A general method for the development of multicolor biosensors with large dynamic ranges

Nature Chemical Biology, Год журнала: 2023, Номер 19(9), С. 1147 - 1157

Опубликована: Июнь 8, 2023

Fluorescent biosensors enable the study of cell physiology with spatiotemporal resolution; yet, most suffer from relatively low dynamic ranges. Here, we introduce a family designed Förster resonance energy transfer (FRET) pairs near-quantitative FRET efficiencies based on reversible interaction fluorescent proteins fluorescently labeled HaloTag. These enabled straightforward design for calcium, ATP and NAD+ unprecedented The color each these can be readily tuned by changing either protein or synthetic fluorophore, which enables simultaneous monitoring free in different subcellular compartments following genotoxic stress. Minimal modifications furthermore allow their readout to switched fluorescence intensity, lifetime bioluminescence. thus establish new concept development highly sensitive tunable biosensors.

Язык: Английский

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Recent advances in FRET-Based biosensors for biomedical applications

Analytical Biochemistry, Год журнала: 2021, Номер 630, С. 114323 - 114323

Опубликована: Июль 31, 2021

Язык: Английский

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Advances in FRET‐based biosensors from donor‐acceptor design to applications

Aggregate, Год журнала: 2023, Номер 5(2)

Опубликована: Ноя. 12, 2023

Abstract Fluorogenic biosensors are essential tools widely used in biomedicine, chemical biology, environmental protection and food safety. Fluorescence resonance energy transfer (FRET) is a crucial technique for developing fluorogenic that provide mechanistic insight into bioprocesses through time‐spatial bioimaging living cells organisms. Although extensive FRET‐based sensors have been developed detecting or imaging analytes of interest over the past decade, few comprehensive reviews summarized recent studies from fundamental angle about design application. In this work, advance discovery FRET using donor‐acceptor dye combinations described they classified based on different types analytes, such as mall molecules, proteins, enzymes, nucleic acids metal ions. This review provides molecular‐level inspiration biosensors, aiding their application biosensing bioimaging.

Язык: Английский

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Single‐Walled Carbon Nanotube‐Based Optical Nano/Biosensors for Biomedical Applications: Role in Bioimaging, Disease Diagnosis, and Biomarkers Detection

Advanced Materials Technologies, Год журнала: 2024, Номер 9(20)

Опубликована: Май 16, 2024

Abstract The convergence of advanced nanotechnology with disease diagnosis has ushered in a transformative era healthcare, empowering early and accurate detection diseases paving the way for timely interventions, improved treatment outcomes, enhanced patient well‐being. development novel materials is frequently impetus behind significant advancements sensor technology. Among them, single‐walled carbon nanotubes (SWCNTSs) have emerged as promising nanomaterials developing biosensors. Their unique optical, electrical, biocompatibility properties make them candidates enhancing sensitivity real‐time monitoring capabilities biosensors, well enabling various bioimaging techniques. Recent studies demonstrated utility SWCNTS‐based biosensors biological analytes, such nitric oxide hydrogen peroxide (H 2 O ), potential implications understanding therapeutic response assessment. Moreover, SWCNTSs shown promise applications, including fluorescence, Raman spectroscopy, photoluminescence imaging samples. This article delves into core principles, design strategies, operational mechanisms that underpin SWCNTS‐bioimaging techniques‐based It emphasizes on their versatile functionalization nanotubes, laying foundation integration biosensor platforms applications aimed at diagnosing wide spectrum infectious diseases, cancer, neurological disorders, metabolic conditions.

Язык: Английский

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Multiphoton intravital microscopy of rodents

Nature Reviews Methods Primers, Год журнала: 2022, Номер 2(1)

Опубликована: Ноя. 10, 2022

Язык: Английский

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Advanced anticounterfeiting polymer inks for high-level encryption and authentication technologies

Progress in Materials Science, Год журнала: 2025, Номер unknown, С. 101487 - 101487

Опубликована: Апрель 1, 2025

Язык: Английский

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Single-cell dynamics of pannexin-1-facilitated programmed ATP loss during apoptosis

eLife, Год журнала: 2020, Номер 9

Опубликована: Окт. 14, 2020

ATP is essential for all living cells. However, how dead cells lose has not been well investigated. In this study, we developed new FRET biosensors dual imaging of intracellular level and caspase-3 activity in single apoptotic cultured human We show that the cytosolic starts to decrease immediately after activation caspase-3, process completed typically within 2 hr. The was facilitated by caspase-dependent cleavage plasma membrane channel pannexin-1, indicating cell a 'programmed' process. Apoptotic deficient pannexin-1 sustained ability produce through glycolysis consume ATP, did stop wasting glucose much longer period than normal Thus, plays role arresting metabolic cells, most likely facilitating loss ATP.

Язык: Английский

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Genetically encoded sensors towards imaging cAMP and PKA activity in vivo

Journal of Neuroscience Methods, Год журнала: 2021, Номер 362, С. 109298 - 109298

Опубликована: Июль 31, 2021

Язык: Английский

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Principles and applications of green fluorescent protein-based biosensors: a mini-review

The Analyst, Год журнала: 2023, Номер 148(13), С. 2882 - 2891

Опубликована: Янв. 1, 2023

An overview of the principles and applications GFP-based biosensors.

Язык: Английский

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Two-Dimensional Metalloporphyrinic Framework Nanosheet-Based Dual-Mechanism-Driven Ratiometric Electrochemiluminescent Biosensing of Protein Kinase Activity

ACS Applied Bio Materials, Год журнала: 2021, Номер 4(2), С. 1616 - 1623

Опубликована: Янв. 26, 2021

A dual-mechanism-driven ratiometric electrochemiluminescent (ECL) biosensor was developed for the ultrasensitive detection of protein kinase activity, which based on a competitive catalytic reaction and resonance energy transfer (RET) by assembling gold nanoparticles (GNPs) two-dimensional (2D) porphyrinic metal–organic framework (MOF) nanosheets. In this work, an ECL competing dissolved O2 proceeded between 2D copper-based zinc MOF (Cu–TCPP(Zn)) nanosheets luminol. Meanwhile, cathodic singlet oxygen (1O2), derived from electrocatalytic Cu–TCPP(Zn), would be reduced assembled GNPs due to RET, while anodic emission luminol could enhanced with excellent activity. With (PKA) as example, exhibited broad linear range (0.005–5.0 U mL–1) sensitive limit (0.0037 mL–1). Compared traditional single-mechanism-driven sensing strategies, may provide effective method design green sensors.

Язык: Английский

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