb ischemia (CLI) poses a substantial and intricate challenge in vascular medicine, necessitating the development of innovative therapeutic strategies to address its multifaceted pathophysiology. Conventional revascularization approaches often fail adequately complexity CLI, identification alternative methodologies. This review explores uncharted territory beyond traditional therapies, focusing on potential two distinct yet interrelated entities: cell-derived extracellular vesicles (EVs) artificial nanovesicles. Cell-derived EVs are small membranous structures naturally released by cells, nanovesicles artificially engineered nanosized vesicles. Both these represent promising avenues for intervention. They act as carriers bioactive cargo, including proteins, nucleic acids, lipids, that can modulate cellular responses associated with ischemic tissue repair angiogenesis. also assesses evolving landscape CLI through unique perspective The spans spectrum from early preclinical investigations latest translational advancements, providing comprehensive overview current state research this emerging field. These groundbreaking vesicle therapies hold immense revolutionizing treatment paradigms.
Язык: Английский
Процитировано
2
Materials Today Bio, Год журнала: 2025, Номер 31, С. 101613 - 101613
Опубликована: Фев. 25, 2025
Язык: Английский
Процитировано
2
ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(10), С. 5960 - 5976
Опубликована: Июнь 28, 2024
Exosomes are nanoscale membrane bound vesicles secreted by almost all types of cells. Their unique attributes, such as minimal immunogenicity and compatibility with biological systems, make them novel carriers for drug delivery. These native exosomes harbor proteins, nucleic acids, small molecule compounds, fluorogenic agents. Moreover, through a combination chemical bioengineering methodologies, tailored to transport precise therapeutic payloads designated cells or tissues. In this review, we summarize the strategies exosome modification loading modalities in engineered exosomes. addition, provide an overview advances use targeted Lastly, discuss merits limitations chemically versus bioengineered exosome-mediated target therapies. insights offer additional options refining pharmaceutical development hold promise expediting successful translation from bench bedside.
Язык: Английский
Процитировано
7