Gallic
acid
(GA),
widely
used
in
food
and
medicine
production
industries.
Herein,
a
kind
of
newly
synthesized
cobalt-nitrogen
co-doped
carbon
dots
(Co,N-CDs)
with
dual
functional
properties
oxidase-like
activity
photoluminescence
property
could
oxidize
the
3,3',5,5'-tetramethylbenzidine
(TMB)
to
oxTMB
new
absorption
peak
at
653
nm.
In
addition,
it
was
found
that
performed
fluorescence-quenching
effect
on
Co,N-CDs
440
nm
attributed
FRET
(Fluorescence
resonance
energy
transfer).
Reductive
GA
reduce
TMB,
weaken
blue
color,
restore
fluorescence
Co,N-CDs.
Thus,
colorimetric
fluorometric
method
for
dual-mode
detection
established.
The
synthesis
dual-signal
probe
completed
single
environmental
precursor
just
one
step.
Simultaneously,
reduced
limits,
broadened
linear
range
improved
anti-interference
ability.
This
exhibited
satisfactory
application
prospect
actual
tea
beverages.
Critical Reviews in Biotechnology,
Год журнала:
2024,
Номер
unknown, С. 1 - 20
Опубликована: Июль 14, 2024
Microbial-based
bio-refining
promotes
the
development
of
a
biotechnology
revolution
to
encounter
and
tackle
enormous
challenges
in
petroleum-based
chemical
production
by
biomanufacturing,
biocomputing,
biosensing.
Nevertheless,
microbial
metabolic
homeostasis
is
often
incompatible
with
efficient
synthesis
bioproducts
mainly
due
to:
inefficient
flow,
robust
central
metabolism,
sophisticated
network,
inevitable
environmental
perturbation.
Therefore,
this
review
systematically
summarizes
how
optimize
strengthening
flux
for
improving
biotransformation
turnover,
redirecting
direction
rewiring
bypass
pathway,
reprogramming
network
boosting
substrate
utilization.
Future
directions
are
also
proposed
providing
constructive
guidance
on
industrial
biotechnology.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 23, 2024
Histamine
is
a
biogenic
amine;
its
level
indicates
food
quality,
as
elevated
levels
cause
poisoning.
Therefore,
monitoring
at
each
step
during
processing
until
it
reaches
the
consumer
crucial,
but
current
techniques
are
complicated
and
time-consuming.
Here,
we
designed
Pseudomonas
putida
whole-cell
biosensor
using
histamine-responsive
genetic
element
expressing
fluorescent
protein
in
presence
of
cognate
target.
We
improved
performance
proposed
by
optimizing
chassis,
regulatory
element,
reporter
gene.
A
sensitive
rapid
variant
was
obtained
with
limit
detection
(LOD)
0.39
ppm,
manifesting
linear
response
(R2
=
0.98)
from
0.28
to
18
ppm
90
min.
The
showed
minimal
cross-reactivity
other
amines
amino
acids
prevalent
food,
making
highly
specific.
effectively
quantified
histamine
spiked
fish,
prawn,
wine
samples
satisfactory
recovery.
Additionally,
colorimetric
sensor
PAlacZ
developed
enabling
quantification
seafood
via
smartphone
application,
an
LODgray
0.23
exhibiting
0
2.24
ppm.
Overall,
this
study
reports
efficient,
specific,
strong
potential
for
on-site
histamine,
ensuring
safety.
Microbial Cell Factories,
Год журнала:
2023,
Номер
22(1)
Опубликована: Окт. 30, 2023
Lactic
acid
is
one
of
the
most
important
organic
acids,
with
various
applications
in
food,
beverage,
pharmaceutical,
cosmetic,
and
chemical
industries.
Optically
pure
forms
L-
D-lactic
produced
via
microbial
fermentation
play
an
role
synthesis
biodegradable
polylactic
acid.
Alternative
substrates,
including
by-products
residues
from
agro-food
industry,
provide
a
cost-effective
solution
for
lactic
production
are
promising
avenue
circular
economy.In
this
study,
transcription
factor
(TF)-based
whole-cell
biosensor
strategy
was
developed
determination.
It
cross
validated
commonly
used
high-performance
liquid
chromatography
enzymatic
methods.
The
utility
biosensors
as
efficient
analytical
tool
demonstrated
by
their
application
determination
improvement.
We
explored
ability
Lacticaseibacillus
paracasei
subsp.
paracasei,
Lactobacillus
delbrueckii
lactis,
amylovorus
to
biosynthesize
optically
L-lactic
acid,
or
mixture
both
organic-rich
residual
fraction
(ORRF),
waste
glucose
syrup
wheat
starch.
complex
industrial
allowed
without
additional
pretreatment
obtaining
yields
0.5
0.9
Cmol/Cmol
glucose.This
study
highlights
whole
cell
D-forms
L.
amylovorus,
respectively,
using
production,
ORRF.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(47), С. 18727 - 18735
Опубликована: Ноя. 16, 2024
Gentisic
acid
is
a
secondary
plant
metabolite,
known
for
its
health
benefits,
not
only
widely
used
as
supplement
but
also
implicated
potential
biomarker
cancer-associated
metabolism
alterations.
To
advance
bioproduction
and
detection
of
this
compound
or
derivatives,
cell-based
approaches
have
become
interest
in
recent
years.
However,
the
lack
tools
high-throughput
gentisic
monitoring
compound-metabolizing
organism
screening
limits
progress
area.
Here,
we
analyzed
gene
cluster
responsible
Cupriavidus
necator
H16.
The
transcriptional
regulator
GtdR-based
inducible
expression
system
CnGtdR/PgtdA
was
elucidated,
showing
that
it
activated
when
C.
cells
were
subjected
to
acid.
Subsequently,
3-maleylpyruvic
identified
primary
inducer
system.
Furthermore,
genes
gtdA
gtdT,
encoding
gentisate
1,2-dioxygenase
MFS
transporter,
shown
be
essential
activation
presence
with
GtdA
enabling
conversion
phenolic
into
inducer.
CnGtdRAT/PgtdA-based
employed
develop
whole-cell
biosensor
intracellular
extracellular
acid-inducible
demonstrated
by
application
metabolic
pathway
research,
highly
unstable
acid,
development
biosensors
determination
In
addition,
utility
emphasized
cancer
urine
samples.
BIO Web of Conferences,
Год журнала:
2024,
Номер
142, С. 03018 - 03018
Опубликована: Янв. 1, 2024
There
is
an
increasing
need
for
precise
regulation
of
complex
metabolic
pathways
in
the
cell
factory
development,
and
thus
discovery
new
cis
-regulatory
elements
responding
trans
especially
attracting
attention
scientific
researchers.
Previously,
endophyte
S.
rubidaea
W12-1
was
isolated
from
root
Glycyrrhiza
uralensis
Fisch.
derived
northwest
China.
Here,
it
found
that
prodigiosin,
a
natural
red
pigment
belonging
to
class
alkaloids
with
multiple
pharmacological
activities,
produced
by
under
specific
conditions
growth
stages,
indicating
there
should
be
interesting
inducible
promoters
regulatory
factors
.
Therefore,
whole
genome
sequencing
analysis
were
operated
on
map
prodigiosin
biosynthesis
gene
cluster,
called
pig
its
upstream
promoter
Ppig.
After
DNA
pull-down
assay
proteomics,
22
transcription
factor
candidates
being
expected
interact
Ppig
screened
out.
Among
them,
AstD,
DinB,
GlgP,
GlmS,
FimA,
MreB,
PchP,
RplB,
RpoB,
RpsD,
SucB,
TopA,
TorZ,
TPA,
YaaA,
YeiP
confirmed
transcriptional
repressors
according
vitro
experiments
which
expression
vectors
(pRS415-PgapA-gene
candidate-Ppig-
eGFP
)
constructed
individually
transferred
into
Escherichia
coli
followed
cultivation
fluorescence
detection
engineered
strains.
Meanwhile,
L-proline,
compound
metabolism,
could
significantly
enhance
repression
SucB
alleviate
TopA
TorZ
Ppig-
,
while
malonyl-CoA,
second
CgtA
In
summary,
this
study
improved
understanding
mechanism
metabolism
Serratia
provided
potential
engineering
high-throughput
screening
target
strains,
contributed
enriching
genetic
components
microorganisms
synthetic
biology.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 29, 2023
Abstract
Bioengineers
increasingly
rely
on
ligand-inducible
transcription
regulators
for
chemical-responsive
control
of
gene
expression,
yet
the
number
available
is
limited.
Novel
can
be
mined
from
genomes,
but
an
inadequate
understanding
their
DNA
specificity
complicates
genetic
design.
Here
we
present
Snowprint,
a
simple
powerful
bioinformatic
tool
predicting
regulator:DNA
interactions.
Benchmarking
results
demonstrate
that
Snowprint
predictions
are
significantly
similar
>45%
experimentally
validated
regulator:operator
pairs
organisms
across
nine
phyla
and
span
five
distinct
structural
families.
We
then
use
to
design
promoters
33
previously
uncharacterized
sourced
diverse
phylogenies,
which
28
were
shown
influence
expression
24
produced
>20-fold
signal-to-noise
ratio.
A
panel
newly
domesticated
screened
response
biomanufacturing-relevant
compounds,
yielding
new
sensors
polyketide
(olivetolic
acid),
terpene
(geraniol),
steroid
(ursodiol),
alkaloid
(tetrahydropapaverine)
with
induction
ranges
up
10.7-fold.
represents
unique,
generalizable
greatly
facilitates
discovery
transcriptional
bioengineering
applications.
Figure
