Multiplexed No-Wash Cellular Imaging using BenzoTag, an Evolved Self-Labeling Protein DOI Creative Commons
Bryan J. Lampkin, Benjamin J. Goldberg, Joshua A. Kritzer

et al.

Chemical Science, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

BenzoTag, a self-labeling protein developed using molecular evolution, enables wash-free labeling in live cells seconds low nanomolar dye concentrations.

Language: Английский

Colorimetric sensing for translational applications: from colorants to mechanisms DOI
Zhicheng Jin, Wonjun Yim, Maurice Retout

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(15), P. 7681 - 7741

Published: Jan. 1, 2024

This review summarizes insights into colorant selection and signal mechanisms for the development of colorimetric sensing POC sensors.

Language: Английский

Citations

37
Design strategies for tetrazine fluorogenic probes for bioorthogonal imaging DOI
Aiwen Yu, Xinyu He, Tianruo Shen

et al.

Chemical Society Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

In this review, recent progress in tetrazine-based fluorogenic probes for bioorthogonal chemistry is comprehensively analyzed, with a focus on advancements probe design and their imaging applications molecular processes live cells.

Language: Английский

Citations

2
Recent progress of molecular fluorescent probes with multi-recognition sites enable sensitive and selective analysis DOI

Haifeng Ge,

Qiaozhen Ye,

Tenglong Zou

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 174, P. 117685 - 117685

Published: April 2, 2024

Language: Английский

Citations

11
Pretargeted Multimodal Tumor Imaging by Enzymatic Self-Immobilization Labeling and Bioorthogonal Reaction DOI

Yinxing Miao,

Yuqi Wang, Yefeng Chen

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 12, 2025

Covalent modification of cell membranes has shown promise for tumor imaging and therapy. However, existing membrane labeling techniques face challenges such as slow kinetics poor selectivity cancer cells, leading to off-target effects suboptimal in vivo efficacy. Here, we present an enzyme-triggered self-immobilization strategy, termed E-SIM, which enables rapid selective with bioorthogonal trans-cycloctene (TCO) handles vivo. E-SIM utilizes P-TCO, alkaline phosphatase (ALP) responsive quinone methide (QM) precursor a TCO group, facilitating the conjugation high-density onto via proximity labeling. These groups then react efficiently tetrazine (Tz)-bearing reporters fast reaction, resulting significant enrichment various sizes modalities on membranes. We demonstrate efficacy reaction pretargeted multimodality tumors Notably, achieve efficient installation Tz-modified Renilla luciferase cells vivo, thereby offering highly sensitive bioluminescence signals detecting guiding surgical removal small human HepG2 liver peritoneal metastases. represents robust tool precise complex environments, feasible multimodal applications.

Language: Английский

Citations

1
Progress and Perspective of Atherosclerosis-Targeted Molecular Probes: From Precise Imaging to Auxiliary Diagnosis and Treatment DOI

Cong Hu,

Zhipengjun Zhang,

Shanyi Sun

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 510, P. 215850 - 215850

Published: April 8, 2024

Language: Английский

Citations

5
Bioorthogonal Cycloadditions of C3‐Trifluoromethylated 1,2,4‐Triazines with trans‐Cyclooctenes DOI Creative Commons
Veronika Šlachtová, Vladimir Motornov, Petr Beier

et al.

Chemistry - A European Journal, Journal Year: 2024, Volume and Issue: 30(40)

Published: May 13, 2024

1,2,4-triazines are a valuable class of heterodienes that can be employed in inverse electron-demand Diels-Alder reactions. However, their broader application bioorthogonal chemistry is limited due to low reactivity. This article focuses on 3-(trifluoromethyl)-1,2,4-triazines, which efficiently prepared one-pot reaction from NH-1,2,3-triazoles. These triazines highly reactive reactions with strained cyclooctenes, giving second-order rate constants as high 230 M

Language: Английский

Citations

4
Rationally designed an innovative proximity labeling near-infrared fluorogenic probe for imaging of peroxynitrite in acute lung injury DOI
Dandan Tang,

Ningge Xu,

Yuyang Fu

et al.

Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: 36(5), P. 110082 - 110082

Published: June 3, 2024

Language: Английский

Citations

4
Concurrent Subcellular Delivery of Hydrogen Sulfide and a Payload with Near-Infrared Light DOI Creative Commons

Katarzyna Hanc,

Hana Janeková, Peter Štacko

et al.

JACS Au, Journal Year: 2024, Volume and Issue: 4(7), P. 2687 - 2694

Published: July 5, 2024

Hydrogen sulfide (H2S) is a gaseous signaling molecule, exerting crucial regulatory functions in organelles and cellular environments. H2S exhibits high therapeutic potential synergistic effects with other drugs, its potency notably enhanced through organelle-specific targeting. Yet, the navigation of light-activated donors to specific remains absent. Here, we report first photocage that simultaneously delivers payload subcellular precision mitochondria live human cells using tissue-penetrating near-infrared light as trigger. The fluorogenic enables real-time monitoring process, demonstrate concurrent uncaging combination fluorescence microscopy mitochondria-specific fluorescent probes. We anticipate these photocages will permit precise delivery H2S-drug combinations exceptional spatiotemporal control, thereby driving harnessing known discovery novel strategies.

Language: Английский

Citations

4
Rational design of hypochlorous acid-activatable fluorescent probe for diagnostic imaging and therapeutic evaluation in breast cancer recurrence DOI
Gang Nie, Wenjie Liang, Jun Wang

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 330, P. 125743 - 125743

Published: Jan. 11, 2025

Language: Английский

Citations

0
Unveiling the photophysical mechanistic mysteries of tetrazine-functionalized fluorogenic labels DOI Creative Commons
Tianruo Shen, Xiaogang Liu

Chemical Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Tetrazine-based fluorogenic labels are widely utilized in medical and biological studies, exhibiting substantial fluorescence enhancement (FE) following tetrazine degradation through bio-orthogonal reactions. However, the underlying mechanisms driving this response remain only partially resolved, particularly regarding diminished FE efficiency deep-red near-infrared (NIR) regions. This knowledge gap has impeded efforts to optimize these for extended emission wavelengths improved ratios. review offers a photophysical perspective, discussing quenching pathways (i.e., energy flows charge separation) that regulate properties exhibited various types of labels. Moreover, work examines emerging role intramolecular rotations certain tetrazine-based structures integration additional quencher units. The proposed alternative channel potential surpass traditional wavelength constraints while achieving FE. By examining mechanisms, aims advance understanding tetrazine-functionalized provide guiding principles their future design practical applications.

Language: Английский

Citations

0