Yeast-Inspired Orally-Administered Nanocomposite Scavenges Oxidative Stress and Restores Gut Immune Homeostasis for Inflammatory Bowel Disease Treatment

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Excessive oxidative stress, dysregulated immune homeostasis, and disruption of the intestinal epithelial barrier are crucial features inflammatory bowel disease (IBD). Traditional treatments focusing solely on inflammation resolution remain unsatisfactory. Herein, a yeast-inspired orally administered nanocomposite was developed. First, MD@MPDA core fabricated by integrating manganese dioxide (MnO2) nanozymes onto diallyl trisulfide (H2S prodrug)-loaded mesoporous polydopamine nanoparticles (MPDA). Then, yeast cell wall (YCW) chosen to encapsulate MD@MPDA, namely, YMD@MPDA. The β-glucan embedded in YCW shell not only protected from harsh gastrointestinal environment but also allowed targeting enrichment inflamed colon. Furthermore, M1 macrophages triggered intracellular GSH-responsive H2S release pathological microenvironment. effectively alleviated responses MnO2-mediated ROS-scavenging H2S-participated immunomodulation. synergistic action contributed macrophage mitochondrial function restoration M2 polarization suppressing NOX4 signaling p38 MAPK pro-inflammatory signaling. In mice model dextran sulfate sodium (DSS)-induced IBD, multipronged manner scavenging remodeling innate adaptive reshaping gut microbiota caused YMD@MPDA ameliorated restored functions. Overall, provides promising codelivery strategy antioxidative gas prodrugs for comprehensive management IBD.

Язык: Английский

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Probiotic Delivery for Editing of the Gut Microbiota to Mitigate Colitis and Maintain Hepatic Homeostasis Via Gut–Liver Axis

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Inflammatory bowel disease (IBD) compromises the intestinal barrier and disrupts gut microbiota, impacting liver function via gut-liver axis, which in turn influences microbiota through lipid metabolites exacerbating IBD. This study introduced a probiotic-based treatment using Lactobacillus acidophilus encapsulated tungsten ion-loaded mesoporous polydopamine (LA@WMPDA) to ameliorate colitis balance enterohepatic homeostasis. After oral administration, encapsulation could protect acidophilus, scavenge reactive oxygen/nitrogen species, released ions would inhibit abnormal Enterobacteriaceae growth during colitis, consequently restoring regulating microbiota. Nontargeted metabolomics transcriptomics analyses showed increased short-chain fatty acids indole derivatives, decreased hepatic metabolism. Pathways associated with immune response, cell migration death, response bacterium significant down-regulation colon transcriptome analysis. Thus, this provided pioneered paradigm for IBD highlighted regulation of liver-related metabolic functions axis.

Язык: Английский

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The Encapsulation Strategies for Targeted Delivery of Probiotics in Preventing and Treating Colorectal Cancer: A Review

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Abstract Colorectal cancer (CRC) ranks as the third most prevalent worldwide. It is associated with imbalanced gut microbiota. Probiotics can help restore this balance, potentially reducing risk of CRC. However, hostile environment and constant changes in gastrointestinal tract pose significant challenges to efficient delivery probiotics colon. Traditional methods are often insufficient due their low viability lack targeting. To address these challenges, researchers increasingly focusing on innovative encapsulation technologies. One such approach single‐cell encapsulation, which involves applying nanocoatings individual probiotic cells. This technique improve resistance harsh environment, enhance mucosal adhesion, facilitate targeted release, thereby increasing effectiveness delivery. article reviews latest developments for CRC treatment, emphasizing potential benefits emerging techniques. also analyzes compares advantages disadvantages current Furthermore, it elucidates underlying mechanisms through prevent treat CRC, evaluates efficacy safety treatment adjuvant therapy, discusses future directions prevention.

Язык: Английский

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Antioxidant and immunomodulator nanoengineered probiotics for synergistic therapy of inflammatory bowel disease

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162678 - 162678

Опубликована: Апрель 1, 2025

Язык: Английский

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DNA Nanopatch-Specific Modification of Probiotics for Ultrasound-Triggered Inflammatory Bowel Disease Therapy

Journal of the American Chemical Society, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 7, 2024

Probiotics offer promising results for treating inflammatory bowel disease, yet precision therapy remains challenging, particularly in manipulating probiotics spatially and temporally shielding them from oxidative stress. To address these limitations, herein we synthesized bacteria-specific DNA nanopatches to modify ultrasound-triggered engineered

Язык: Английский

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Cyanobacteria–probiotics symbionts for modulation of intestinal inflammation and microbiome dysregulation in colitis

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(52)

Опубликована: Дек. 16, 2024

Inflammatory bowel disease (IBD) is often associated with excessive inflammatory response and highly dysregulated gut microbiota. Traditional treatments utilize drugs to manage inflammation, potentially probiotic therapy as an adjuvant. However, current standard practices suffer from detrimental side effects, low bioavailability, unsatisfactory therapeutic outcomes. Microbial complexes characterized by mutually beneficial symbiosis hold great promise for IBD therapy. Here, we aggregated Synechocystis sp. PCC6803 (Sp) Bacillus subtilis (BS) biomimetic mineralization form cyanobacteria–probiotics symbionts (ASp@BS), which reshaped a healthy immune system microbiota in murine model of acute colitis. The exhibited excellent tolerance the harsh environment gastrointestinal tract. Importantly, probiotics within created local anaerobic activate [NiFe]-hydrogenase enzyme cyanobacteria, facilitating production hydrogen gas (H 2 ) persistently scavenge elevated reactive oxygen species alleviate factors. resulting reduced inflammation improves viability efficiently regulate reshape intestinal barrier functions. Our research elucidates that ASp@BS leverages synergistic interaction between Sp BS create platform addresses multiple aspects IBD, offering promising comprehensive solution treatment.

Язык: Английский

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Charge-Reversal Nano-Drug Delivery Systems in the Tumor Microenvironment: Mechanisms, Challenges, and Therapeutic Applications

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9779 - 9779

Опубликована: Сен. 10, 2024

The charge-reversal nano-drug delivery system (CRNDDS) is a promising for delivering chemotherapy drugs and has gained widespread application in cancer treatment. In this review, we summarize the recent advancements CRNDDSs terms of We also delve into mechanism CRNDDSs, focusing on acid-responsive, redox-responsive, enzyme-responsive mechanisms. This study elucidates how these systems undergo charge transitions response to specific microenvironmental stimuli commonly found tumor tissues. Furthermore, review explores pivotal role diagnosis treatment, their potential limitations. By leveraging unique physiological characteristics tumors, such as acidic pH, redox potential, enzyme activity, demonstrate enhanced accumulation penetration at sites, resulting improved therapeutic efficacy diagnostic accuracy. implications highlight drug novel targeted strategy therapy diagnosis.

Язык: Английский

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Nano-armor modification of bacteria through supramolecular interactions for disease treatment

Coordination Chemistry Reviews, Год журнала: 2024, Номер 520, С. 216165 - 216165

Опубликована: Авг. 22, 2024

Язык: Английский

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Oral Microto-Nano Genome-Editing System Enabling Targeted Delivery and Conditional Activation of CRISPR-Cas9 for Gene Therapy of Inflammatory Bowel Disease

ACS Nano, Год журнала: 2024, Номер unknown

Опубликована: Авг. 31, 2024

The potent CRISPR-Cas9 technology can correct genes in human mutated cells to achieve the treatment of multiple diseases, but it lacks safe and effective delivery systems. Herein, we proposed an oral microto-nano genome-editing system aiming at enteric excessive level TNF-α for specific gene therapy inflammatory bowel disease (IBD). This editing facilitated assembly Cas9/sgRNA ribonucleoprotein (RNP) into nanoclusters (NCs) through bridging disulfide bonds. RNP-NCs were subsequently encapsulated within cell-targeted lipopolysaccharide-deleted outer membrane vesicles (dOMVs) sourced from Escherichia coli Nissle 1917, which further shielded by layer calcium alginate microspheres (CAMs). By leveraging protection effect CAMs, administration withstood gastric acid degradation upon entry stomach, achieving targeted intestines with high efficiency. As pH gradually rose, microscale CAMs swelled disintegrated, releasing nanoscale dOMVs intestines. These RNP-NCs@dOMVs could traverse mucosal barrier target macrophages where conditionally activated RNPs effectively perform genomic nucleus. Such systems represent a promising translational platform IBD.

Язык: Английский

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Surface-Functionalized Bacteria: Frontier Explorations in Next-Generation Live Biotherapeutics

Biomaterials, Год журнала: 2024, Номер 317, С. 123029 - 123029

Опубликована: Дек. 15, 2024

Язык: Английский

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