Abstract
Over
the
last
three
decades,
significant
advancements
have
been
made
in
development
of
biosensors
and
bioassays
that
use
RNA‐cleaving
DNAzymes
(RCDs)
as
molecular
recognition
elements.
While
early
examples
RCDs
were
primarily
responsive
to
metal
ions,
past
decade
has
seen
numerous
reported
for
more
clinically
relevant
targets
such
bacteria,
cancer
cells,
small
metabolites,
protein
biomarkers.
5
years
several
RCD‐based
also
evaluated
using
either
spiked
biological
matrixes
or
patient
samples,
including
blood,
serum,
saliva,
nasal
mucus,
sputum,
urine,
faeces,
which
is
a
critical
step
toward
regulatory
approval
commercialization
sensors.
In
this
review,
an
overview
methods
used
generate
properties
key
utilized
vitro
testing
first
provided.
Examples
assays
sensors
test
samples
are
then
presented,
highlighting
assay
performance
different
matrixes.
A
summary
current
prospects
challenges
diagnostic
tests
incorporating
future
directions
field
ECS Sensors Plus,
Год журнала:
2024,
Номер
3(1), С. 011601 - 011601
Опубликована: Март 1, 2024
There
is
a
plethora
of
electrochemical
biosensors
developed
for
ultrasensitive
detection
clinically
relevant
biomarkers.
However,
many
these
systems
lose
their
performance
in
heterogeneous
clinical
samples
and
are
too
complex
to
be
operated
by
end
users
at
the
point-of-care
(POC),
prohibiting
commercial
success.
Integration
with
sample
processing
technology
addresses
both
challenges;
however,
it
adds
manufacturing
complexity
overall
cost
systems.
Herein,
we
review
different
components
biosensor
avenues
creating
fully
integrated
In
context
integration,
focus
on
discussing
trade-offs
between
sensing
performance,
cost,
scalable
guide
readers
toward
designing
new
commercialization
potential.
Abstract
Infectious
diseases
present
significant
challenges
to
global
health,
thereby
extensively
affecting
both
human
society
and
the
economy.
In
recent
years,
functional
probes
have
demonstrated
remarkable
potential
as
crucial
biomedical
media
for
research
treatment
of
infectious
diseases.
Their
applications
in
realm
include
pathogen
detection,
exploration
biological
mechanisms,
development
anti‐infective
drugs.
This
review
provides
a
concise
introduction
severity,
classification,
pathogenesis
Subsequently,
we
examined
distinctiveness
design
strategies
diagnosing
treating
diseases,
shedding
light
on
their
rationale
using
typical
examples.
We
discuss
current
status
associated
with
clinical
implementation
probes.
Furthermore,
explored
prospects
these
diagnosis
aims
offer
novel
insights
into
diagnostic
broaden
disease
treatment.
Nucleic Acids Research,
Год журнала:
2023,
Номер
51(14), С. 7666 - 7674
Опубликована: Июнь 23, 2023
Abstract
The
26-mer
DNA
aptamer
(AF26)
that
specifically
binds
aflatoxin
B1
(AFB1)
with
nM-level
high
affinity
is
rare
among
hundreds
of
aptamers
for
small
molecules.
Despite
its
predicted
stem–loop
structure,
the
molecular
basis
high-affinity
recognition
AFB1
remains
unknown.
Here,
we
present
first
high-resolution
nuclear
magnetic
resonance
structure
AFB1–AF26
complex
in
solution.
to
16-residue
loop
region
aptamer,
inducing
it
fold
into
a
compact
through
assembly
two
bulges
and
one
hairpin
structure.
tightly
enclosed
within
cavity
formed
by
hairpin,
held
place
between
G·C
base
pair,
G·G·C
triple
multiple
T
bases,
mainly
strong
π–π
stacking,
hydrophobic
donor
atom–π
interactions,
respectively.
We
further
revealed
mechanism
recognizing
analogue
AFG1
only
one-atom
difference
introduced
single
mutation
at
binding
site
increase
discrimination
based
on
structural
insights.
This
research
provides
an
important
understanding
engineering,
modification
applications.
Chemical Society Reviews,
Год журнала:
2024,
Номер
53(13), С. 6830 - 6859
Опубликована: Янв. 1, 2024
This
review
presents
the
recent
progress
on
aptamers
that
have
been
explored
for
SARS-CoV-2
detection
and
therapeutics,
wherein
construction
principles
characteristics
of
aptamer-based
assembly
systems
are
systematically
summarized.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(9), С. 11809 - 11820
Опубликована: Фев. 22, 2024
Building
multifunctional
platforms
for
integrating
the
detection
and
control
of
hazards
has
great
significance
in
food
safety
environment
protection.
Herein,
bimetallic
Fe–Co-based
metal–organic
frameworks
(Fe–Co-MOFs)
peroxidase
mimics
are
prepared
applied
to
develop
a
bifunctional
platform
synergetic
sensitive
controllable
degradation
aflatoxin
B1
(AFB1).
On
one
hand,
Fe–Co-MOFs
with
excellent
peroxidase-like
activity
combined
target-induced
catalyzed
hairpin
assembly
(CHA)
construct
colorimetric
aptasensor
AFB1.
Specifically,
binding
aptamer
AFB1
releases
prelocked
Trigger
initiate
CHA
cycle
between
H2-modified
H1-tethered
magnetic
nanoparticles
form
complexes.
After
separation,
signal
supernatant
presence
TMB
H2O2
is
inversely
proportional
target
contents.
Under
optimal
conditions,
this
biosensor
enables
analysis
limit
6.44
pg/mL,
high
selectivity
satisfactory
recovery
real
samples
obtained.
other
remarkable
Fenton-like
catalytic
performance
organic
contaminants
further
used
detoxification
after
detection.
The
almost
completely
removed
within
120
min.
Overall,
introduction
improves
sensing
sensitivity;
efficient
postcolorimetric-detection
reduces
secondary
contamination
risk
experimental
operators.
This
strategy
expected
provide
ideas
designing
integrate
various
hazards.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(20)
Опубликована: Март 9, 2024
Abstract
High‐precision
viral
detection
at
point
of
need
with
clinical
samples
plays
a
pivotal
role
in
the
diagnosis
infectious
diseases
and
control
global
pandemic.
However,
complexity
that
often
contain
very
low
concentrations
makes
it
huge
challenge
to
develop
simple
diagnostic
devices
do
not
require
any
sample
processing
yet
are
capable
meeting
performance
metrics
such
as
high
sensitivity
specificity.
Herein
we
describe
new
single‐pot
single‐step
electrochemical
method
uses
real‐time
kinetic
profiling
interaction
between
high‐affinity
aptamer
an
antigen
on
surface.
This
generates
many
data
points
per
sample,
which
when
combined
machine
learning,
can
deliver
highly
accurate
test
results
short
testing
time.
We
demonstrate
this
concept
using
both
SARS‐CoV‐2
Influenza
A
viruses
model
specifically
engineered
aptamers.
Utilizing
technique
diagnose
COVID‐19
37
real
human
saliva
specificity
100
%
(27
true
negatives
10
positives,
0
false
negative
positive),
showcases
superb
precision
method.
ACS Nano,
Год журнала:
2024,
Номер
18(46), С. 31713 - 31736
Опубликована: Ноя. 7, 2024
Sensitive
and
quantitative
detection
of
chemical
biological
molecules
for
screening,
diagnosis
monitoring
diseases
is
essential
to
treatment
planning
response
monitoring.
Electrochemical
biosensors
are
fast,
sensitive,
easy
miniaturize,
which
has
led
rapid
development
in
clinical
diagnosis.
Benefiting
from
their
excellent
molecular
recognition
ability
high
programmability,
DNA
nanomaterials
could
overcome
the
Debye
length
electrochemical
by
simple
design
well
suited
as
elements
biosensors.
Therefore,
enhance
sensitivity
specificity
biosensors,
significant
progress
been
made
recent
years
optimizing
design.
Here,
establishment
sensing
strategies
based
on
reviewed
detail.
First,
structural
nanomaterial
examined
improving
overcoming
length.
In
addition,
electrical
signal
transduction
amplification
reviewed,
applications
nanomaterial-based
integrated
devices
further
summarized.
Finally,
main
opportunities
challenges
detecting
disease
biomarkers
presented
an
aim
guide
with
specificity.
Chemical Engineering Journal,
Год журнала:
2024,
Номер
495, С. 153522 - 153522
Опубликована: Июнь 26, 2024
Most
electrochemical
biosensors
require
sample
processing
to
reduce
biofouling
induced
by
the
complex
composition
of
biological
samples
that
typically
causes
a
decrease
in
target-to-blank
signal.
Herein,
we
report
zwitterionic
copolymer
bearing
sulfobetaine,
carboxylic,
aldehyde,
and
thiol
groups
as
thin
(∼16
nm)
anti-fouling
coating
for
biosensing
platforms.
The
resulting
polymer-coated
electrodes
reduced
protein
adsorption
∼
67
%
compared
bare-gold
surface
when
incubated
with
radiolabeled
human
serum
albumin
(HSA)
protein-spiked
plasma,
while
cyclic
voltammetry
yielded
5
increase
anodic
current
signal
after
incubation
1
HSA
h
83
observed
bare
gold
electrodes.
electrode
facilitated
detection
redox-labeled
DNA
buffer,
well
unprocessed
undiluted
plasma
limits
23
nM
21
nM,
respectively;
104
cp
mL−1
lentivirus
pseudotyped
Omicron
spike
SARS-CoV-2
unfiltered
50
saliva
was
also
achieved
within
min
improved
ratios
reproducibility
relative
well-established
PEG-based
platform
detecting
COVID-19.
On
this
basis,
Zwitter-repel
offers
potential
sensitively
detect
other
disease
biomarkers/analytes
eliminating
or
reducing
need
pre-processing
and/or
use
additional
backfilling/blocking
agents.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(51)
Опубликована: Окт. 31, 2023
Abstract
Here
we
report
on
an
ultra‐sensitive
colorimetric
sensing
platform
that
takes
advantage
of
both
the
strong
amplification
power
rolling
circle
(RCA)
and
high
efficiency
a
simple
urease‐mediated
litmus
test.
The
presence
target
triggers
RCA
reaction,
urease‐labelled
DNA
can
hybridize
to
biotinylated
products
be
immobilized
onto
streptavidin‐coated
magnetic
beads.
urease‐laden
beads
are
then
used
hydrolyze
urea,
leading
increase
in
pH
detected
by
We
show
this
easily
integrated
with
aptamers
for
diverse
targets
via
detection
human
thrombin
platelet‐derived
growth
factor
(PDGF)
utilizing
structure‐switching
as
well
SARS‐CoV‐2
saliva
using
spike‐binding
trimeric
aptamer.
Furthermore,
demonstrate
into
paper‐based
device
applications.
