Targeted and Long-Term Fluorescence Imaging of Plant Cytomembranes Using Main-Chain Charged Polyelectrolytes with Aggregation-Induced Emission

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер unknown

Опубликована: Фев. 13, 2024

Fluorescent polyelectrolytes have attracted tremendous attention due to their unique properties and wide applications. However, current research objects of fluorescent mainly focus on side-chain charged polyelectrolytes, the applications in plant cytomembrane imaging with long time high specificity still remain challenging. Herein, long-time targeted fluorescence cytomembranes was achieved for first using main-chain (MCCPs) aggregation-induced emission (AIE). A series MCCPs were designed synthesized, among which red-emissive AIE-active MCCP a triphenylamine linker cyano group around cationic ring-fused heterocyclic core showed best performance cells. Unlike other its neutral form polymer, this cyano-substituted conjugated polyelectrolyte can specifically target cells within short staining many advantages, including wash-free staining, photostability integrity, excellent durability (at least 12 h), low biotoxicity. In addition onion epidermal cells, AIE probe also shows good capabilities kinds such as Glycine max Vigna radiata. Such an MCCP-based system provides effective design strategy develop probes long-term ability toward plasma membranes.

Язык: Английский

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Cobalt-Catalyzed Cascade C–H Activation/Annulation Polymerizations toward Diversified and Multifunctional Sulfur-Containing Fused Heterocyclic Polymers

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(25), С. 17270 - 17284

Опубликована: Июнь 12, 2024

Transition-metal-catalyzed C–H activation has greatly benefited the synthesis and development of functional polymer materials, construction multifunctional fused (hetero)cyclic polymers via novel activation-based polyannulations emerged as a charming but challenging area in recent years. Herein, we report first cobalt(III)-catalyzed cascade activation/annulation polymerization (CAAP) approach that can efficiently transform readily available aryl thioamides internal diynes into sulfur-containing heterocyclic (SFH) polymers. Within merely 3 h, series SFH bearing complex multisubstituted S,N-doped polycyclic units are facilely produced with high molecular weights (absolute Mn up to 220400) excellent yields (up 99%), which hard achieve by traditional methods. The intermediate-terminated be used reactive macromonomer controllably extend or modify main chains. structural diversity further enriched through facile S-oxidation N-methylation reactions Benefiting from unique structures, obtained exhibit solution processability, thermal morphological stability, efficient tunable aggregate-state fluorescence, stimuli-responsive properties, UV-modulatable refractive indices 1.8464 at 632.8 nm. These properties allow potentially applied diverse fields, including metal ion detection, photodynamic killing cancer cells, fluorescent photopatterning, gradient-index optical materials.

Язык: Английский

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Synthesis of fluorescent multisubstituted polyquinolines by cascade C-H activation-based polyannulations of isonicotinamides and diynes

Science China Chemistry, Год журнала: 2023, Номер 66(4), С. 1139 - 1149

Опубликована: Март 13, 2023

Язык: Английский

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mtDNA-triggered pH response signal-amplified fluorescent probe for multiple cell discrimination

Chinese Chemical Letters, Год журнала: 2023, Номер 35(3), С. 108577 - 108577

Опубликована: Май 19, 2023

Язык: Английский

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Sequence-Defined Conjugated Oligomers in Donor–Acceptor Dyads

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(43), С. 23519 - 23526

Опубликована: Окт. 20, 2023

Conjugated macromolecules have a rich history in chemistry, owing to their chemical arrangements that intertwine physical and electronic properties. The continuing study application of these systems, however, necessitates the development atomically precise models bridge gap between molecules, polymers, and/or blends. One class conjugated polymers facilitated advancement structure-property relationships is discrete, precision oligomers remained an outstanding synthetic challenge with only handful reported examples. Here we show first synthesis molecular dyads featuring sequence-defined oligothiophene donors covalently linked small-molecule acceptor. These serve as platform for probing complex photophysical interactions involving oligomers. This assessment through unprecedented control length- sequence-dependent arrangement relative acceptor unit, made possible by incorporation hydroxyl-containing side chains at positions along backbone oligomerizations. We both sequence length play complementary roles determining transfer efficiency photoexcited states. Overall, work highlights importance spatial donor-acceptor systems are commonly studied range uses, including light harvesting photocatalysis.

Язык: Английский

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Transition-Metal-Catalyzed C–H Activation Reactions for the Creation and Modification of Organic Fluorophores

Synthesis, Год журнала: 2024, Номер 56(10), С. 1505 - 1540

Опубликована: Фев. 6, 2024

Abstract Organic fluorophores have consistently garnered significant interest owing to their widespread application across various multidisciplinary research fields. In the realm of biological research, these organic find extensive use in diverse applications such as molecular imaging, DNA sequencing, drug discovery, and biosensors. Remarkably, recent times, fluorescent molecules emerged pivotal elements advancement electronics. Across several reaction pathways developed for constructing modifying fluorophores, transition-metal-catalyzed C–H activation reactions come a dependable step-economical approach. this review we discuss activation-based approaches that been employed create modify which areas. 1 Introduction 2 Basic Reactions Creation Fluorophores 3 Merits Drawbacks Classical Modification 4 Activation/Functionalization 5 Activation Pathways Organic­ 5.1 Electrophilic 5.2 Heteroatom-Directed 6 Conclusion

Язык: Английский

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Recent progress on polymerization‐induced emission

Luminescence, Год журнала: 2023, Номер 39(2)

Опубликована: Ноя. 27, 2023

Abstract The aggregate luminescence behaviors of polymeric luminescent materials have been attracting great attention. However, the importance polymerization process on luminescence, namely, polymerization‐induced emission (PIE), has rarely overviewed. In this review, recent advances in with PIE effects are summarized, including aromatic rings based one‐/two‐/multi‐component polymerizations, and without according to disparate mechanisms polymerizations. Typical examples selected elaborate basic design principles, as well properties potential applications luminous polymers. Moreover, challenges perspectives area also discussed.

Язык: Английский

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Acidic pH‐Triggered Live‐Cell Lysosome Specific Tracking, Ratiometric pH Sensing, and Multicolor Imaging by Visible to NIR Switchable Cy‐7 Dyes

ChemBioChem, Год журнала: 2022, Номер 24(3)

Опубликована: Дек. 2, 2022

We have demonstrated an efficient synthetic route with crystal structures for the construction of acidic pH-triggered visible-to-NIR interchangeable ratiometric fluorescent pH sensors. This bioresponsive probe exhibits pH-sensitive reversible absorption/emission features, low cytotoxicity, a huge 322 nm bathochromic spectral shift augmented quantum yield from neutral to pH, high sensitivity and selective targeting ability live-cell lysosomes ideal pKa , off-to-on narrow NIR absorption/fluorescence signals molar absorption coefficient at lysosomal lumen, in-situ pH-activated imaging pH. Selective staining in human carcinoma were monitored by dual-channel confocal laser scanning microscope using pH-activatable organic dye comprising morpholine moiety lysosome openable oxazolidine ring. Moreover, real-time tracking lysosomes, 3D, multicolor been achieved synthesized probe.

Язык: Английский

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Dual colorimetric detection and chemical reduction toward silver ions of imidazole-conjugated poly(diacetylenes)

Dyes and Pigments, Год журнала: 2023, Номер 221, С. 111800 - 111800

Опубликована: Ноя. 3, 2023

Язык: Английский

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Simultaneous Imaging of pH and Peroxynitrite in the Endoplasmic Reticulum and Mitochondria: Revealing Organelle Interactions in Alzheimer’s Disease Pathogenesis

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 26, 2024

pH and peroxynitrite (ONOO–) are two critical biomarkers to unveil the corresponding status of endoplasmic reticulum (ER) stress mitochondrial dysfunction, which closely related Alzheimer's disease (AD). Simultaneously monitoring ONOO– fluctuations in ER mitochondria during AD progression is pivotal for clarifying interplay between disorders organelles revealing pathogenesis. Herein, we designed synthesized a dual-channel fluorescent probe (DCFP) visualize mitochondria. DCFP possessed excellent sensitivity selectivity without spectral crosstalk was utilized analytes within model cells larval zebrafish. Importantly, could preferentially target normal be enriched after depolarization. With aid DCFP, slower acidification rate than that induced by Aβ oligomers (AβOs) first identified, ascribed relief AβOs-triggered through Ca2+ migration from Moreover, continuous exposure AβOs led overload, accelerating overproduction As result, intracellular oxidative levels were elevated, further exacerbating aggravating turn. The advanced understanding potential this work may offer new insights methodologies studying developed also employed study other diseases dysfunction.

Язык: Английский

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