Pathway Complexity of Kinetically Trapped Dipeptide‐Based Metastable State: Supramolecular Structural Transformation and Helicity Tuning
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 14, 2025
Abstract
Understanding
the
complexity
of
nanostructures
involved
during
supramolecular
polymerization
process
can
be
achieved
by
kinetic
control
rather
than
thermodynamic
stability.
Study
on
pathway
and
associated
will
provide
precise
over
materials'
properties.
This
work
illustrates
structural
transformation
a
naphthalimide‐(NMI)‐conjugated
dipeptide
from
monomer
to
thermodynamically
stable
aggregated
state
in
binary
mixed
solvent
system
(DMSO
water).
The
self‐assembly
propensity
modulated
changing
ratio
water,
which
offers
an
effective
approach
stability
on‐pathway
gel
before
reaching
its
crystalline
precipitate
state.
An
in‐depth
spectroscopic
microscopic
investigation
suggested
that
initiated
formation
tiny
particles,
further
nucleated
form
helical
nanofibrilar
assembly.
At
higher
water
percentages,
showed
transient
behavior
proceeded
toward
However,
at
lower
percentage,
is
kinetically
trapped
Here,
helicity
nanofibers
altering
percentage
solvent.
self‐assembled
completely
thermoreversible
retain
chiral
memory
even
after
complete
dissolution
respective
composition.
Язык: Английский
Coiled double amyloid-like fibrils allosterically catalyze hydrolysis of beta-lactam antibiotics
Опубликована: Авг. 8, 2024
The
release
of
antibiotic
compounds
into
wastewater
constitutes
a
significant
and
growing
health
environmental
hazard,
particularly
contributing
to
the
spread
resistant
bacterial
strains.
Here,
we
demonstrate
that
amyloid
fibrils,
consisting
an
alternating
lysine/phenylalanine
-sheet
forming
short
peptide,
catalyze
hydrolysis
β-lactam
antibiotics,
most
prominent
family
compounds,
which
is
further
widespread
in
wastewater.
Peptide
variant
analysis,
molecular
dynamics
(MD)
simulations,
cryogenic
electron
microscopy
(cryo-EM)
reveal
molecules
dock
onto
fibrils’
surface
via
electrostatic
interactions
with
lysine
sidechains.
Importantly,
catalytic
occurs
allosteric
mechanism
mediated
by
unique
coiled
double
fibril
structure
anchored
are
embedded
within
twisted
fiber
strands,
facilitating
nucleophilic
attacks
Utilization
lysine-displaying
fibrils
for
hydrolytic
degradation
removal
antibiotics
from
water
was
accomplished
through
display
on
silica
beads
placed
conventional
column
filtration
setup.
Amyloid
displaying
arrays
may
furnish
versatile
platform
water,
underscoring
new
avenues
addressing
considerable
threat
contamination.
Язык: Английский
Evolving Emulsion Microcompartments via Enzyme‐Mimicking Amyloid‐Mediated Interfacial Catalysis
Small,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 13, 2024
Abstract
Living
organisms
take
in
matter
and
energy
from
their
surroundings,
transforming
these
inputs
into
forms
that
cells
can
use
to
sustain
metabolism
power
various
functions.
A
significant
advancement
the
development
of
protocells
life‐like
materials
has
been
creation
cell‐like
microcompartments
capable
evolving
higher‐order
structures
characterized
by
hierarchy
complexity.
In
this
study,
a
smart
emulsion
system
is
designed
digests
chemical
substrates
generates
organic
or
inorganic
products,
driving
self‐organization
structuration
microcompartments.
Central
lipase‐derived
peptide
undergoes
amyloid
fibrillation,
exhibiting
hydrolase‐like
activity
stabilizing
Pickering
emulsions.
Through
catalytic
hydrolysis
silicatein‐inspired
mineralization,
generate
self‐organized
surfactant
layers
silica
scaffolds
at
oil–water
interface,
respectively,
helping
prevent
coalescence.
This
process
further
facilitates
structural
evolution
high‐internal
phase
gels
are
suitable
for
direct‐ink‐writing
3D
printing.
The
findings
underscore
potential
designing
self‐evolving
soft
replicate
functions
living
organisms.
Язык: Английский