Analytical Chemistry,
Год журнала:
2021,
Номер
93(48), С. 16105 - 16112
Опубликована: Ноя. 19, 2021
Cancer
is
a
serious
threat
to
human
health,
and
there
an
urgent
need
develop
new
treatment
methods
overcome
it.
Organelle
targeting
therapy,
as
highly
effective
less
toxic
side
effect
strategy,
has
great
research
significance
development
prospects.
Being
essential
organelle,
the
Golgi
apparatus
plays
particularly
major
role
in
growth
of
cancer
cells.
Acting
indispensable
expressed
antioxidant
cells,
glutathione
(GSH)
also
contributes
greatly
during
oxidative
stress.
Therefore,
it
counts
for
much
track
changes
GSH
concentration
monitoring
occurrence
tumor
exploring
Golgi-targeted
therapy
extremely
important
cancer.
In
this
work,
we
designed
synthesized
simple
Golgi-targeting
fluorescent
probe
GT-GSH
accurately
detecting
GSH.
The
reacting
with
decomposes
substances
Golgi,
thereby
killing
At
same
time,
ratiometric
can
detect
stress
high
sensitivity
selectivity
living
such
GSH-responsive
gives
method
accurate
Analytical Chemistry,
Год журнала:
2021,
Номер
93(4), С. 2510 - 2518
Опубликована: Янв. 20, 2021
Carbon
monoxide
(CO),
as
a
crucial
gasotransmitter,
is
endogenously
produced
by
the
degradation
of
heme
and
plays
critical
role
in
regulating
various
physiological
pathophysiological
processes
such
oxidative
stress.
Thus,
an
effective
fluorescent
probe
for
investigating
relationships
between
CO
stress
vivo
necessary.
In
this
paper,
ratiometric
near-infrared
(NIR)
(CP-CO)
based
on
coumarin-benzopyran
fluorophore
imaging
developed.
CP-CO
itself
displays
strong
coumarin
emission
due
to
its
spironolactone
structure.
After
reacted
with
PdCl2,
notable
enhancement
intensity
at
690
nm
can
be
found,
which
results
obvious
red
shift
(200
nm).
Moreover,
exhibits
high
sensitivity
toward
produces
ratio
(203-fold).
addition,
applied
monitoring
exogenous
endogenous
levels
HepG2
cells.
Furthermore,
fluorescence
zebrafish
performed
two-photon
excitation
along
excellent
penetration
ability.
Most
importantly,
visualize
upregulation
under
lipopolysaccharide
(LPS)-induced
model,
vividly
reveals
ability
elucidation
function
related
biological
events.
Analytical Chemistry,
Год журнала:
2021,
Номер
93(12), С. 5194 - 5200
Опубликована: Март 19, 2021
Benzopyrylium–coumarin
fluorescent
probes
for
sensing
hypochlorous
acid
(HClO)
or
sulfur
dioxide
(SO2)
are
unable
to
distinguish
between
HClO
and
SO2
because
the
two
compounds
can
react
with
4-position
of
benzopyrylium–coumarin
dyes
through
nucleophilic
attack.
In
current
work,
we
introduced
a
phenoxazine
moiety
dye
synthesize
new
probe
PBC1,
which
dually
sense
generate
distinct
fluorescence
signals
rapid
response
time
high
sensitivity
selectivity.
Moreover,
PBC1
was
also
successfully
utilized
detect
intracellular
in
HeLa
cells
zebrafish.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(33), С. 11573 - 11581
Опубликована: Авг. 9, 2022
Energy
deprivation
and
reduced
levels
of
hydrogen
sulfide
(H2S)
in
the
brain
is
closely
associated
with
Alzheimer's
disease
(AD).
However,
there
currently
no
fluorescent
probe
for
precise
exploration
both
H2S
adenosine
triphosphate
(ATP)
to
directly
demonstrate
their
relationship
dynamic
pattern
changes.
Herein,
we
developed
a
two-photon
probe,
named
AD-3,
simultaneously
image
endogenous
ATP
from
two
emission
channels
signals
live
rat
brains
AD.
The
achieved
excellent
selectivity
good
detection
linearity
0-100
μM
concentration
range
2-5
mM
range,
respectively,
limit
0.19
0.01
ATP.
Fluorescence
imaging
cells
reveals
that
such
could
successfully
apply
simultaneous
accurate
quantification
neuronal
cells.
Further
using
real-time
quantitative
polymerase
chain
reaction
Western
blots,
confirmed
regulates
synthesis
by
acting
on
cytochrome
C,
oxidase
subunit
3
complex
IV,
protein
6
I
mitochondrial
respiratory
chain.
Subsequently,
constructed
high-throughput
screening
platform
based
AD-3
rapidly
screen
potential
anti-AD
drugs
control
glutamate-stimulated
oxidative
stress
abnormal
levels.
Significantly,
was
found
capable
APP/PS1
mice,
AD
mouse
be
lower
than
wild-type
mice.
Angewandte Chemie International Edition,
Год журнала:
2022,
Номер
62(12)
Опубликована: Дек. 24, 2022
Quantifying
glutathione
(GSH)
in
cells
and
organisms
is
of
great
significance
for
understanding
the
mechanism
oxidative
stress
various
physiological
pathological
processes.
However,
quantification
by
fluorescence
bioimaging
living
tissues
has
much
stricter
requirements
than
"Petri
dish"-cultured
flat
plates.
Based
on
evaluation
electronic
structure
steric
hindrance-tuned
reactivity
phospha-substituted
rhodamine
with
GSH,
a
reversible
Förster
resonance
energy
transfer
(FRET)
probe
ZpSiP
distinct
performance
(Kd
=4.9
mM,
t1/2
=0.57
s,
k=81
M-1
s-1
)
developed
real
time
quantifying
GSH
cells.
Furthermore,
near-infrared
(NIR)
succeeded
sensitively
tracking
dynamics
bearing
tumors,
chronic
renal
failure,
liver
fibrosis
unveiling
related
We
believe
that
advance
chemistry
quantitative
analysis
methods
will
initiate
more
promising
progress
broad
applications.
