Silver Nanoparticles Synthesized From Centella asiatica Extract and Asiatic Acid for Enhanced Biofilm Eradication of Streptococcus Associated With Oral Diseases
Scientifica,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
The
biofilm‐forming
ability
of
Streptococcus
species,
particularly
mutans
,
is
a
key
factor
in
the
pathogenesis
dental
caries
and
periodontitis.
Current
treatments
often
exhibit
limitations
such
as
incomplete
biofilm
eradication
cytotoxicity
to
host
tissues,
highlighting
need
for
innovative
biocompatible
therapeutic
approaches.
Therefore,
this
study
aimed
investigate
potential
Centella
asiatica
ethanolic
extract,
its
bioactive
triterpenoids
(asiatic
acid
madecassic
acid),
silver
nanoparticles
(AgNPs)
synthesized
from
extract
an
alternative
strategy
targeting
S.
biofilms.
antibacterial
antibiofilm
activities
AgNPs
against
species
were
evaluated,
alongside
assessments
on
human
gingival
fibroblast
(HGF‐1)
cells
using
MTT
assay.
exhibited
superior
antimicrobial
efficacy
compared
with
significantly
lower
minimum
inhibitory
concentration
(MIC)
bactericidal
(MBC)
values
(62.5/125
µg/mL)
ATCC
25175
mitis
49456,
their
potent
activity.
Moreover,
achieved
rapid
disruption,
reducing
biomass
by
76%
within
12
h
at
1/2
×
MIC,
outperforming
triterpenoids.
Scanning
electron
microscopy
further
revealed
substantial
extracellular
polymeric
substance
degradation
structural
disruption
upon
AgNP
treatment,
confirming
pronounced
efficacy.
In
addition,
demonstrated
favorable
biocompatibility,
maintaining
68%
cell
viability
cells,
suggesting
optimal
balance
between
potency
cytotoxicity.
synergistic
interaction
C.
phytochemicals
enhanced
nonfunctionalized
AgNPs.
These
findings
underscore
–based
novel,
plant‐derived
nanotechnological
approach
managing
oral
infections
caused
species.
This
not
only
contributes
development
strategies
but
also
paves
way
future
clinical
applications
healthcare.
Язык: Английский
Enhanced antibacterial and wound healing efficacy of a novel CQDs@AgNPs@CS-based nanocomposites: A multifunctional approach for advanced wound care
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 143621 - 143621
Опубликована: Апрель 1, 2025
Язык: Английский
Silver Nanoparticle-Induced Nephrotoxicity in Zebrafish (Danio rerio)
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(9), С. 4216 - 4216
Опубликована: Апрель 29, 2025
The
escalating
challenge
of
antibacterial
resistance
has
driven
the
widespread
use
silver
nanoparticles
(AgNPs)
due
to
their
potent
antimicrobial
properties.
AgNPs
can
be
synthesised
through
diverse
methods,
spanning
conventional
chemical
and
physical
routes
increasingly
favoured
biosynthesis
approach.
While
offering
environmental
advantages,
ecological
impact
biogenically
synthesized
AgNPs,
especially
on
aquatic
ecosystems,
requires
thorough
evaluation.
renal
system,
critical
for
maintaining
physiological
homeostasis
via
nephron-mediated
waste
removal,
fluid
regulation,
electrolyte
balance,
is
highly
vulnerable
toxicant-induced
damage,
which
negatively
affect
organismal
fitness.
This
study
aimed
assess
nephrotoxic
effects
using
entirely
“green”
zebrafish
after
96-h
exposures
three
distinct
concentrations
alongside
a
control
group.
Acridine
orange
fluorescence
microscopy
revealed
dose-dependent
histopathological
alterations
in
tissues.
Specifically,
at
0.031
μg/L
0.250
μg/L,
significant
changes
were
observed,
including
glomerular
shrinkage,
proliferation
hematopoietic
tissue,
dissociation
dilation
tubules,
melanomacrophage
aggregation.
At
5.000
prolonged
exposure
beyond
48
h
indicated
potential
tissue
cell
renewal,
suggesting
possible
compensatory
response.
These
results
demonstrate
sensitivity
kidneys
emphasize
imperative
comprehensive
vivo
toxicity
testing,
irrespective
synthesis
method,
accurately
evaluate
adverse
impacts
ensure
preservation
ecosystem
integrity.
Язык: Английский
New Strategies and Artificial Intelligence Methods for the Mitigation of Toxigenic Fungi and Mycotoxins in Foods
Toxins,
Год журнала:
2025,
Номер
17(5), С. 231 - 231
Опубликована: Май 7, 2025
The
proliferation
of
toxigenic
fungi
in
food
and
the
subsequent
production
mycotoxins
constitute
a
significant
concern
fields
public
health
consumer
protection.
This
review
highlights
recent
strategies
emerging
methods
aimed
at
preventing
fungal
growth
mycotoxin
contamination
matrices
as
opposed
to
traditional
approaches
such
chemical
fungicides,
which
may
leave
toxic
residues
pose
risks
human
animal
well
environment.
novel
methodologies
discussed
include
use
plant-derived
compounds
essential
oils,
classified
Generally
Recognized
Safe
(GRAS),
polyphenols,
lactic
acid
bacteria,
cold
plasma
technologies,
nanoparticles
(particularly
metal
silver
or
zinc
nanoparticles),
magnetic
materials,
ionizing
radiation.
Among
these,
bacteria
offer
eco-friendly
non-toxic
alternatives
conventional
fungicides
while
demonstrating
strong
antimicrobial
antifungal
properties;
oils
polyphenols
also
possess
antioxidant
activity.
Cold
radiation
enable
rapid,
non-thermal,
chemical-free
decontamination
processes.
Nanoparticles
materials
contribute
advantages
enhanced
stability,
controlled
release,
ease
separation.
Furthermore,
this
explores
advancements
application
artificial
intelligence,
particularly
machine
learning
methods,
for
identification
classification
species
predicting
products
culture
media.
Язык: Английский