Phase microscopy using the quadriwave lateral shearing interferometer with continuously variable shear ratio DOI
Hui Zhong, Yanqiu Li, Ke Liu

et al.

Published: Dec. 13, 2024

Depending on the measured objects and specific detection requirements, adjusting shear ratio appropriately is crucial for optimizing performance of quadriwave lateral shearing interferometric microscopy (QWLSIM). Hence, in QWLSIM should be continuously variable practical applications. In this paper, phase using interferometer with proposed. We use a globally random encoded hybrid grating (GREHG), which comprises an amplitude chessboard, as beam splitter interferometer. The coding rule GREHG approximates ideal across entire based optical flux constraint, effectively suppressing all higher-order diffraction orders except necessary ±1 pixel light, thus achieving ratio. As count period increases, adjustment approaches condition continuous more closely. Simulation experimental results demonstrate that approach realizes without modifying system, great significance diverse measurement requirements.

Language: Английский

Quantitative phase microscopies: accuracy comparison DOI Creative Commons
Patrick C. Chaumet, Pierre Bon,

Guillaume Maire

et al.

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Oct. 11, 2024

Abstract Quantitative phase microscopies (QPMs) play a pivotal role in bio-imaging, offering unique insights that complement fluorescence imaging. They provide essential data on mass distribution and transport, inaccessible to techniques. Additionally, QPMs are label-free, eliminating concerns of photobleaching phototoxicity. However, navigating through the array available QPM techniques can be complex, making it challenging select most suitable one for particular application. This tutorial review presents thorough comparison main techniques, focusing their accuracy terms measurement precision trueness. We focus 8 namely digital holographic microscopy (DHM), cross-grating wavefront (CGM), which is based QLSI (quadriwave lateral shearing interferometry), diffraction (DPM), differential phase-contrast (DPC) microscopy, phase-shifting interferometry (PSI) imaging, Fourier (FPM), spatial light interference (SLIM), transport-of-intensity equation (TIE) For this purpose, we used home-made numerical toolbox discrete dipole approximation (IF-DDA). designed compute electromagnetic field at sample plane microscope, irrespective object’s complexity or illumination conditions. upgraded enable model any type QPM, take into account shot noise. In nutshell, results show DHM PSI inherently free from artefacts rather suffer coherent noise; CGM, DPC, DPM TIE, there trade-off between trueness, balanced by varying experimental parameter; FPM SLIM inherent cannot discarded experimentally cases, not quantitative especially large objects covering part view, such as eukaryotic cells.

Language: Английский

Citations

10
Thermometries for Single Nanoparticles Heated with Light DOI
Luciana P. Martinez,

Maria Villarreal,

Cecilia Zaza

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(3), P. 1049 - 1064

Published: March 14, 2024

The development of efficient nanoscale photon absorbers, such as plasmonic or high-index dielectric nanostructures, allows the remotely controlled release heat on using light. These photothermal nanomaterials have found applications in various research and technological fields, ranging from materials science to biology. However, measuring thermal fields remains an open challenge, hindering full comprehension control phenomena. Here, we review discuss existent thermometries suitable for single nanoparticles heated under illumination. methods are classified four categories according region where they assess temperature: (1) average temperature within a diffraction-limited volume, (2) at immediate vicinity nanoparticle surface, (3) itself, (4) map around with spatial resolution. In latter, because it is most challenging informative type method, also envisage new combinations technologies that could be helpful retrieving maps. Finally, analyze provide examples strategies validate results obtained different thermometry methods.

Language: Английский

Citations

7
Quantitative Phase Imaging with a Meta-Based Interferometric System DOI Creative Commons
Cheng Hung Chu,

Chen-Ming Tsai,

Takeshi Yamaguchi

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Optical phase imaging has become a pivotal tool in biomedical research, enabling label-free visualization of transparent specimens. Traditional optical techniques, such as Zernike contrast and differential interference microscopy, fall short providing quantitative information. Digital holographic microscopy (DHM) addresses this limitation by offering precise measurements; however, off-axis configurations, particularly Mach-Zehnder Michelson-based setups, are often hindered environmental susceptibility bulky components due to their separate reference object beam paths. In work, we have developed meta-based interferometric system using common-path DHM configuration. A meta-biprism, featuring two opposite gradient phases created GaN nanopillars selected for low loss durability, serves compact efficient splitter. Our effectively captures the complex wavefronts samples, retrieval information, which demonstrate standard resolution targets human lung cell lines. Additionally, our exhibits enhanced temporal stability compared conventional reducing fluctuations over extended measurement periods. These results not only underline potential metasurfaces advancing capabilities but also promise significant advancements diagnostics.

Language: Английский

Citations

0
LVING reveals the intracellular structure of cell growth DOI Creative Commons
Soorya Pradeep, Thomas A. Zangle

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 12, 2024

The continuous balance of growth and degradation inside cells maintains homeostasis. Disturbance this by internal or external factors cause state disease, while effective disease treatments seek to restore balance. Here, we present a method based on quantitative phase imaging (QPI) measurements cell mass the velocity transport quantify within intracellular control volumes. result, which call Lagrangian velocimetry for net (LVING), provides high resolution maps biomass production degradation. We use LVING in different regions during phases cycle. can also be used quantitatively compare effect range chemotherapy drug doses subcellular processes. Finally, applied characterize autophagy machinery cells. Overall, reveals both structure distribution basal cells, as well disruptions that occur alterations state.

Language: Английский

Citations

3
Non-contact lensless holographic reconstruction of diffractive intraocular lenses profiles DOI Creative Commons
Rosa Vila-Andrés, Anabel Martínez-Espert, Walter D. Furlan

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 2, 2025

Language: Английский

Citations

0
Non–steady state thermometry with optical diffraction tomography DOI Creative Commons
Adarsh B. Vasista, Bernard Ciraulo, Falko Schmidt

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(12)

Published: March 22, 2024

Label-free thermometry is a pivotal tool for many disciplines. However, most current approaches are only suitable planar heat sources in steady state, thereby restricting the range of systems that can be reliably studied. Here, we introduce pump probe-based optical diffraction tomography (ODT) as method to map temperature precisely and accurately three dimensions (3D) at single-particle level. To do so, first systematically characterize thermal landscape model system consisting gold nanorods microchamber then benchmark results against simulations quantitative phase imaging thermometry. We apply ODT resolve landscapes inaccessible other label-free form nonplanar embedded complex environments freely diffusing microchamber. Last, foresee our approach will find applications where routine characterization heterogeneous nanoparticles samples 3D or non-steady state required.

Language: Английский

Citations

3
Single-shot quantitative phase-fluorescence imaging using cross-grating wavefront microscopy DOI Creative Commons

Baptiste Marthy,

Maëlle Bénéfice,

Guillaume Baffou

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 25, 2024

The article introduces an optical microscopy technique capable of simultaneously acquiring quantitative fluorescence and phase (or equivalently wavefront) images with a single camera sensor, avoiding any delay between both images, or registration acquired separately. method is based on the use 2-dimensional diffraction grating (aka cross-grating) positioned at millimeter distance from 2-color camera. Fluorescence wavefront are extracted two color channels camera, retrieved by image demodulation. applicability illustrated various samples, namely fluorescent micro-beads, bacteria mammalian cells.

Language: Английский

Citations

2
Optical diffraction tomography based on quadriwave lateral shearing interferometry DOI
Xun Yuan, Junwei Min, Yuan Zhou

et al.

Optics & Laser Technology, Journal Year: 2024, Volume and Issue: 177, P. 111124 - 111124

Published: May 4, 2024

Language: Английский

Citations

2
Enhanced Quantitative Wavefront Imaging for Nano-Object Characterization DOI Creative Commons
Clémence Gentner, Benoît Rogez, Hadrien M. L. Robert

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(29), P. 19247 - 19256

Published: July 9, 2024

Quantitative phase imaging enables precise and label-free characterizations of individual nano-objects within a large volume, without priori knowledge the sample or system.While emerging common path implementations are simple enough to promise broad dissemination, their sensitivity still falls short precisely estimating mass polarizability vesicles, viruses, nanoparticles in single-shot acquisitions.In this paper, we revisit Zernike filtering concept, originally crafted for intensity-only detectors, with aim adapting it wavefront imaging.We demonstrate, through numerical simulation experiments based on high-resolution sensing, that Fourier-plane add-on can significantly enhance subdiffraction objects�achieving over an order magnitude increase (×12)�while allowing quantitative retrieval both intensity phase.This advancement allows more nano-object detection metrology.

Language: Английский

Citations

2
Temperature Sensing in Space and Transparent Media: Advancements in Off-Axis Digital Holography and the Temperature Coefficient of Refractive Index DOI Creative Commons
Davood Khodadad, Behnam Tayebi, Amin Saremi

et al.

Applied Sciences, Journal Year: 2023, Volume and Issue: 13(14), P. 8423 - 8423

Published: July 21, 2023

An off-axis digital holographic interferometry technique integrated with a Mach–Zehnder interferometer based setup is demonstrated for measuring the temperature and profile of transparent medium. This offers several advantages: it does not require precise optomechanical adjustments or accurate definition frequency carrier mask, making simple cost-effective. Additionally, high-quality optics are necessary. The methodology relies on phase difference between two digitally reconstructed complex wave fields utilizing coefficient refractive index. In this way, we presented an equation as function changes approach simplifies calculation process avoids burden complicated mathematical inversions, such inverse Abel transformation. It also eliminates need additional work Lorentz–Lorentz Gladstone–Dale relation can be extend 3D measurements.

Language: Английский

Citations

5