Circuit-guided population acclimation of a synthetic microbial consortium for improved biochemical production DOI Creative Commons

Chae Won Kang,

Hyun Gyu Lim,

Jaehyuk Won

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Nov. 7, 2022

Abstract Microbial consortia have been considered potential platforms for bioprocessing applications. However, the complexity in process control owing to use of multiple strains necessitates an efficient population strategy. Herein, we report circuit-guided synthetic acclimation as a strategy improve biochemical production by microbial consortium. We designed consortium comprising alginate-utilizing Vibrio sp. dhg and 3-hydroxypropionic acid (3-HP)-producing Escherichia coli direct conversion alginate 3-HP. introduced genetic circuit, named “Population guider”, E. strain, which degrades ampicillin only when 3-HP is produced. In presence selection pressure, was successfully acclimated increased 4.3-fold compared that simple co-culturing during 48-h fermentation. believe this concept useful development robust consortium-based bioprocesses.

Language: Английский

Metabolic Engineering: Methodologies and Applications DOI

Michael Volk,

Vinh Tran, Shih‐I Tan

et al.

Chemical Reviews, Journal Year: 2022, Volume and Issue: 123(9), P. 5521 - 5570

Published: Dec. 30, 2022

Metabolic engineering aims to improve the production of economically valuable molecules through genetic manipulation microbial metabolism. While discipline is a little over 30 years old, advancements in metabolic have given way industrial-level molecule benefitting multiple industries such as chemical, agriculture, food, pharmaceutical, and energy industries. This review describes design, build, test, learn steps necessary for leading successful campaign. Moreover, we highlight major applications engineering, including synthesizing chemicals fuels, broadening substrate utilization, improving host robustness with focus on specific case studies. Finally, conclude discussion perspectives future challenges related engineering.

Language: Английский

Citations

108
Engineering whole-cell microbial biosensors: Design principles and applications in monitoring and treatment of heavy metals and organic pollutants DOI
Changjiang Liu, Huan Yu, Baocai Zhang

et al.

Biotechnology Advances, Journal Year: 2022, Volume and Issue: 60, P. 108019 - 108019

Published: July 16, 2022

Language: Английский

Citations

77
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications DOI

Wenwen Yu,

Xianhao Xu, Ke Jin

et al.

Biotechnology Advances, Journal Year: 2022, Volume and Issue: 62, P. 108077 - 108077

Published: Dec. 9, 2022

Language: Английский

Citations

45
Metabolic engineering and fermentation optimization strategies for producing organic acids of the tricarboxylic acid cycle by microbial cell factories DOI
Shenghu Zhou,

Nana Ding,

Runhua Han

et al.

Bioresource Technology, Journal Year: 2023, Volume and Issue: 379, P. 128986 - 128986

Published: March 30, 2023

Language: Английский

Citations

27
Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis DOI

Minrui Lu,

Yuanyuan Sha,

Vinod Kumar

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 72, P. 108339 - 108339

Published: March 18, 2024

Language: Английский

Citations

13
Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering DOI Creative Commons

Shuaili Chen,

Zhanhao Yang,

Zeyuan Zhong

et al.

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: Jan. 22, 2024

Abstract Background Classical directed evolution is a powerful approach for engineering biomolecules with improved or novel functions. However, it traditionally relies on labour- and time-intensive iterative cycles, due in part to the need multiple molecular biology steps, including DNA transformation, limited screening throughput. Results In this study, we present an ultrahigh throughput vivo continuous system thermosensitive inducible tunability, which based error-prone polymerase expression modulated by engineered thermal-responsive repressor cI857, genomic MutS mutant temperature-sensitive defect fixation of mutations Escherichia coli . We demonstrated success platform β-lactamase as model, approximately 600-fold increase targeted mutation rate. Furthermore, was combined ultrahigh-throughput methods employed evolve α-amylase resveratrol biosynthetic pathway. After rounds enrichment, 48.3% improvement activity identified via microfluidic droplet screening. addition, when coupled biosensor pathway, variant 1.7-fold higher production selected fluorescence-activated cell sorting. Conclusions mutagenesis developed rapid enzymes pathways.

Language: Английский

Citations

12
Efficient sex hormone biosensors in Saccharomyces cerevisiae cells to evaluate human aromatase activity and inhibition DOI Creative Commons
Jie Wu, Matthias Bureik, Mario Andrea Marchisio

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Yeast sex-hormone whole-cell biosensors are analytical tools characterized by long-time storage and low production cost. We engineered compact β-estradiol in S. cerevisiae cells leveraging short (20-nt long) operators bound the fusion protein LexA-ER-VP64—where ER is human estrogen receptor VP64 a strong viral activation domain. Our best showed high accuracy since their recovery concentration ranged between 97.13% 104.69%. As novelty, we built on top of them testosterone that exploit conversion into aromatase enzyme—expressed together with its co-factor CPR. used our yeast strains to evaluate activity through fluorescence measurements without need for purification. Besides, set up an aromatase-inhibitors evaluation assay measure IC50 (half-maximal inhibitory concentration) candidate compounds developed screening enzymes metabolize demands only fluorescence. These two assays allow large number chemicals proteins fast economic fashion. think work will facilitate considerably throughput discovery new drugs unknown metabolic processes.

Language: Английский

Citations

1
Understanding and application of Bacillus nitrogen regulation: A synthetic biology perspective DOI Creative Commons

Hehe He,

Youran Li, Liang Zhang

et al.

Journal of Advanced Research, Journal Year: 2022, Volume and Issue: 49, P. 1 - 14

Published: Sept. 12, 2022

Nitrogen sources play an essential role in maintaining the physiological and biochemical activity of bacteria. metabolism, which is core microorganism makes bacteria able to autonomously respond different external nitrogen environments by exercising complex internal regulatory networks help them stay ideal state. Although various studies have been put forth better understand this regulation Bacillus, many valuable viewpoints obtained, these views need be presented systematically their possible applications specified.The intention provide a deep comprehensive understanding metabolism important industrial microorganism, thereby apply logic synthetic biology improve biosynthesis competitiveness. In addition, potential researches future are also discussed.Understanding meticulous process Bacillus not only could facilitate research on metabolic engineering but constructive insights inspiration for other microorganisms.

Language: Английский

Citations

34
Microbial cell factories for the production of flavonoids–barriers and opportunities DOI

Hongbiao Li,

Yunbin Lyv,

Shenghu Zhou

et al.

Bioresource Technology, Journal Year: 2022, Volume and Issue: 360, P. 127538 - 127538

Published: June 28, 2022

Language: Английский

Citations

33
Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid DOI Creative Commons
Ingrida Kutraite, Naglis Malys

ACS Synthetic Biology, Journal Year: 2023, Volume and Issue: 12(2), P. 533 - 543

Published: Feb. 1, 2023

Gallic acid is a prevalent secondary plant metabolite distinguished as one of the most effective free-radical scavengers among phenolic acids. This compound also known for its cytotoxic, anti-inflammatory, and antimicrobial activities. Bulk quantities gallic are conventionally produced by hydrolysis tannins, costly environmentally hazardous process. With aim to develop more sustainable approaches, microbial bioproduction strategies have been attempted recently. To advance synthetic biology metabolic engineering microorganisms production, we characterize here transcription factor-based inducible system PpGalR/PPP_RS13150 that responds extracellular in dose-dependent manner Pseudomonas putida KT2440. Surprisingly, this does not mediate induction when used non-native host background. We show activation requires gallate dioxygenase activity encoded galA gene. The 4-oxalomesaconic acid, an intermediate acid-metabolism, identified effector molecule interacts with factor GalR mediating gene expression. Introduction along galR enables development biosensors suitable detection monitoring extracellularly using hosts such E. coli C. necator. Moreover, P. putida-based biosensor's applicability demonstrated detecting measuring extracts Camellia sinensis leaves. study reports strategy, which can be applied developing bacterial species outside genus.

Language: Английский

Citations

22