Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Ноя. 7, 2022
Abstract
Microbial
consortia
have
been
considered
potential
platforms
for
bioprocessing
applications.
However,
the
complexity
in
process
control
owing
to
use
of
multiple
strains
necessitates
an
efficient
population
strategy.
Herein,
we
report
circuit-guided
synthetic
acclimation
as
a
strategy
improve
biochemical
production
by
microbial
consortium.
We
designed
consortium
comprising
alginate-utilizing
Vibrio
sp.
dhg
and
3-hydroxypropionic
acid
(3-HP)-producing
Escherichia
coli
direct
conversion
alginate
3-HP.
introduced
genetic
circuit,
named
“Population
guider”,
E.
strain,
which
degrades
ampicillin
only
when
3-HP
is
produced.
In
presence
selection
pressure,
was
successfully
acclimated
increased
4.3-fold
compared
that
simple
co-culturing
during
48-h
fermentation.
believe
this
concept
useful
development
robust
consortium-based
bioprocesses.
Chemical Reviews,
Год журнала:
2022,
Номер
123(9), С. 5521 - 5570
Опубликована: Дек. 30, 2022
Metabolic
engineering
aims
to
improve
the
production
of
economically
valuable
molecules
through
genetic
manipulation
microbial
metabolism.
While
discipline
is
a
little
over
30
years
old,
advancements
in
metabolic
have
given
way
industrial-level
molecule
benefitting
multiple
industries
such
as
chemical,
agriculture,
food,
pharmaceutical,
and
energy
industries.
This
review
describes
design,
build,
test,
learn
steps
necessary
for
leading
successful
campaign.
Moreover,
we
highlight
major
applications
engineering,
including
synthesizing
chemicals
fuels,
broadening
substrate
utilization,
improving
host
robustness
with
focus
on
specific
case
studies.
Finally,
conclude
discussion
perspectives
future
challenges
related
engineering.
Biotechnology for Biofuels and Bioproducts,
Год журнала:
2024,
Номер
17(1)
Опубликована: Янв. 22, 2024
Abstract
Background
Classical
directed
evolution
is
a
powerful
approach
for
engineering
biomolecules
with
improved
or
novel
functions.
However,
it
traditionally
relies
on
labour-
and
time-intensive
iterative
cycles,
due
in
part
to
the
need
multiple
molecular
biology
steps,
including
DNA
transformation,
limited
screening
throughput.
Results
In
this
study,
we
present
an
ultrahigh
throughput
vivo
continuous
system
thermosensitive
inducible
tunability,
which
based
error-prone
polymerase
expression
modulated
by
engineered
thermal-responsive
repressor
cI857,
genomic
MutS
mutant
temperature-sensitive
defect
fixation
of
mutations
Escherichia
coli
.
We
demonstrated
success
platform
β-lactamase
as
model,
approximately
600-fold
increase
targeted
mutation
rate.
Furthermore,
was
combined
ultrahigh-throughput
methods
employed
evolve
α-amylase
resveratrol
biosynthetic
pathway.
After
rounds
enrichment,
48.3%
improvement
activity
identified
via
microfluidic
droplet
screening.
addition,
when
coupled
biosensor
pathway,
variant
1.7-fold
higher
production
selected
fluorescence-activated
cell
sorting.
Conclusions
mutagenesis
developed
rapid
enzymes
pathways.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 3, 2025
Yeast
sex-hormone
whole-cell
biosensors
are
analytical
tools
characterized
by
long-time
storage
and
low
production
cost.
We
engineered
compact
β-estradiol
in
S.
cerevisiae
cells
leveraging
short
(20-nt
long)
operators
bound
the
fusion
protein
LexA-ER-VP64—where
ER
is
human
estrogen
receptor
VP64
a
strong
viral
activation
domain.
Our
best
showed
high
accuracy
since
their
recovery
concentration
ranged
between
97.13%
104.69%.
As
novelty,
we
built
on
top
of
them
testosterone
that
exploit
conversion
into
aromatase
enzyme—expressed
together
with
its
co-factor
CPR.
used
our
yeast
strains
to
evaluate
activity
through
fluorescence
measurements
without
need
for
purification.
Besides,
set
up
an
aromatase-inhibitors
evaluation
assay
measure
IC50
(half-maximal
inhibitory
concentration)
candidate
compounds
developed
screening
enzymes
metabolize
demands
only
fluorescence.
These
two
assays
allow
large
number
chemicals
proteins
fast
economic
fashion.
think
work
will
facilitate
considerably
throughput
discovery
new
drugs
unknown
metabolic
processes.
Journal of Advanced Research,
Год журнала:
2022,
Номер
49, С. 1 - 14
Опубликована: Сен. 12, 2022
Nitrogen
sources
play
an
essential
role
in
maintaining
the
physiological
and
biochemical
activity
of
bacteria.
metabolism,
which
is
core
microorganism
makes
bacteria
able
to
autonomously
respond
different
external
nitrogen
environments
by
exercising
complex
internal
regulatory
networks
help
them
stay
ideal
state.
Although
various
studies
have
been
put
forth
better
understand
this
regulation
Bacillus,
many
valuable
viewpoints
obtained,
these
views
need
be
presented
systematically
their
possible
applications
specified.The
intention
provide
a
deep
comprehensive
understanding
metabolism
important
industrial
microorganism,
thereby
apply
logic
synthetic
biology
improve
biosynthesis
competitiveness.
In
addition,
potential
researches
future
are
also
discussed.Understanding
meticulous
process
Bacillus
not
only
could
facilitate
research
on
metabolic
engineering
but
constructive
insights
inspiration
for
other
microorganisms.
ACS Synthetic Biology,
Год журнала:
2023,
Номер
12(2), С. 533 - 543
Опубликована: Фев. 1, 2023
Gallic
acid
is
a
prevalent
secondary
plant
metabolite
distinguished
as
one
of
the
most
effective
free-radical
scavengers
among
phenolic
acids.
This
compound
also
known
for
its
cytotoxic,
anti-inflammatory,
and
antimicrobial
activities.
Bulk
quantities
gallic
are
conventionally
produced
by
hydrolysis
tannins,
costly
environmentally
hazardous
process.
With
aim
to
develop
more
sustainable
approaches,
microbial
bioproduction
strategies
have
been
attempted
recently.
To
advance
synthetic
biology
metabolic
engineering
microorganisms
production,
we
characterize
here
transcription
factor-based
inducible
system
PpGalR/PPP_RS13150
that
responds
extracellular
in
dose-dependent
manner
Pseudomonas
putida
KT2440.
Surprisingly,
this
does
not
mediate
induction
when
used
non-native
host
background.
We
show
activation
requires
gallate
dioxygenase
activity
encoded
galA
gene.
The
4-oxalomesaconic
acid,
an
intermediate
acid-metabolism,
identified
effector
molecule
interacts
with
factor
GalR
mediating
gene
expression.
Introduction
along
galR
enables
development
biosensors
suitable
detection
monitoring
extracellularly
using
hosts
such
E.
coli
C.
necator.
Moreover,
P.
putida-based
biosensor's
applicability
demonstrated
detecting
measuring
extracts
Camellia
sinensis
leaves.
study
reports
strategy,
which
can
be
applied
developing
bacterial
species
outside
genus.
