The
acute
sensory
behaviour
in
living
organisms
relies
on
the
highly
efficient
transport
of
ions
biological
nanochannels,
which
has
inspired
design
and
applications
artificial
solid-state
nanochannels
field
sensitive
analysis.
application
for
analysis
is
now
widely
investigated,
a
variety
sensors
have
been
developed.
By
coupling
reliable
nanochannel
fabrication
techniques
with
multitude
surface
modification
strategies,
novel
customized
sensing
capabilities
are
generated
by
integration
recognition
elements
nanochannels.
altered
physicochemical
properties
these
will
be
manifested
steady-state
currents
when
they
affected
target
analyte.
In
this
mini-review,
we
focus
emerging
based
different
processes
such
as
electron-beam
etching,
anodic
oxidation,
ion
track
self-assembly.
Also,
modifications
discussed,
including
nucleic
acids,
proteins,
small
molecules,
responsive
materials.
key
factors
during
detection
also
reviewed,
charge,
channel
size,
wettability.
bioinspired
exploration
molecules
(gas
drug
molecules)
concisely
presented.
Furthermore,
discuss
future
developments
challenges.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(19), С. 7470 - 7478
Опубликована: Май 2, 2024
MicroRNAs
(miRNAs)
are
endogenous
and
noncoding
single-stranded
RNA
molecules
with
a
length
of
approximately
18–25
nucleotides,
which
play
an
undeniable
role
in
early
cancer
screening.
Therefore,
it
is
very
important
to
develop
ultrasensitive
highly
specific
method
for
detecting
miRNAs.
Here,
we
present
bottom-up
assembly
approach
modifying
glass
microtubes
silica
nanowires
(SiNWs)
label-free
sensing
platform
miRNA-21
detection.
The
three-dimensional
(3D)
networks
formed
by
SiNWs
make
them
abundant
accessible
sites
binding
peptide
nucleic
acid
(PNA).
As
receptor,
PNA
has
no
phosphate
groups
exhibits
overall
electrically
neutral
state,
resulting
relatively
small
repulsion
between
RNA,
can
improve
the
hybridization
efficiency.
SiNWs-filled
microtube
(SiNWs@GMT)
sensor
enables
ultrasensitive,
detection
limit
as
low
1
aM
at
range
aM–100
nM.
Noteworthy,
still
detect
102–108
fM
complex
solutions
containing
1000-fold
homologous
interference
high
anti-interference
performance
specifically
recognize
target
presence
other
miRNAs
distinguish
1-,
3-mismatch
nucleotide
sequences.
Significantly,
able
lysate
breast
cell
lines
(e.g.,
MCF-7
cells
MDA-MB-231
cells),
indicating
that
good
potential
screening
cancers.
Human
sweat
has
the
potential
to
be
sufficiently
utilized
for
noninvasive
monitoring.
Given
complexity
of
secretion,
sensitivity
and
selectivity
monitoring
should
further
improved.
Here,
we
developed
an
olfactory-inspired
separation-sensing
nanochannel-based
electronic
sensitive
selective
monitoring,
which
was
simultaneously
endowed
with
interferent
separation
target
detection
performances.
The
special
strategy
imparts
functional
composite
membranes
a
high
113
mV
dec–1
potassium
detection.
excellent
mechanical
properties
conformability
Kevlar
aramid
nanofiber
layer
bring
well-wearing
performances
realize
continuous
wireless
recognition
between
molecules
ions
is
proved
at
molecular
level
in
detail
article.
replacement
proves
universality
performance
enhancement
intricate
biofluid
analysis
systems.
Accounts of Chemical Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 28, 2025
ConspectusIons
are
the
crucial
signaling
components
for
living
organisms.
In
cells,
their
transportation
across
pore-forming
membrane
proteins
is
vital
regulating
physiological
functions,
such
as
generating
ionic
current
signals
in
response
to
target
molecule
recognition.
This
ion
transport
affected
by
confined
interactions
and
local
environments
within
protein
pore.
Therefore,
can
efficiently
transduce
characteristics
of
each
into
ion-transport-mediated
with
high
sensitivity.
Inspired
nature,
various
pores
have
been
developed
high-throughput
label-free
nanopore
sensors
single-molecule
detection,
enabling
rapid
accurate
readouts.
particular,
aerolysin,
a
key
virulence
factor
Aeromonas
hydrophila,
exhibits
sensitivity
fingerprints
detecting
subtle
differences
sequence,
conformation,
structure
DNA,
proteins,
polypeptides,
oligosaccharides,
other
molecules.
Aerolysin
features
cap
that
approximately
14
nm
wide
on
cis
side
central
pore
about
10
long
minimum
diameter
around
1
nm.
Its
lumen,
11
charged
rings
at
two
entrances
neutral
amino
acids
between,
facilitates
dwelling
single
analyte
characteristic
enables
rich
between
well-defined
residues
analyte.
As
result,
signal
offers
unique
molecular
fingerprint,
extending
beyond
traditional
volume
exclusion
model
sensing.
2006,
aerolysin
was
first
reported
discriminate
conformational
peptides,
opening
door
rapidly
growing
field
electrochemistry.
Over
years,
mutant
nanopores
emerged,
associated
advanced
instrumentation
data
analysis
algorithms,
simultaneous
identification
over
30
targets
number
still
increasing.
electrochemistry
particular
allows
time-resolved
qualitative
quantitative
ranging
from
DNA
sequencing,
proteomics,
enzyme
kinetics,
reactions
potential
clinical
diagnostics.
Especially,
feasibility
dynamic
would
revolutionize
omics
studies
level,
paving
way
promising
temporal
omics.
Despite
success
this
approach
so
far,
it
remains
challenging
understand
how
correlate
distinguishable
signatures.
Recent
attempts
added
correction
terms
account
variations
mobility
caused
Account,
we
revisit
origin
blockade
induced
molecules
inside
nanopore.
We
highlight
contributions
noncovalent
sensing
ability
through
corrected
conductance
model.
Account
then
describes
design
interaction
networks
nanopore,
including
electrostatic,
hydrophobic,
hydrogen-bonding,
cation−π,
ion–charged
acid
interactions,
ultrasensitive
biomolecular
quantification.
Finally,
provide
an
outlook
further
understanding
network
improving
manipulating
fine-tuning
toward
broad
range
practical
applications.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 3, 2024
Constructing
stable,
portable
sensors
and
revealing
their
mechanisms
is
challenging.
Ion
metal-organic
frameworks
(IMOFs)
are
poised
to
serve
as
highly
effective
electrochemical
for
detecting
organophosphorus
pesticides
(OPs),
leveraging
unique
charge
properties.
In
this
work,
an
amino-modified
IMOF
was
constructed
combined
with
near-field
communication
(NFC)
technology
develop
a
portable,
touchless,
battery-free
biosensor
Analytical Chemistry,
Год журнала:
2024,
Номер
96(41), С. 16415 - 16424
Опубликована: Окт. 3, 2024
The
detection
of
DNA
methyltransferase
(MTase)
was
crucial
for
understanding
gene
expression
regulation,
cancer
mechanisms,
and
various
biological
processes,
contributing
significantly
to
disease
diagnosis
drug
development.
Herein,
a
nanopore
sensor
based
on
cascaded
signal
amplification
walker
autocatalytic
hybridization
reaction
(AHR)
developed
the
ultrasensitive
determination
MTases.
In
presence
Dam
MTase,
hairpin
structure
H
