Chemical Science, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
BenzoTag, a self-labeling protein developed using molecular evolution, enables wash-free labeling in live cells seconds low nanomolar dye concentrations.
Язык: Английский
Chemical Society Reviews, Год журнала: 2024, Номер 53(15), С. 7681 - 7741
Опубликована: Янв. 1, 2024
This review summarizes insights into colorant selection and signal mechanisms for the development of colorimetric sensing POC sensors.
Язык: Английский
Процитировано
37
Chemical Society Reviews, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
In this review, recent progress in tetrazine-based fluorogenic probes for bioorthogonal chemistry is comprehensively analyzed, with a focus on advancements probe design and their imaging applications molecular processes live cells.
Язык: Английский
Процитировано
2
TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 174, С. 117685 - 117685
Опубликована: Апрель 2, 2024
Язык: Английский
Процитировано
11
Journal of the American Chemical Society, Год журнала: 2025, Номер unknown
Опубликована: Янв. 12, 2025
Covalent modification of cell membranes has shown promise for tumor imaging and therapy. However, existing membrane labeling techniques face challenges such as slow kinetics poor selectivity cancer cells, leading to off-target effects suboptimal in vivo efficacy. Here, we present an enzyme-triggered self-immobilization strategy, termed E-SIM, which enables rapid selective with bioorthogonal trans-cycloctene (TCO) handles vivo. E-SIM utilizes P-TCO, alkaline phosphatase (ALP) responsive quinone methide (QM) precursor a TCO group, facilitating the conjugation high-density onto via proximity labeling. These groups then react efficiently tetrazine (Tz)-bearing reporters fast reaction, resulting significant enrichment various sizes modalities on membranes. We demonstrate efficacy reaction pretargeted multimodality tumors Notably, achieve efficient installation Tz-modified Renilla luciferase cells vivo, thereby offering highly sensitive bioluminescence signals detecting guiding surgical removal small human HepG2 liver peritoneal metastases. represents robust tool precise complex environments, feasible multimodal applications.
Язык: Английский
Процитировано
1
Coordination Chemistry Reviews, Год журнала: 2024, Номер 510, С. 215850 - 215850
Опубликована: Апрель 8, 2024
Язык: Английский
Процитировано
5
Chemistry - A European Journal, Год журнала: 2024, Номер 30(40)
Опубликована: Май 13, 2024
1,2,4-triazines are a valuable class of heterodienes that can be employed in inverse electron-demand Diels-Alder reactions. However, their broader application bioorthogonal chemistry is limited due to low reactivity. This article focuses on 3-(trifluoromethyl)-1,2,4-triazines, which efficiently prepared one-pot reaction from NH-1,2,3-triazoles. These triazines highly reactive reactions with strained cyclooctenes, giving second-order rate constants as high 230 M
Язык: Английский
Процитировано
4
Chinese Chemical Letters, Год журнала: 2024, Номер 36(5), С. 110082 - 110082
Опубликована: Июнь 3, 2024
Язык: Английский
Процитировано
4
JACS Au, Год журнала: 2024, Номер 4(7), С. 2687 - 2694
Опубликована: Июль 5, 2024
Hydrogen sulfide (H2S) is a gaseous signaling molecule, exerting crucial regulatory functions in organelles and cellular environments. H2S exhibits high therapeutic potential synergistic effects with other drugs, its potency notably enhanced through organelle-specific targeting. Yet, the navigation of light-activated donors to specific remains absent. Here, we report first photocage that simultaneously delivers payload subcellular precision mitochondria live human cells using tissue-penetrating near-infrared light as trigger. The fluorogenic enables real-time monitoring process, demonstrate concurrent uncaging combination fluorescence microscopy mitochondria-specific fluorescent probes. We anticipate these photocages will permit precise delivery H2S-drug combinations exceptional spatiotemporal control, thereby driving harnessing known discovery novel strategies.
Язык: Английский
Процитировано
4
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер 330, С. 125743 - 125743
Опубликована: Янв. 11, 2025
Язык: Английский
Процитировано
0
Chemical Science, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Tetrazine-based fluorogenic labels are widely utilized in medical and biological studies, exhibiting substantial fluorescence enhancement (FE) following tetrazine degradation through bio-orthogonal reactions. However, the underlying mechanisms driving this response remain only partially resolved, particularly regarding diminished FE efficiency deep-red near-infrared (NIR) regions. This knowledge gap has impeded efforts to optimize these for extended emission wavelengths improved ratios. review offers a photophysical perspective, discussing quenching pathways (i.e., energy flows charge separation) that regulate properties exhibited various types of labels. Moreover, work examines emerging role intramolecular rotations certain tetrazine-based structures integration additional quencher units. The proposed alternative channel potential surpass traditional wavelength constraints while achieving FE. By examining mechanisms, aims advance understanding tetrazine-functionalized provide guiding principles their future design practical applications.
Язык: Английский
Процитировано
0