Analytical Chemistry,
Год журнала:
2024,
Номер
97(1), С. 584 - 593
Опубликована: Дек. 31, 2024
Mitochondrial
DNA
(mtDNA)
damage
is
a
prevalent
phenomenon
that
has
been
proven
to
be
implicated
in
wide
spectrum
of
diseases.
However,
the
progressive
attenuation
probe
signals
response
mtDNA
within
living
cells
inherently
limits
sensitivity
and
precision
current
probes
for
detecting
damage.
Herein,
we
employ
an
innovative
organelle
signal
ratio
imaging
approach,
utilizing
mitochondria–nucleus
migration
MCQ,
achieve
unparalleled
cells.
MCQ
exhibited
initial
preferential
binding
mtDNA,
facilitated
by
its
cationic
quinolinium
moiety,
but
migrated
nucleus
upon
This
unique
behavior
not
only
enhanced
spatial
identifiability
also
amplified
detection
significantly
harnessing
intensified
against
attenuated
mitochondrial
signal.
approach
established
positive
correlation
between
damage,
enabling
even
subtle
at
early
stage
apoptosis
with
remarkable
23-fold
enhancement
following
just
5
min
H2O2
induction
cells,
whereas
conventional
methods
relying
solely
on
fading
proved
insufficient.
Furthermore,
MCQ's
ability
monitor
occurrence
achieved
intricate
differentiation
ferroptosis.
By
monitoring
drug-induced
cancer
was
further
conducted
using
evaluate
therapeutic
efficacy
four
anticancer
drugs
very
low
concentrations.
strategy
paves
way
ultrasensitive
holds
immense
promise
damage-associated
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 2, 2025
Ferroptosis,
a
recently
proposed
form
of
regulated
cell
death,
is
characterized
by
surge
in
reactive
oxygen
species
and
subsequent
depletion
glutathione.
The
mitochondria
nucleoli
play
pivotal
roles
the
process
ferroptosis.
Therefore,
monitoring
interactions
between
during
ferroptosis
crucial
for
clarifying
its
physiological
pathological
processes.
In
this
study,
we
designed
synthesized
near-infrared
fluorescence
probe
MINU,
which
exhibits
excellent
stability
against
biological
ions
pH
environments.
Due
to
cationic
structure
good
DNA
affinity,
MINU
can
target
both
nucleoli.
Cell
imaging
demonstrates
that
reversibly
migrate
response
changes
mitochondrial
membrane
potential.
By
detecting
localization
intensity
signals,
effectively
distinguish
normal
cell,
apoptotic
ferroptotic
cell.
Monitoring
allows
us
more
accurately
appreciate
processes
Journal of the American Chemical Society,
Год журнала:
2024,
Номер
146(37), С. 25764 - 25779
Опубликована: Авг. 7, 2024
Photoresponsive
ruthenium(II)
complexes
have
recently
emerged
as
a
promising
tool
for
synergistic
photodynamic
therapy
and
chemotherapy
in
oncology,
well
antimicrobial
applications.
However,
the
limited
penetration
power
of
photons
prevents
treatment
deep-seated
lesions.
In
this
study,
we
introduce
sonoresponsive
ruthenium
complex
capable
generating
superoxide
anion
(O2•–)
via
type
I
process
initiating
ligand
fracture
upon
ultrasound
triggering.
Attaching
hydroxyflavone
(HF)
an
"electron
reservoir"
to
octahedral-polypyridyl-ruthenium
resulted
decreased
highest
occupied
molecular
orbital
(HOMO)–lowest
unoccupied
(LUMO)
energy
gaps
triplet-state
metal
charge
transfer
(3MLCT)
state
(0.89
eV).
This
modification
enhanced
generation
O2•–
under
therapeutic
irradiation
at
frequency
1
MHz.
The
produced
rapidly
induced
intramolecular
cascade
reaction
HF
fracture.
As
proof-of-concept,
engineered
Ru
into
metallopolymer
platform
(PolyRuHF),
which
could
be
activated
by
low-power
(1.5
W
cm–2,
1.0
MHz,
50%
duty
cycle)
within
centimeter
range
tissue.
activation
led
release
cytotoxic
complexes.
Consequently,
PolyRuHF
cellular
apoptosis
ferroptosis
causing
mitochondrial
dysfunction
excessive
toxic
lipid
peroxidation.
Furthermore,
effectively
inhibited
subcutaneous
orthotopic
breast
tumors
prevented
lung
metastasis
downregulating
metastasis-related
proteins
mice.
study
introduces
first
sonodynamic
therapy/sonoactivated
chemotherapy,
offering
new
avenues
deep
tumor
treatment.
Cell
membrane-coated
nanoparticle-based
delivery
systems
often
struggle
with
inevitable
drug
leakage
during
the
process
and
inefficient
release
at
tumor
site,
resulting
in
unsatisfactory
antitumor
outcomes.
Here,
we
present
an
electrostatically
stabilized
light-activated
membrane
system
(Hybrid
nanoparticles,
[Hm]@NPs)
for
leak-free
delivery,
coupled
precisely
site-specific
controllable
release,
to
elevate
cancer
treatment.
[Hm]@NPs
are
constructed
by
encapsulating
aggregation-induced
emission
(AIE)
photosensitizer
(Phenalen-1-one-quinoline
malonitrile-thiophene
tribenamine,
Phe-Qui-T)
into
a
positively
charged
reactive
oxygen
species
(ROS)-responsive
polymer
(F127-TP-U11)
form
nanoparticle
then
coating
it
negatively
hybrid
containing
red
blood
cell
Panc-1
membrane.
high
stability
effectively
prevent
through
electrostatic
interaction
between
nanoparticle.
Simultaneously,
Phe-Qui-T
light-controlled
ROS
generation
efficiently
destroys
both
ROS-responsive
membrane,
ensuring
precise
sufficient
while
enabling
photodynamic
therapy
(PDT),
thereby
augmenting
efficacy.
show
impressive
inhibition
pancreatic
mouse
models,
highlighting
potential
of
this
membrane-disruption
strategy
advanced
nanodelivery
design.
Journal of the American Chemical Society,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 20, 2025
Due
to
O2
dependence,
hypoxia-induced
apoptosis
resistance,
and
immunosuppressive
microenvironment,
the
effect
of
traditional
photodynamic
therapy
toward
hypoxic
solid
tumors
is
severely
limited.
Herein,
we
report
an
O2-independent
photocatalyst
(EBSe)
for
tumor
immunotherapy
potentiation
via
synergism
near-infrared
(NIR)
light-induced
ferroptosis/pyroptosis/oncosis.
Simple
Se
ethyl
modifications
on
methylene
blue
(MB)
endow
EBSe
with
a
remarkable
phototoxicity
enhancement
(>2500
folds)
excellent
index
(PI
>
32,000)
4T1
cells
under
hypoxia.
exhibits
self-adaptive
processes
that
generate
enhanced
type
I/II
ROS
normoxia
elevate
carbon
radical
production
Interestingly,
shows
much
higher
cell
uptake
undergoes
photoinduced
lysosomal-to-nucleus
translocation,
which
activates
ferroptosis,
pyroptosis,
oncosis.
The
three
nonapoptotic
pathways
potentiates
antitumor
immune
responses
in
tumor-bearing
mice.
This
work
offers
reliable
strategy
developing
powerful
PSs
overcome
resistance
microenvironment
tumors.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 3, 2025
We
report
a
strategy
based
on
pyridyl-anchored
organic
small-molecule
fluorescent
probes
to
develop
dual-signal
sensing
platform.
The
accomplishes
an
intelligent
integration
of
fluorescence
analysis
with
photoelectrochemical
(PEC)
sensing,
thereby
enabling
rapid
and
precise
detection
hypochlorite.
In
this
work,
the
natural
dye
chromone
was
selected
as
fluorophore
for
generating
signals.
Meanwhile,
by
using
phenothiazine
(PTZ)
specific
recognition
group
pyridine
anchoring
moiety,
we
designed
synthesized
novel
probe.
obtained
probe
used
photosensitive
material
anchored
TiO2
surface
via
N
→
Ti
bonds,
form
FTO/TiO2/FPTZ-1
heterostructure-based
platform
This
has
characteristics
high
specificity,
sensitivity,
ease
preparation,
qualitative
readout
quantitative
hypochlorite,
limit
0.288
μM
1.37
nM
PEC.
Abstract
Photodynamic
therapy
(PDT)
has
emerged
as
a
promising
protocol
for
cancer
therapy.
However,
real‐time
monitoring
of
PDT
progress
and
accurate
determination
the
optimal
treatment
timing
remain
challenges.
In
this
work,
we
selected
carbon
dots
(CDs)
new
indocyanine
green
(IR820)
building
units
to
fabricate
smart
nanotheranostics
(CDs‐IR820
assembly)
with
characteristics
controlled
release
imaging
solve
time
gap
between
diagnosis
treatment.
The
fabricated
CDs‐IR820
assembly
locked
photosensitivity
CDs
could
degrade
under
750
nm
laser
irradiation
achieve
CDs,
thus
used
cell
producing
single
oxygen
white
light.
Besides,
released
migrate
from
mitochondria
nucleus
during
process,
indicating
activity,
which
facilitated
regulation
parameters
precise
cancer.
