Silver Nanoparticles Synthesized From Centella asiatica Extract and Asiatic Acid for Enhanced Biofilm Eradication of Streptococcus Associated With Oral Diseases

Scientifica, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

The biofilm‐forming ability of Streptococcus species, particularly mutans , is a key factor in the pathogenesis dental caries and periodontitis. Current treatments often exhibit limitations such as incomplete biofilm eradication cytotoxicity to host tissues, highlighting need for innovative biocompatible therapeutic approaches. Therefore, this study aimed investigate potential Centella asiatica ethanolic extract, its bioactive triterpenoids (asiatic acid madecassic acid), silver nanoparticles (AgNPs) synthesized from extract an alternative strategy targeting S. biofilms. antibacterial antibiofilm activities AgNPs against species were evaluated, alongside assessments on human gingival fibroblast (HGF‐1) cells using MTT assay. exhibited superior antimicrobial efficacy compared with significantly lower minimum inhibitory concentration (MIC) bactericidal (MBC) values (62.5/125 µg/mL) ATCC 25175 mitis 49456, their potent activity. Moreover, achieved rapid disruption, reducing biomass by 76% within 12 h at 1/2 × MIC, outperforming triterpenoids. Scanning electron microscopy further revealed substantial extracellular polymeric substance degradation structural disruption upon AgNP treatment, confirming pronounced efficacy. In addition, demonstrated favorable biocompatibility, maintaining 68% cell viability cells, suggesting optimal balance between potency cytotoxicity. synergistic interaction C. phytochemicals enhanced nonfunctionalized AgNPs. These findings underscore –based novel, plant‐derived nanotechnological approach managing oral infections caused species. This not only contributes development strategies but also paves way future clinical applications healthcare.

Language: Английский

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Enhanced antibacterial and wound healing efficacy of a novel CQDs@AgNPs@CS-based nanocomposites: A multifunctional approach for advanced wound care

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143621 - 143621

Published: April 28, 2025

Language: Английский

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Silver Nanoparticle-Induced Nephrotoxicity in Zebrafish (Danio rerio)

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4216 - 4216

Published: April 29, 2025

The escalating challenge of antibacterial resistance has driven the widespread use silver nanoparticles (AgNPs) due to their potent antimicrobial properties. AgNPs can be synthesised through diverse methods, spanning conventional chemical and physical routes increasingly favoured biosynthesis approach. While offering environmental advantages, ecological impact biogenically synthesized AgNPs, especially on aquatic ecosystems, requires thorough evaluation. renal system, critical for maintaining physiological homeostasis via nephron-mediated waste removal, fluid regulation, electrolyte balance, is highly vulnerable toxicant-induced damage, which negatively affect organismal fitness. This study aimed assess nephrotoxic effects using entirely "green" zebrafish after 96-h exposures three distinct concentrations alongside a control group. Acridine orange fluorescence microscopy revealed dose-dependent histopathological alterations in tissues. Specifically, at 0.031 μg/L 0.250 μg/L, significant changes were observed, including glomerular shrinkage, proliferation hematopoietic tissue, dissociation dilation tubules, melanomacrophage aggregation. At 5.000 prolonged exposure beyond 48 h indicated potential tissue cell renewal, suggesting possible compensatory response. These results demonstrate sensitivity kidneys emphasize imperative comprehensive vivo toxicity testing, irrespective synthesis method, accurately evaluate adverse impacts ensure preservation ecosystem integrity.

Language: Английский

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Silver Nanoparticles (AgNPs) as Potential Antiviral Agents: Synthesis, Biophysical Properties, Safety, Challenges and Future Directions─Update Review

Molecules, Journal Year: 2025, Volume and Issue: 30(9), P. 2004 - 2004

Published: April 30, 2025

AgNPs have gained significant attention due to their unique physicochemical properties, making them valuable across a range of fields including medicine, textiles, and household products. With strong antimicrobial antiviral shown promise in treating infections, particularly wound care management. This review explores the mechanisms underlying activities AgNPs, as well methods used for synthesis, which include physical, chemical, biological approaches. The also addresses potential limitations cytotoxicity humans environment. interaction between microorganisms, viruses, varies based on synthesis particle morphology. As viral resistant strains, present major global health challenges, there is growing need alternative therapies. Metal nanoparticles like offer advantages over conventional drugs broad target range, reduces likelihood resistance development. highlights AgNPs’ effectiveness against variety such HIV, hepatitis B, respiratory syncytial virus, discusses use novel treatments. examines toxicity, offering insights into future therapeutic roles.

Language: Английский

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New Strategies and Artificial Intelligence Methods for the Mitigation of Toxigenic Fungi and Mycotoxins in Foods

Toxins, Journal Year: 2025, Volume and Issue: 17(5), P. 231 - 231

Published: May 7, 2025

The proliferation of toxigenic fungi in food and the subsequent production mycotoxins constitute a significant concern fields public health consumer protection. This review highlights recent strategies emerging methods aimed at preventing fungal growth mycotoxin contamination matrices as opposed to traditional approaches such chemical fungicides, which may leave toxic residues pose risks human animal well environment. novel methodologies discussed include use plant-derived compounds essential oils, classified Generally Recognized Safe (GRAS), polyphenols, lactic acid bacteria, cold plasma technologies, nanoparticles (particularly metal silver or zinc nanoparticles), magnetic materials, ionizing radiation. Among these, bacteria offer eco-friendly non-toxic alternatives conventional fungicides while demonstrating strong antimicrobial antifungal properties; oils polyphenols also possess antioxidant activity. Cold radiation enable rapid, non-thermal, chemical-free decontamination processes. Nanoparticles materials contribute advantages enhanced stability, controlled release, ease separation. Furthermore, this explores advancements application artificial intelligence, particularly machine learning methods, for identification classification species predicting products culture media.

Language: Английский

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Transforming bone cancer treatment: a comprehensive review of green-synthesized metal nanoparticles

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 25, 2025

Osteosarcoma (OS), chondrosarcoma (CHS), and Ewing sarcoma (EWS) are the main types of bone cancer (BC). OS is most common BC in this group. It children older people, especially their long bones. Treatments for sarcomas tumors have slowly improved, so researchers began looking into additional alternative approaches to standard therapies. Therefore, ability precisely manipulate metallic nanoparticles (MNPs)' form, size, charge, surface modification makes them very useful treating cancer. However, due biocompatibility possible toxicity MNPs, MNP has limits clinical use BC. green synthesis MNPs achieved by bio-reducing ions, which results creation NPs, using living entities or extracts. Green derived from natural sources provide a secure environmentally responsible solution. Benefits include tailored medicine delivery biocompatibility. reduce damage healthy cells while improving targeting cells. In study, we reviewed how different synthesized methods can help treat various This work usual way making BC, problems with benefits future uses synthetic

Language: Английский

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Functionalized Silver Nanoparticles as Multifunctional Agents Against Gut Microbiota Imbalance and Inflammation

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(11), P. 815 - 815

Published: May 28, 2025

Human pathogenic fungi are the source of various illnesses, including invasive, cutaneous, and mucosal infections. One promising solution is using nanoparticles (NPs) as an antifungal agent. The current study aims to assess antimicrobial effects drug-loaded silver (AgNPs) with previously reported mebeverine analogue (MA) a potential drug candidate targeting gut microbiota inflammation in gastrointestinal tract. Density Functional Theory (DFT) calculations were conducted identify possible mechanisms by which AgNPs could prevent microorganisms from growing. In vitro ex vivo anti-inflammatory, antimicrobial, spasmolytic activities, hepatic cell morphology proliferation assessed. considered have activity against all tested fungal strains, Aspergillus niger, Penicillium chrysogenum, Fusarium moniliforme, yeasts, Candida albicans, Saccharomyces cerevisiae, good Gram-positive Gram-negative bacterial strains. results determination anti-inflammatory indicated that preserved MA’s decreased inflammation. A similar effect was observed measurements. Drug-loaded also influenced cells, indicating for improved liver therapeutic efficacy. Each test performed triplicate, mean values. Based on results, might be agent, maintaining Future preclinical experiments will contribute establishing properties AgNPs.

Language: Английский

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Fusarium Head Blight Management with Nanotechnology: Advances and Future Prospects

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102782 - 102782

Published: June 1, 2025

Language: Английский

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