DNA Nanomaterial-Based Electrochemical Biosensors for Clinical Diagnosis

ACS Nano, Год журнала: 2024, Номер 18(46), С. 31713 - 31736

Опубликована: Ноя. 7, 2024

Sensitive and quantitative detection of chemical biological molecules for screening, diagnosis monitoring diseases is essential to treatment planning response monitoring. Electrochemical biosensors are fast, sensitive, easy miniaturize, which has led rapid development in clinical diagnosis. Benefiting from their excellent molecular recognition ability high programmability, DNA nanomaterials could overcome the Debye length electrochemical by simple design well suited as elements biosensors. Therefore, enhance sensitivity specificity biosensors, significant progress been made recent years optimizing design. Here, establishment sensing strategies based on reviewed detail. First, structural nanomaterial examined improving overcoming length. In addition, electrical signal transduction amplification reviewed, applications nanomaterial-based integrated devices further summarized. Finally, main opportunities challenges detecting disease biomarkers presented an aim guide with specificity.

Язык: Английский

---

Chemical Engineering of DNAzyme for Effective Biosensing and Gene Therapy

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

RNA-cleaving DNAzymes are in vitro selected functional nucleic acids with inherent catalytic activities. Due to their unique properties, such as high specificity, substrate cleavage capability, and programmability, have emerged powerful tools the fields of analytical chemistry, chemical biology, biomedicine. Nevertheless, biological applications still impeded by several challenges, structural instability, compromised activity environments lack spatiotemporal control designs, which may result false-positive signals, limited efficacy or non-specific activation associated side effects. To address these various strategies been explored regulate DNAzyme through modifications, enhancing stability, selectivity, functionality, thereby positioning them ideal candidates for applications. In this review, a comprehensive overview chemically modified is provided, discussing modification effects modifications on DNAzymes. Specific examples use biosensing gene therapy also presented discussed. Finally, current challenges field addressed offer perspectives potential direction

Язык: Английский

---

Elucidating the molecular docking and binding dynamics of aptamers with spike proteins across SARS-CoV-2 variants of concern

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 14, 2025

DNA aptamers with high binding affinity against SARS-CoV-2 spike proteins have been selected and analyzed. To better understand the affinities between (S-proteins) of relevant variants concerns (VOCs), in silico vitro characterization are excellent approaches to implement. Here, we identified generated aptamer sequences targeting S-protein VOCs through systematic evolution ligands by exponential enrichment (SELEX). In , prediction was conducted, followed a step-by-step workflow for secondary tertiary structures determination, modeling, molecular docking target S-protein. The strategy limited only providing predictions possible outcomes based on scores, ranking complemented analysis using direct enzyme-linked oligonucleotides assay (ELONA), which showed dissociation constants ( K d ) within 32 nM–193 nM range across three significant VOCs. These highly specific (Alpha Apt, Delta Omicron Apt) can be further studied as potential candidates both diagnostic therapeutic applications.

Язык: Английский

---

3D Flexible SERS Biosensor based on Silver Nanoparticles modified Bacterial Nanocellulose and Gold Nanostars for Quantitative Analysis of Zearalenone

Food Control, Год журнала: 2025, Номер unknown, С. 111300 - 111300

Опубликована: Март 1, 2025

Язык: Английский

---

Direct Evolution of Matrix-Resistant Circular Bivalent DNA Aptamers for Ara h1

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

Peanut allergenic protein Ara h1 is a serious food allergen due to its potentially life-threatening effects, and accurate detection holds significant importance. While several selections were previously reported isolate DNA aptamers for h1, little success was achieved binding in complex matrix. In this work, circular bivalent obtained by employing dumbell-shaped library with dual random domains selection. The highest affinity aptamer, named as CB-APT1, exhibited dissociation constant (Kd) of 36.3 nM, determined microscale thermophoresis. Notably, similar observed even matrix that contained 80% w/w total peanut proteins. Further analysis indicates each domain acts unique moiety, working together enhance the overall affinity. Subsequently, label-free fluorescent aptasensor developed which demonstrated low limit 1.3 nM performed well, samples. Our evolution-based approach developing does not rely on structural information target protein, making it applicable wide range targets. We believe strategy can be leveraged generate diverse set high-quality are both stable functional real biological samples, thus enhancing practical applications real-world applications.

Язык: Английский

---

Selective Label-Free Detection of Imidacloprid by a Graphene Quantum Dot Fluorescent Probe

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2714 - 2714

Опубликована: Март 18, 2025

We developed a fluorescence aptamer sensing method based on gold nanoparticles and graphene quantum dots for the rapid detection of imidacloprid residues in Chinese herbal medicines. In absence imidacloprid, are dispersed solution effectively quench intensity due to protective effect aptamer. Because aptamer’s specific recognition complex forms between two compounds, no longer protected by can aggregate. Consequently, remain unquenched, resulting recovery. Under optimal conditions, showed good linear relationship with concentration range 100–3 × 104 ng/mL. The correlation coefficient was 0.9914, limit 52.42 recoveries yam, matrine, aloe leaf were 92.27–101.7%, relative standard deviation 0.45–4.14%. This has potential field applications quantitative analysis residue.

Язык: Английский

---

Aptamer-engaged nanotherapeutics against SARS-CoV-2

Discover Nano, Год журнала: 2025, Номер 20(1)

Опубликована: Апрель 27, 2025

The COVID-19 pandemic, caused by the virus SARS-CoV-2 infection, has underscored critical importance of rapid and accurate therapeutics. neutralization is paramount in controlling spread impact COVID-19. In this context, integration aptamers aptamer-related nanotherapeutics presents a valuable scientifically significant approach. Despite potential, current reviews area are often not comprehensive specific enough to encapsulate full scope therapeutic principles, strategies, advancements, challenges. This review aims fill that gap providing an in-depth examination role their related molecular medicine We first introduce unique properties, selection, recognition mechanism bind with high affinity various targets. Next, we delve into potential aptamers, focusing on ability inhibit viral entry replication, as well modulate host immune response. nucleic acid nanomedicine explored. Finally, address challenges future perspectives aptamer therapeutics, including issues stability, delivery, manufacturing scalability. conclude underscoring continued research development field meet ongoing posed pandemics. Our will be resource for researchers clinicians interested latest developments at intersection biology, nanotechnology, infectious disease management.

Язык: Английский

---

Experimentally verified flexible molecular docking and dynamic simulation of aptamer with intracellular proteins based on direct DNA 3D structure prediction

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 144318 - 144318

Опубликована: Май 1, 2025

Язык: Английский

---

Integration of polyvalent aptamers and multifunctional gold-palladium nanoparticle driven rapid and ultra-sensitive triple-mode lateral flow assay detection for tetrodotoxin

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117619 - 117619

Опубликована: Май 1, 2025

Язык: Английский

---

Nanoparticles in Subunit Vaccines: Immunological Foundations, Categories, and Applications

Small, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

Abstract Subunit vaccines, significant in next‐generation vaccine development, offer precise targeting of immune responses by focusing on specific antigens. However, this precision often comes at the cost eliciting strong and durable immunity, posing a great challenge to design. To address limitation, recent advancements nanoparticles (NPs) are utilized enhance antigen delivery efficiency boost efficacy. This review examines how physicochemical properties NPs influence various stages response during analyzes different NP types contribute activation performance. It then explores unique characteristics mechanisms these NPs, along with their advancements, highlights application subunit vaccines infectious diseases cancer. Finally, it discusses challenges NP‐based development proposes future directions for innovation promising field.

Язык: Английский

---