Modulation of Macrophages by In Situ Ligand Bridging

Advanced Functional Materials, Год журнала: 2023, Номер 33(16)

Опубликована: Фев. 8, 2023

Abstract Extracellular matrix (ECM) proteins containing cell‐attachable Arg‐Gly‐Asp (RGD) sequences exhibit variable bridging and non‐bridging in fibronectin‐collagen laminin‐collagen complexes that can regulate inflammation, tissue repair, wound healing. In this study, linking molecule‐mediated conjugation of 1D magnetic nanocylinders (MNCs) to material surfaces pre‐decorated with gold nanospheres (GNSs) is performed, thereby yielding RGD‐coated MNCs (RGD‐MNCs) over GNSs (RGD‐GNSs) a state. The RGD‐MNCs are drawn closer the RGD‐GNSs via field‐mediated compression molecules establish between them. Relative proportion optimized yield effective remote stimulation integrin binding variably bridged RGDs similar invariably used as control group. Remote manipulation RGD facilitates attachment structure assembly macrophages leads pro‐healing/anti‐inflammatory phenotype acquisition. contrast, non‐bridged inhibited macrophage acquired pro‐inflammatory phenotypes. use various nanomaterials constructing heterogeneous materials further offer modes switching understand dynamic integrin‐mediated modulation immunomodulatory responses, such foreign body

Язык: Английский

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Photonic control of image-guided ferroptosis cancer nanomedicine

Coordination Chemistry Reviews, Год журнала: 2023, Номер 500, С. 215532 - 215532

Опубликована: Ноя. 10, 2023

Язык: Английский

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Ligand Coupling and Decoupling Modulates Stem Cell Fate

Advanced Functional Materials, Год журнала: 2023, Номер 33(8)

Опубликована: Янв. 18, 2023

Abstract In natural microenvironment, various proteins containing adhesive ligands in fibrous and non‐fibrous structures dynamically couple decouple to regulate stem cell fate. Herein, materials presenting movably couplable are developed by grafting liganded gold nanoparticles (AuNPs) a substrate followed flexibly movable linear nanomaterials (MLNs) the via long bendable linker, thereby creating space between MLNs AuNPs decoupled state. Magnetic control of decreases this bending linker AuNPs. Remote ligand coupling stimulates integrin recruitment coupled ligands, non‐toxically facilitating focal adhesion, mechanosensing, potential differentiation cells, which is suppressed decoupling. Versatile tuning size, aspect ratio, distributions, can help decipher dynamic ligand‐coupling‐dependent fate advance regenerative therapies.

Язык: Английский

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Polymer Micropatches as Natural Killer Cell Engagers for Tumor Therapy

ACS Nano, Год журнала: 2023, Номер 17(16), С. 15918 - 15930

Опубликована: Авг. 11, 2023

Natural killer (NK) cell therapies have emerged as a potential therapeutic approach to various cancers. Their efficacy, however, is limited by their low persistence and anergy. Current approaches sustain NK in vivo include genetic modification, activation via pretreatment, or coadministration of supporting cytokines antibodies. Such exhibit efficacy vivo, part due the reversal effect within immunosuppressive tumor microenvironment off-target toxicity. Here, we report material-based address this challenge. Specifically, describe use polymeric micropatches platform for sustained, targeted cells, an referred microparticles engagers (MACE). Poly(lactide-co-glycolic) acid (PLGA) micropatches, 4-8 μm diameter surface-modified with receptor targeting antibodies, exhibited strong adhesion cells induced without need coadministered cytokines. The MACE was greater than that nanoparticles, attesting crucial role geometry cells. MACE-bound remained viable trans-endothelial migration antitumor activity vitro. activated T macrophages, dendritic Adoptive transfer NK-MACE also demonstrated superior mouse melanoma lung metastasis model compared unmodified Overall, offers simple, scalable, effective way activating represents attractive improve therapy.

Язык: Английский

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Nanotechnology: a contemporary therapeutic approach in combating infections from multidrug-resistant bacteria

Archives of Microbiology, Год журнала: 2023, Номер 205(2)

Опубликована: Янв. 11, 2023

Язык: Английский

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Antimicrobial carbon materials-based quantum dots: From synthesis strategies to antibacterial properties for diagnostic and therapeutic applications in wound healing

Coordination Chemistry Reviews, Год журнала: 2024, Номер 522, С. 216211 - 216211

Опубликована: Сен. 18, 2024

Язык: Английский

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Multimodal biomedical utility of polyaniline-based supramolecular nanomaterials

Chemical Engineering Journal, Год журнала: 2024, Номер 493, С. 152530 - 152530

Опубликована: Май 24, 2024

Язык: Английский

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Magnetic Control of Multiscale Ligand Nanoarchitecture Regulates Stem Cell Fate

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Abstract Native tissues exhibit hierarchical structures of anisotropically arranged extracellular matrix that dynamically regulate stem cells and tissue function. However, neither multiscale nano‐anisotropy nor dynamic anisotropy control have been reported. In this study, spherical or rod‐shaped gold small‐nanomaterials (at integrin receptor‐scale; tens nanometers) are coupled to the surface magnetic large‐nanomaterials focal adhesion complex‐scale; hundreds nanometers), with both showing constant areas at each respective scale. Each nanocomposite is flexibly conjugated substrate material densities, resulting in dual‐scale liganded nano‐anisotropies. Increasing aspect ratio nanomaterials nanometer‐scale dominantly promotes recruitment, adhesion, mechanotransduction, differentiation over nanometer‐scale. Such scale‐specific effects on cell regulation temporally regulated vitro vivo by physically raising lowering nanocomposites respectively inhibit stimulate curved surfaces modulating membrane bending. unprecedented “dynamic ligand anisotropy” can be independently engineered regarding scales, anisotropies, ligands elucidate cell‐material interactions allow for multimodal enhance tissue‐regenerative therapy.

Язык: Английский

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Pollen-like mesoporous silica nanoparticles facilitate macrophage-mediated anti-inflammatory response via physical contact cues in the osteoimmune microenvironment

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Photoswitchable Microgels for Dynamic Macrophage Modulation

Advanced Materials, Год журнала: 2022, Номер 34(49)

Опубликована: Окт. 21, 2022

Dynamic manipulation of supramolecular self-assembled structures is achieved irreversibly or under non-physiological conditions, thereby limiting their biomedical, environmental, and catalysis applicability. In this study, microgels composed azobenzene derivatives stacked via π-cation π-π interactions are developed that electrostatically stabilized with Arg-Gly-Asp (RGD)-bearing anionic polymers. Lateral swelling RGD-bearing occurs cis-azobenzene formation mediated by near-infrared-light-upconverted ultraviolet light, which disrupts intermolecular on the visible-light-absorbing upconversion-nanoparticle-coated materials. Real-time imaging molecular dynamics simulations demonstrate deswelling visible-light-mediated trans-azobenzene formation. Near-infrared light can induce in situ to increase RGD availability trigger release loaded interleukin-4, facilitates adhesion structure assembly linked pro-regenerative polarization host macrophages. contrast, visible decrease suppresses macrophage yields pro-inflammatory polarization. These exhibit high stability non-toxicity. Versatile use ligands protein delivery offer cytocompatible photoswitchable manipulability diverse cells.

Язык: Английский

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