International Journal of Molecular Sciences,
Год журнала:
2018,
Номер
19(6), С. 1671 - 1671
Опубликована: Июнь 5, 2018
Cancer
is
one
of
the
leading
causes
mortality
worldwide,
because
lack
accurate
diagnostic
tools
for
early
stages
cancer.
Thus,
diagnosis,
which
provides
important
information
a
timely
therapy
cancer,
great
significance
controlling
development
disease
and
proliferation
cancer
cells
improving
survival
rates
patients.
To
achieve
goals
diagnosis
DNA
nanotechnology
may
be
effective,
since
it
has
emerged
as
valid
technique
fabrication
various
nanoscale
structures
devices.
The
resultant
DNA-based
devices
show
extraordinary
performance
in
owing
to
their
predictable
secondary
structures,
small
sizes,
high
biocompatibility
programmability.
In
particular,
rapid
nanotechnologies,
such
molecular
assembly
technologies,
endows
nanomaterials
with
more
functionalization
intellectualization.
Here,
we
summarize
recent
progress
made
functional
intelligent
highlight
prospects
this
technology
therapy.
ACS Nano,
Год журнала:
2021,
Номер
15(3), С. 3631 - 3645
Опубликована: Фев. 26, 2021
DNA
nanotechnology
has
produced
a
wide
range
of
self-assembled
structures,
offering
unmatched
possibilities
in
terms
structural
design.
Because
their
programmable
assembly
and
precise
control
size,
shape,
function,
particles
can
be
used
for
numerous
biological
applications,
including
imaging,
sensing,
drug
delivery.
While
the
biocompatibility,
programmability,
ease
synthesis
nucleic
acids
have
rapidly
made
them
attractive
building
blocks,
many
challenges
remain
to
addressed
before
using
conditions.
Enzymatic
hydrolysis,
low
cellular
uptake,
immune
cell
recognition
degradation,
unclear
biodistribution
profiles
are
yet
solved.
Rigorous
methodologies
needed
study,
understand,
fate
structures
physiological
In
this
review,
we
describe
current
faced
by
field
as
well
recent
successes,
highlighting
potential
solve
biology
problems
or
develop
smart
delivery
tools.
We
then
propose
an
outlook
drive
translation
constructs
toward
preclinical
particularly
believe
that
detailed
understanding
nanostructures
within
living
organisms,
achieved
through
thorough
characterization,
is
next
required
step
reach
clinical
maturity.
ACS Nano,
Год журнала:
2022,
Номер
16(11), С. 17552 - 17571
Опубликована: Окт. 18, 2022
With
the
total
amount
of
worldwide
data
skyrocketing,
global
storage
demand
is
predicted
to
grow
1.75
×
1014
GB
by
2025.
Traditional
methods
have
difficulties
keeping
pace
given
that
current
media
a
maximum
density
103
GB/mm3.
As
such,
production
will
far
exceed
capacity
currently
available
methods.
The
costs
maintaining
and
transferring
data,
as
well
limited
lifespans
significant
losses
associated
with
technologies
also
advanced
solutions
for
information
storage.
Nature
offers
powerful
alternative
through
defines
living
organisms
in
unique
orders
four
bases
(A,
T,
C,
G)
located
molecules
called
deoxyribonucleic
acid
(DNA).
DNA
carriers
many
advantages
over
traditional
media.
Their
high
density,
potentially
low
maintenance
cost,
ease
synthesis,
chemical
modification
make
them
an
ideal
To
this
end,
rapid
progress
has
been
made
past
decade
exploiting
user-defined
materials
encode
information.
In
review,
we
discuss
most
recent
advances
DNA-based
major
focus
on
challenges
remain
promising
field,
including
intrinsic
speed
writing
reading
cost
per
byte
stored.
Alternatively,
relying
nanostructures
(as
opposed
sequence)
other
combinations
nanomaterials
biomolecules
are
proposed
technological
economic
advantages.
summarizing
underlining
remain,
provide
roadmap
ongoing
research
rapidly
growing
which
enable
development
superior
methodologies.
Advanced Materials,
Год журнала:
2022,
Номер
34(46)
Опубликована: Июль 28, 2022
MicroRNA
(miR)-based
therapy
shows
strong
potential;
however,
structural
limitations
pose
a
challenge
in
fully
exploiting
its
biomedical
functionality.
Tetrahedral
framework
DNA
(tFNA)
has
proven
to
be
an
ideal
vehicle
for
miR
therapy.
Inspired
by
the
ancient
Chinese
myth
"Sun
and
Immortal
Birds,"
novel
bioswitchable
inhibitor
delivery
system
(BiRDS)
is
designed
with
three
inhibitors
(the
immortal
birds)
nucleic
acid
core
central
sun).
The
BiRDS
fuses
within
framework,
maximizing
their
loading
capacity,
while
allowing
retain
characteristics
of
small-sized
tFNA
avoiding
uncertainty
associated
RNA
exposure
traditional
protocols.
RNase
H-responsive
sequence
at
tail
each
"immortal
bird"
enables
transform
from
3D
2D
structure
upon
entering
cells,
promoting
inhibitors.
To
confirm
application
potential,
used
deliver
miR-31
inhibitor,
antiaging
effects
on
hair
follicle
stem
into
skin
aging
model.
Superior
penetration
ability
are
observed
significant
anti-aging
effects.
These
findings
demonstrate
capability
editability
improve
stability
efficacy
miRs
future
innovations.
ACS Nano,
Год журнала:
2022,
Номер
16(7), С. 10496 - 10508
Опубликована: Июнь 17, 2022
Fabrication
of
nanoscale
DNA
devices
to
generate
3D
nano-objects
with
precise
control
shape,
size,
and
presentation
ligands
has
shown
tremendous
potential
for
therapeutic
applications.
The
interactions
between
the
cell
membrane
different
topologies
nanostructures
are
crucial
designing
efficient
tools
interfacing
biological
systems.
practical
applications
these
nanocages
still
limited
in
cellular
systems
owing
understanding
their
interaction
endocytic
pathway.
correlation
geometry
internalization
efficiency
remains
elusive.
We
investigated
influence
shape
size
on
efficiency.
found
that
one
particular
geometry,
i.e.,
tetrahedral
is
more
favored
over
other
designed
geometries
uptake
2D
models.
This
also
replicable
processes
like
invasion
assays
a
spheroid
model,
passing
epithelial
barriers
vivo
zebrafish
model
Our
work
provides
detailed
information
rational
design
nanodevices
upcoming
biomedical
Advanced Materials,
Год журнала:
2023,
Номер
35(30)
Опубликована: Фев. 5, 2023
Therapeutic
DNAzymes
have
unceasingly
intrigued
the
scientific
community
owing
to
their
prosperous
gene
regulation
capability.
The
efficacy
of
against
many
types
diseases
has
been
extensively
studied
for
over
two
decades.
However,
high
expectations
are
still
not
translated
clinic
because
low
effectiveness
in
vivo.
Over
last
five
years,
several
aspects
considered
optimize
DNAzyme-integrated
therapeutics,
including
structural
stability,
mechanism
exploration,
cell
internalization
rate,
cofactor
activation,
and
off-target
effects.
Hence,
this
review
first
discusses
early
monotherapy
design
DNAzymes.
Subsequently,
latest
modes
action
reviewed,
followed
by
an
elaboration
on
stabilization
strategies
considering
catalytic
core
substrate-binding
arms.
DNAzyme-based
synergistic
therapy
is
then
examined,
highlighting
responsive
carrier
construction,
effects,
discovered
advanced
functions,
concerns.
Beyond
this,
key
clinical
advances
elucidated
showcasing
progress.
Finally,
future
trends
development
challenges
DNAzyme-powered
therapeutics
coming
years
discussed
detail.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Фев. 23, 2023
DNA
origami
may
enable
more
versatile
gene
delivery
applications
through
its
ability
to
create
custom
nanoscale
objects
with
specific
targeting,
cell-invading,
and
intracellular
effector
functionalities.
Toward
this
goal
here
we
describe
the
expression
of
genes
folded
in
delivered
mammalian
cells.
Genes
readily
express
from
custom-sequence
single-strand
scaffolds
within
objects,
provided
that
can
denature
cell.
We
demonstrate
enhanced
efficiency
by
including
tuning
multiple
functional
sequences
structures,
virus-inspired
inverted-terminal
repeat-like
(ITR)
hairpin
motifs
upstream
or
flanking
cassette.
gene-encoding
bricks
assemble
into
multimeric
stoichiometrically
controlled
co-delivery
same
Our
work
provides
a
framework
for
exploiting
applications.
Nature Nanotechnology,
Год журнала:
2023,
Номер
18(11), С. 1319 - 1326
Опубликована: Авг. 17, 2023
Multispecific
antibodies
have
emerged
as
versatile
therapeutic
agents,
and
therefore,
approaches
to
optimize
streamline
their
design
assembly
are
needed.
Here
we
report
on
the
modular
programmable
of
IgG
antibodies,
F(ab)
scFv
fragments
DNA
origami
nanocarriers.
We
screened
105
distinct
quadruplet
antibody
variants
in
vitro
for
ability
activate
T
cells
presence
target
cells.
T-cell
engagers
were
identified,
which
showed
specific
efficient
T-cell-mediated
lysis
five
cell
lines.
used
these
lyse
tumour
vivo
a
xenograft
mouse
model.
Our
approach
enables
rapid
generation,
screening
testing
bi-
multispecific
facilitate
preclinical
pharmaceutical
development
from
discovery
proof
concept.
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(13)
Опубликована: Фев. 3, 2023
Abstract
Bispecific
chimeras
bridging
cell
membrane
proteins
with
lysosome‐trafficking
receptors
(LTRs)
provide
an
effective
therapeutic
approach
through
lysosomal
degradation
of
disease‐relevant
targets.
Here,
we
report
a
novel
dendronized
DNA
chimera
(DENTAC)
strategy
that
uses
dendritic
to
engage
surface
scavenger
(SRs)
as
LTR.
Using
bioorthogonal
strain‐promoted
alkyne‐azide
cycloaddition
conjugate
the
protein
binder,
resulting
DENTAC
is
able
traffic
target
into
lysosome
for
elimination.
We
demonstrated
utility
by
degrading
oncogenic
nucleolin
(NCL)
and
epidermal
growth
factor
receptor
(EGFR).
The
anti‐cancer
application
NCL‐targeting
was
validated
in
mouse
xenograft
model
lung
cancer.
This
work
thus
presents
new
avenue
rapid
development
potent
degraders
against
proteins,
also
broad
research
prospects.
