Опубликована: Янв. 1, 2024
Язык: Английский
MicrobiologyOpen, Год журнала: 2024, Номер 13(2)
Опубликована: Март 31, 2024
Abstract Microbial products are essential for developing various therapeutic agents, including antibiotics, anticancer drugs, vaccines, and enzymes. Genetic engineering techniques, functional genomics, synthetic biology unlock previously uncharacterized natural products. This review highlights major advances in microbial biotechnology, focusing on gene‐based technologies medical applications.
Язык: Английский
Процитировано
13
Microbiology Spectrum, Год журнала: 2024, Номер 12(2)
Опубликована: Янв. 9, 2024
Fungal secondary metabolites (SMs) contribute to the diversity of fungal ecological communities, niches, and lifestyles. Many SMs have one or more medically industrially important activities (e.g., antifungal, antibacterial, antitumor). The genes necessary for SM biosynthesis are typically located right next each other in genome known as biosynthetic gene clusters (BGCs). However, whether bioactivity can be predicted from specific attributes BGCs remains an open question. We adapted machine learning models that bacterial BGC data with accuracies high 80% data. trained our predict cytotoxic/antitumor on two sets: (i) (data set comprised 314 BGCs) (ii) (314 (1,003 BGCs). found had balanced between 51% 68%, whereas training 56% 68%. low prediction accuracy bioactivities likely stems small size set; this lack data, coupled finding including did not substantially change currently limits application approaches studies. With >15,000 characterized SMs, millions putative genomes, increased demand novel drugs, efforts systematically link urgently needed.IMPORTANCEFungi key sources natural products iconic penicillin statins. DNA sequencing has revealed there pathways but chemical structures >99% produced by these remain unknown. used artificial intelligence diverse pathways. predictions were generally low, because only very few known. products, present study suggests is urgent need identify pathways, their bioactivities.
Язык: Английский
Процитировано
10
Communications Chemistry, Год журнала: 2024, Номер 7(1)
Опубликована: Апрель 18, 2024
Abstract Natural products are small molecules synthesized by fungi, bacteria and plants, which historically have had a profound effect on human health quality of life. These natural evolved over millions years resulting in specific biological functions that may be interest for pharmaceutical, agricultural, or nutraceutical use. Often need to structurally modified make them suitable applications. Combinatorial biosynthesis is method alter the composition enzymes needed synthesize product diversified molecules. In this review we discuss different approaches combinatorial via engineering fungal biosynthetic pathways. We highlight knowledge gained from these studies provide examples new-to-nature bioactive molecules, including using combinations non-fungal enzymes.
Язык: Английский
Процитировано
7
Biosensors and Bioelectronics, Год журнала: 2024, Номер 249, С. 116004 - 116004
Опубликована: Янв. 5, 2024
Язык: Английский
Процитировано
6
Critical Reviews in Biotechnology, Год журнала: 2024, Номер unknown, С. 1 - 21
Опубликована: Авг. 12, 2024
Natural products have long served as critical raw materials in chemical and pharmaceutical manufacturing, primarily which can provide superior scaffolds or intermediates for drug discovery development. Over the last century, natural contributed to more than a third of therapeutic production. However, traditional methods producing drugs from become less efficient expensive over past few decades. The combined utilization genome mining synthetic biology based on sequencing, bioinformatics tools, big data analytics, genetic engineering, metabolic systems promises counter this trend. Here, we reviewed recent (2020–2023) examples used resolve challenges production products, such variety, poor efficiency, low yield. Additionally, emerging design principles, building strategies its application prospects NPs synthesis also been discussed.
Язык: Английский
Процитировано
6
Microbial Biotechnology, Год журнала: 2024, Номер 17(8)
Опубликована: Июль 29, 2024
Abstract Marine microorganisms are increasingly recognized as primary producers of marine secondary metabolites, drawing growing research interest. Many these organisms unculturable, posing challenges for study. Metagenomic techniques enable on unculturable microorganisms, identifying various biosynthetic gene clusters (BGCs) related to microbial thereby unveiling their secrets. This review comprehensively analyses metagenomic methods used in discovering highlighting tools commonly employed BGC identification, and discussing the potential this field. It emphasizes key role metagenomics particularly sponges tunicates. The also explores current limitations studying metabolites through metagenomics, noting how long‐read sequencing technologies evolution computational biology offer more possibilities discovery. Furthermore, development synthetic allows experimental validation computationally identified BGCs, showcasing vast mining metabolites.
Язык: Английский
Процитировано
5
Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108532 - 108532
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Cell Systems, Год журнала: 2025, Номер 16(3), С. 101237 - 101237
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Microbial Biotechnology, Год журнала: 2025, Номер 18(3)
Опубликована: Март 1, 2025
ABSTRACT The combination of artificial intelligence (AI) with microbial technology marks the start a major transformation, improving applications throughout biotechnology, especially in healthcare. With capability AI to process vast amounts biological big data, advanced allows for comprehensive understanding complex systems, advancing disease diagnosis, treatment and development therapeutics. This mini review explores impact AI‐integrated technologies healthcare, highlighting advancements biomarker‐based therapeutics production therapeutic compounds. exploration promises significant improvements design implementation health‐related solutions, steering new era biotechnological applications.
Язык: Английский
Процитировано
0
Chemical Reviews, Год журнала: 2025, Номер unknown
Опубликована: Март 21, 2025
Synthetic biology has played an important role in the renaissance of natural products research during post-genomics era. The development and integration new tools have transformed workflow product discovery engineering, generating multidisciplinary interest field. In this review, we summarize recent developments biosynthesis from three different aspects. First, advances bioinformatics, experimental, analytical to identify associated with predicted biosynthetic gene clusters (BGCs) will be covered. This followed by extensive review on heterologous expression bacterial, fungal plant organisms. native host-independent paradigm identification, pathway characterization, enzyme is where synthetic most prominent role. Lastly, strategies engineer pathways for structural diversification complexity generation discussed, including assembly-line megasynthase precursor-directed modification, combinatorial biosynthesis.
Язык: Английский
Процитировано
0