Depending
on
the
measured
objects
and
specific
detection
requirements,
adjusting
shear
ratio
appropriately
is
crucial
for
optimizing
performance
of
quadriwave
lateral
shearing
interferometric
microscopy
(QWLSIM).
Hence,
in
QWLSIM
should
be
continuously
variable
practical
applications.
In
this
paper,
phase
using
interferometer
with
proposed.
We
use
a
globally
random
encoded
hybrid
grating
(GREHG),
which
comprises
an
amplitude
chessboard,
as
beam
splitter
interferometer.
The
coding
rule
GREHG
approximates
ideal
across
entire
based
optical
flux
constraint,
effectively
suppressing
all
higher-order
diffraction
orders
except
necessary
±1
pixel
light,
thus
achieving
ratio.
As
count
period
increases,
adjustment
approaches
condition
continuous
more
closely.
Simulation
experimental
results
demonstrate
that
approach
realizes
without
modifying
system,
great
significance
diverse
measurement
requirements.
Light Science & Applications,
Год журнала:
2024,
Номер
13(1)
Опубликована: Окт. 11, 2024
Abstract
Quantitative
phase
microscopies
(QPMs)
play
a
pivotal
role
in
bio-imaging,
offering
unique
insights
that
complement
fluorescence
imaging.
They
provide
essential
data
on
mass
distribution
and
transport,
inaccessible
to
techniques.
Additionally,
QPMs
are
label-free,
eliminating
concerns
of
photobleaching
phototoxicity.
However,
navigating
through
the
array
available
QPM
techniques
can
be
complex,
making
it
challenging
select
most
suitable
one
for
particular
application.
This
tutorial
review
presents
thorough
comparison
main
techniques,
focusing
their
accuracy
terms
measurement
precision
trueness.
We
focus
8
namely
digital
holographic
microscopy
(DHM),
cross-grating
wavefront
(CGM),
which
is
based
QLSI
(quadriwave
lateral
shearing
interferometry),
diffraction
(DPM),
differential
phase-contrast
(DPC)
microscopy,
phase-shifting
interferometry
(PSI)
imaging,
Fourier
(FPM),
spatial
light
interference
(SLIM),
transport-of-intensity
equation
(TIE)
For
this
purpose,
we
used
home-made
numerical
toolbox
discrete
dipole
approximation
(IF-DDA).
designed
compute
electromagnetic
field
at
sample
plane
microscope,
irrespective
object’s
complexity
or
illumination
conditions.
upgraded
enable
model
any
type
QPM,
take
into
account
shot
noise.
In
nutshell,
results
show
DHM
PSI
inherently
free
from
artefacts
rather
suffer
coherent
noise;
CGM,
DPC,
DPM
TIE,
there
trade-off
between
trueness,
balanced
by
varying
experimental
parameter;
FPM
SLIM
inherent
cannot
discarded
experimentally
cases,
not
quantitative
especially
large
objects
covering
part
view,
such
as
eukaryotic
cells.
ACS Sensors,
Год журнала:
2024,
Номер
9(3), С. 1049 - 1064
Опубликована: Март 14, 2024
The
development
of
efficient
nanoscale
photon
absorbers,
such
as
plasmonic
or
high-index
dielectric
nanostructures,
allows
the
remotely
controlled
release
heat
on
using
light.
These
photothermal
nanomaterials
have
found
applications
in
various
research
and
technological
fields,
ranging
from
materials
science
to
biology.
However,
measuring
thermal
fields
remains
an
open
challenge,
hindering
full
comprehension
control
phenomena.
Here,
we
review
discuss
existent
thermometries
suitable
for
single
nanoparticles
heated
under
illumination.
methods
are
classified
four
categories
according
region
where
they
assess
temperature:
(1)
average
temperature
within
a
diffraction-limited
volume,
(2)
at
immediate
vicinity
nanoparticle
surface,
(3)
itself,
(4)
map
around
with
spatial
resolution.
In
latter,
because
it
is
most
challenging
informative
type
method,
also
envisage
new
combinations
technologies
that
could
be
helpful
retrieving
maps.
Finally,
analyze
provide
examples
strategies
validate
results
obtained
different
thermometry
methods.
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 15, 2025
Optical
phase
imaging
has
become
a
pivotal
tool
in
biomedical
research,
enabling
label-free
visualization
of
transparent
specimens.
Traditional
optical
techniques,
such
as
Zernike
contrast
and
differential
interference
microscopy,
fall
short
providing
quantitative
information.
Digital
holographic
microscopy
(DHM)
addresses
this
limitation
by
offering
precise
measurements;
however,
off-axis
configurations,
particularly
Mach-Zehnder
Michelson-based
setups,
are
often
hindered
environmental
susceptibility
bulky
components
due
to
their
separate
reference
object
beam
paths.
In
work,
we
have
developed
meta-based
interferometric
system
using
common-path
DHM
configuration.
A
meta-biprism,
featuring
two
opposite
gradient
phases
created
GaN
nanopillars
selected
for
low
loss
durability,
serves
compact
efficient
splitter.
Our
effectively
captures
the
complex
wavefronts
samples,
retrieval
information,
which
demonstrate
standard
resolution
targets
human
lung
cell
lines.
Additionally,
our
exhibits
enhanced
temporal
stability
compared
conventional
reducing
fluctuations
over
extended
measurement
periods.
These
results
not
only
underline
potential
metasurfaces
advancing
capabilities
but
also
promise
significant
advancements
diagnostics.
International Journal of Applied Glass Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 14, 2025
Abstract
Ultrafast
laser
irradiation
of
glass
enables
highly
localized
structural
transformations
within
the
material's
bulk,
unlocking
diverse
applications
in
photonics,
data
storage,
and
microfabrication.
Here,
we
provide
a
concise
yet
comprehensive
overview
main
types
femtosecond
laser‐induced
modifications
silica‐based
glasses
(Types
I,
II,
III,
X,
related
crystalline
transformations),
highlighting
their
distinct
features
underlying
thermal‐
pressure‐driven
mechanisms.
This
review
offers
current
state‐of‐the‐art
perspective
on
various
modifications,
while
also
presenting
new
nanoscale
insights
through
advanced
scattering
scanning
near‐field
optical
microscopy
nano‐Fourier
transform
infrared
spectroscopy,
discussing
densification
mechanisms
behind.
Finally,
outline
broader
perspectives,
from
fundamental
research
directions
to
industry
developments,
inspire
future
advances
next‐generation
technologies.
Label-free
thermometry
is
a
pivotal
tool
for
many
disciplines.
However,
most
current
approaches
are
only
suitable
planar
heat
sources
in
steady
state,
thereby
restricting
the
range
of
systems
that
can
be
reliably
studied.
Here,
we
introduce
pump
probe-based
optical
diffraction
tomography
(ODT)
as
method
to
map
temperature
precisely
and
accurately
three
dimensions
(3D)
at
single-particle
level.
To
do
so,
first
systematically
characterize
thermal
landscape
model
system
consisting
gold
nanorods
microchamber
then
benchmark
results
against
simulations
quantitative
phase
imaging
thermometry.
We
apply
ODT
resolve
landscapes
inaccessible
other
label-free
form
nonplanar
embedded
complex
environments
freely
diffusing
microchamber.
Last,
foresee
our
approach
will
find
applications
where
routine
characterization
heterogeneous
nanoparticles
samples
3D
or
non-steady
state
required.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Апрель 12, 2024
The
continuous
balance
of
growth
and
degradation
inside
cells
maintains
homeostasis.
Disturbance
this
by
internal
or
external
factors
cause
state
disease,
while
effective
disease
treatments
seek
to
restore
balance.
Here,
we
present
a
method
based
on
quantitative
phase
imaging
(QPI)
measurements
cell
mass
the
velocity
transport
quantify
within
intracellular
control
volumes.
result,
which
call
Lagrangian
velocimetry
for
net
(LVING),
provides
high
resolution
maps
biomass
production
degradation.
We
use
LVING
in
different
regions
during
phases
cycle.
can
also
be
used
quantitatively
compare
effect
range
chemotherapy
drug
doses
subcellular
processes.
Finally,
applied
characterize
autophagy
machinery
cells.
Overall,
reveals
both
structure
distribution
basal
cells,
as
well
disruptions
that
occur
alterations
state.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Янв. 25, 2024
The
article
introduces
an
optical
microscopy
technique
capable
of
simultaneously
acquiring
quantitative
fluorescence
and
phase
(or
equivalently
wavefront)
images
with
a
single
camera
sensor,
avoiding
any
delay
between
both
images,
or
registration
acquired
separately.
method
is
based
on
the
use
2-dimensional
diffraction
grating
(aka
cross-grating)
positioned
at
millimeter
distance
from
2-color
camera.
Fluorescence
wavefront
are
extracted
two
color
channels
camera,
retrieved
by
image
demodulation.
applicability
illustrated
various
samples,
namely
fluorescent
micro-beads,
bacteria
mammalian
cells.
ACS Nano,
Год журнала:
2024,
Номер
18(29), С. 19247 - 19256
Опубликована: Июль 9, 2024
Quantitative
phase
imaging
enables
precise
and
label-free
characterizations
of
individual
nano-objects
within
a
large
volume,
without
priori
knowledge
the
sample
or
system.While
emerging
common
path
implementations
are
simple
enough
to
promise
broad
dissemination,
their
sensitivity
still
falls
short
precisely
estimating
mass
polarizability
vesicles,
viruses,
nanoparticles
in
single-shot
acquisitions.In
this
paper,
we
revisit
Zernike
filtering
concept,
originally
crafted
for
intensity-only
detectors,
with
aim
adapting
it
wavefront
imaging.We
demonstrate,
through
numerical
simulation
experiments
based
on
high-resolution
sensing,
that
Fourier-plane
add-on
can
significantly
enhance
subdiffraction
objects�achieving
over
an
order
magnitude
increase
(×12)�while
allowing
quantitative
retrieval
both
intensity
phase.This
advancement
allows
more
nano-object
detection
metrology.
