Addressing the in vivo delivery of nucleic-acid nanostructure therapeutics DOI

Shana Alexander,

Mohammadamir G. Moghadam,

Meghan Rothenbroker

и другие.

Advanced Drug Delivery Reviews, Год журнала: 2023, Номер 199, С. 114898 - 114898

Опубликована: Май 23, 2023

Язык: Английский

Recent Advances in DNA Origami-Engineered Nanomaterials and Applications DOI Creative Commons
Pengfei Zhan, Andreas Peil, Qiao Jiang

и другие.

Chemical Reviews, Год журнала: 2023, Номер 123(7), С. 3976 - 4050

Опубликована: Март 29, 2023

DNA nanotechnology is a unique field, where physics, chemistry, biology, mathematics, engineering, and materials science can elegantly converge. Since the original proposal of Nadrian Seeman, significant advances have been achieved in past four decades. During this glory time, origami technique developed by Paul Rothemund further pushed field forward with vigorous momentum, fostering plethora concepts, models, methodologies, applications that were not thought before. This review focuses on recent progress origami-engineered nanomaterials five years, outlining exciting achievements as well unexplored research avenues. We believe spirit assets Seeman left for scientists will continue to bring interdisciplinary innovations useful next decade.

Язык: Английский

Процитировано

159

Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds DOI Creative Commons
Eike‐Christian Wamhoff, Larance Ronsard, Jared Feldman

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Янв. 30, 2024

Protein-based virus-like particles (P-VLPs) are commonly used to spatially organize antigens and enhance humoral immunity through multivalent antigen display. However, P-VLPs thymus-dependent that themselves immunogenic can induce B cell responses may neutralize the platform. Here, we investigate thymus-independent DNA origami as an alternative material for display using receptor binding domain (RBD) of SARS-CoV-2 spike protein, primary target neutralizing antibody responses. Sequential immunization mice with DNA-based VLPs (DNA-VLPs) elicits protective antibodies in a manner depends on valency displayed T help. Importantly, immune sera do not contain boosted, class-switched against scaffold, contrast elicit strong memory both scaffold. Thus, DNA-VLPs immunogenicity without generating scaffold-directed thereby offer important particulate vaccine design.

Язык: Английский

Процитировано

43

A DNA robotic switch with regulated autonomous display of cytotoxic ligand nanopatterns DOI Creative Commons
Yang Wang, Igor Baars, Ieva Berzina

и другие.

Nature Nanotechnology, Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

The clustering of death receptors (DRs) at the membrane leads to apoptosis. With goal treating tumours, multivalent molecular tools that initiate this mechanism have been developed. However, DRs are also ubiquitously expressed in healthy tissue. Here we present a stimuli-responsive robotic switch nanodevice can autonomously and selectively turn on display cytotoxic ligand patterns tumour microenvironments. We demonstrate switchable DNA origami normally hides six ligands but displays them as hexagonal pattern 10 nm diameter once under higher acidity. This effectively cluster trigger apoptosis human breast cancer cells pH 6.5 while remaining inert 7.4. When administered mice bearing xenografts, decreased growth by up 70%. data feasibility opportunities for developing switches path targeted treatment.

Язык: Английский

Процитировано

19

Models and simulations of structural DNA nanotechnology reveal fundamental principles of self-assembly DOI Creative Commons
Alexander Cumberworth,

Aleks Reinhardt

Chemical Society Reviews, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

We explore how simulations and modelling help us to learn about the fundamental principles of DNA self-assembly; we highlight key concepts such as cooperativity nucleation show they can be harnessed improve self-assembly process.

Язык: Английский

Процитировано

4

Inspired Beyond Nature: Three Decades of Spherical Nucleic Acids and Colloidal Crystal Engineering with DNA DOI
Chad A. Mirkin, Sarah Hurst Petrosko

ACS Nano, Год журнала: 2023, Номер 17(17), С. 16291 - 16307

Опубликована: Авг. 16, 2023

The conception, synthesis, and invention of a nanostructure, now known as the spherical nucleic acid, or SNA, in 1996 marked advent new field chemistry. Over past three decades, SNA its analogous anisotropic equivalents have provided an avenue for us to think about some most fundamental concepts chemistry ways led technologies that are significantly impacting fields from medicine materials science. A prime example is colloidal crystal engineering with DNA, framework using SNAs related structures synthesize programmable matter. Herein, we document evolution this framework, which was initially inspired by nature, describe how it allows researchers chart paths move beyond it, matter real-world significance envisioned created.

Язык: Английский

Процитировано

29

Evaluation of Nonmodified Wireframe DNA Origami for Acute Toxicity and Biodistribution in Mice DOI Creative Commons
Eike‐Christian Wamhoff, Grant A. Knappe,

Aurora A. Burds

и другие.

ACS Applied Bio Materials, Год журнала: 2023, Номер 6(5), С. 1960 - 1969

Опубликована: Апрель 11, 2023

Wireframe DNA origami can be used to fabricate virus-like particles for a range of biomedical applications, including the delivery nucleic acid therapeutics. However, acute toxicity and biodistribution these wireframe nanoparticles (NANPs) have not been previously characterized in animal models. In present study, we observed no indications BALB/c mice following therapeutically relevant dosage nonmodified DNA-based NANPs via intravenous administration, based on liver kidney histology, biochemistry, body weight. Further, immunotoxicity was minimal, as indicated by blood cell counts type-I interferon pro-inflammatory cytokines. an SJL/J model autoimmunity, NANP-mediated DNA-specific antibody response or immune-mediated pathology intraperitoneal administration NANPs. Finally, studies revealed that accumulate within one hour, concomitant with substantial renal clearance. Our observations support continued development next-generation therapeutic platforms.

Язык: Английский

Процитировано

27

Nucleic Acid‐based Enzyme Cascades—Current Trends and Future Perspectives DOI Creative Commons

Sandra Kröll,

Christof M. Niemeyer

Angewandte Chemie International Edition, Год журнала: 2023, Номер 63(5)

Опубликована: Окт. 23, 2023

Abstract The natural micro‐ and nanoscale organization of biomacromolecules is a remarkable principle within living cells, allowing for the control cellular functions by compartmentalization, dimensional diffusion substrate channeling. In order to explore these biological mechanisms harness their potential applications such as sensing catalysis, molecular scaffolding has emerged promising approach. case synthetic enzyme cascades, developments in DNA nanotechnology have produced particularly powerful scaffolds whose addressability can be programmed with nanometer precision. this minireview, we summarize recent field biomimetic multicatalytic cascade reactions organized on nanostructures. We emphasize impact underlying design principles like origami, efficient strategies immobilization, well importance experimental parameters theoretical modeling. show how nanostructures enabled better understanding compartmentalization effects at length scale, discuss challenges future commercial applications.

Язык: Английский

Процитировано

24

A physics-informed neural network for Kresling origami structures DOI
Chen‐Xu Liu, Xinghao Wang, Weiming Liu

и другие.

International Journal of Mechanical Sciences, Год журнала: 2024, Номер 269, С. 109080 - 109080

Опубликована: Фев. 4, 2024

Язык: Английский

Процитировано

17

Self‐Assembled DNA Nanospheres Driven by Carbon Dots for MicroRNAs Imaging in Tumor via Logic Circuit DOI

Qiutong Chen,

Xinyi Xia,

Zhigang Liang

и другие.

Small, Год журнала: 2024, Номер 20(25)

Опубликована: Янв. 17, 2024

Abstract DNA nanostructures with diverse biological functions have made significant advancements in biomedical applications. However, a universal strategy for the efficient production of is still lacking. In this work, facile and mild method presented self‐assembling polyethylenimine‐modified carbon dots (PEI‐CDs) into nanospheres called CANs at room temperature. This makes universally applicable to multiple applications involving various types DNA. Due ultra‐small size strong cationic charge PEI‐CDs, exhibit dense structure high loading capacity encapsulated while providing excellent stability by protecting from enzymatic hydrolysis. Additionally, Mg 2+ incorporated form Mg@CANs which enriches performance enables subsequent imaging exogenous . Especially, DNAzyme logic gate system that contains AND OR constructed successfully delivered tumor cells vitro vivo. They can be specifically activated endogenic human apurinic/apyrimidinic endonuclease 1 recognize expression levels miRNA‐21 miRNA‐155 sites biocomputing. A versatile pattern delivery flexible circuits miRNAs are developed.

Язык: Английский

Процитировано

15

Dominant Analytical Techniques in DNA Nanotechnology for Various Applications DOI
Kayla Neyra, Heather R. Everson, Divita Mathur

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(9), С. 3687 - 3697

Опубликована: Фев. 14, 2024

DNA nanotechnology is rapidly gaining traction in numerous applications, each bearing varying degrees of tolerance to the quality and quantity necessary for viable nanostructure function. Despite distinct objectives application, they are united their reliance on essential analytical techniques, such as purification characterization. This tutorial aims guide reader through current state chemistry, outlining important factors consider when designing, assembling, purifying, characterizing a downstream applications.

Язык: Английский

Процитировано

14